Identification of nematodes of the genus Teladorsagia parasites of ruminants with the help of species-specific markers based on ITS2 rDNA

Abstract

The present study delves into a methodological framework aimed at establishing species-specific markers via the utilization of sequences derived from the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA. This method, in conjunction with polymerase chain reaction (PCR) testing, serves as a diagnostic tool for discerning species belonging to the genus Teladorsagia Andreeva et Satubaldin, 1954. These species, constituents of the subfamily Ostertagiinae (Nematoda: Trichostrongylidae), exhibit wide distribution within the gastrointestinal tracts of ruminants across the geographic expanse of Uzbekistan. The heart of this endeavor is the development of species-specific primers, a pioneering creation in its own right. These primers are crafted using sequences emanating from the ITS2 region of the ribosomal DNA, an innovative approach that facilitates the precise identification of morphospecies within the Teladorsagia genus. Notably, the primers exhibit a nucleotide length of 153 base pairs, an attribute instrumental in their capacity to accurately distinguish and diagnose eggs and larvae of three distinct morphspecies: T. circumcincta, T. trifurcata, and T. davtiani. The potential implications of this method are significant, with ramifications reverberating across the field of veterinary diagnostics. Through the application of these primers, practitioners and researchers alike can effectively ascertain the presence of specific Teladorsagia morphospecies in ruminant animals. This holds the promise of not only enhancing diagnostic precision but also contributing to the broader comprehension of the prevalence and distribution of these nematode species within the local ruminant populations.

Morphological and molecular characterization of root-knot nematodes from Uzbekistan

“…It is important to choose the correct and accurate method to identify different species of nematodes, including RKN (Den Nijs & Van den Berg, 2012). RKN and other nematodes have been identified based on morphological characters, host-plant response, isozyme analyses and molecular techniques (Kiewnick et al, 2014;Álvarez-Ortega et al, 2019;Sato et al, 2019;Kuchboev et al, 2020;Ibrokhimov et al, 2023). Currently, a number of molecular methods have been developed to identify species of RKN.…”