Identification of neuropeptide Y in superior cervical ganglion neurons that project to the oesophagus – A combined immunohistochemical labelling and retrograde tracing study in pigs

Rytel, Liliana; Snarska, Anna; Gonkowski, Sławomir; Wojtkiewicz, Joanna; Szenci, O.; Sobiech, Przemysław

doi:10.1556/004.2019.011

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…Although no longer widely used for tracing projections in the central nervous system, they (along with organic tracers such as CTb, WGA, etc.) remain popular choices for the identification of autonomic, motor or sensory innervation of peripheral targets, into which they can be injected in relatively large volumes (Furukawa et al, 2008; Zele et al, 2010; Yu et al, 2015; Pidsudko et al, 2019; Rytel et al, 2019). Interestingly, because they are not dependent on active transport, inorganic dyes may also be used for identification of projections in fixed tissue post mortem .…”

Section: Anterograde and Retrograde Tracersmentioning

confidence: 99%

A Student’s Guide to Neural Circuit Tracing

et al. 2019

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The mammalian nervous system is comprised of a seemingly infinitely complex network of specialized synaptic connections that coordinate the flow of information through it. The field of connectomics seeks to map the structure that underlies brain function at resolutions that range from the ultrastructural, which examines the organization of individual synapses that impinge upon a neuron, to the macroscopic, which examines gross connectivity between large brain regions. At the mesoscopic level, distant and local connections between neuronal populations are identified, providing insights into circuit-level architecture. Although neural tract tracing techniques have been available to experimental neuroscientists for many decades, considerable methodological advances have been made in the last 20 years due to synergies between the fields of molecular biology, virology, microscopy, computer science and genetics. As a consequence, investigators now enjoy an unprecedented toolbox of reagents that can be directed against selected subpopulations of neurons to identify their efferent and afferent connectomes. Unfortunately, the intersectional nature of this progress presents newcomers to the field with a daunting array of technologies that have emerged from disciplines they may not be familiar with. This review outlines the current state of mesoscale connectomic approaches, from data collection to analysis, written for the novice to this field. A brief history of neuroanatomy is followed by an assessment of the techniques used by contemporary neuroscientists to resolve mesoscale organization, such as conventional and viral tracers, and methods of selecting for sub-populations of neurons. We consider some weaknesses and bottlenecks of the most widely used approaches for the analysis and dissemination of tracing data and explore the trajectories that rapidly developing neuroanatomy technologies are likely to take.

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