Identification of neutralizing antigenic sites on VP1 and VP2 of type A5 foot-and-mouth disease virus, defined by neutralization-resistant variants

Saiz, Juan-Carlos; González, Miguel; Borca, Manuel V.; Sánchez‐Madrid, Francisco; Moore, Douglas M.

doi:10.1128/jvi.65.5.2518-2524.1991

Cited by 62 publications

(31 citation statements)

References 43 publications

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“…This has allowed the definition of multiple antigenic sites on FMDV, depending on the virus serotype analyzed. Most of these antigenic sites have been located on VP1, but recently neutralizing epitopes located outside (or at least partially outside) VP1 have been described for serotypes 0 (3, 26) and A (7,8,31,38).…”

mentioning

confidence: 99%

“…Production and characterization of the MAbs used have been described elsewhere (32). MAb-resistant variants 1-HA6 and 1-OG2 were obtained from the parental virus population (A5 Sp-86) as previously described (31). Serial passages of the variant strains in the absence of neutralizing MAbs were made on monolayers of a continuous bovine kidney cell line (LFBK; 37) in 25-cm2 tissue culture flasks, using a multiplicity of infection of 1 to 3 PFU per cell.…”

mentioning

confidence: 99%

“…Viruses from different passages or coinfections were amplified in monolayers of BHK-21 cells and purified as previously described (5,40). FMDV RNA was prepared from purified virus as described by Grubman and Baxt (25), and sequencing reactions were performed by the primer extension-dideoxynucleotide chain termination method, using oligonucleotide primers sited in the P1 region of the genome as previously described (7,31). Sequence information was stored and analyzed by using software provided under license from the Genetics Computer Group, Madison, Wis. (14).…”

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confidence: 99%

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Antigenic stability of foot-and-mouth disease virus variants on serial passage in cell culture

González

Saiz

Laor

et al. 1991

J Virol

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Two neutralizing monoclonal antibody (MAb)-resistant variants selected from an isolate of foot-and-mouth disease virus (FMDV) type A5 were repeatedly passaged in cell culture and monitored for susceptibility to neutralization by the selecting MAb. A variant isolated with a MAb to a conformational epitope (1-OG2) lost resistance in 20 passages, while a variant isolated with a MAb to a linear epitope (1-HA6) persisted for 30 passages. In both cases, the virus population emerging after passage was antigenicaily and genetically indistinguishable from the original wild-type parental virus (FMDV A5 Spain-86). Coinfection assays with the wild type and each variant, and between the variants, showed rapid conversion to a homogeneous population. Wild-type virus prevailed over the variants and for coinfection between the variants, the linear epitope variant 1-HA6. While both variants arose from a single nucleotide substitution and reversion to wild type occurred for each, it appears that the variant based on the continuous epitope (1-HA6) was more stable. We discuss the implications of these results for the antigenic diversity of FMDV and its relationship to virus evolution.

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Antigenic stability of foot-and-mouth disease virus variants on serial passage in cell culture

González

Saiz

Laor

et al. 1991

J Virol

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“…Again in 1986, a single outbreak of FMDV A occurred in Toledo, Spain. Analysis of the complete VP1 sequence showed a close relationship to A 5 /Allier/FRA/60, a vaccine strain in use in Spain at the time (Saiz et al, 1991; Fig. 1).…”

Section: Fmd Outbreaks In Italy and Spainmentioning

confidence: 81%

Incursions of Foot-and-Mouth Disease Virus into Europe between 1985 and 2006

Valarcher¹,

Leforban

Rweyemamu

et al. 2008

Transboundary and Emerging Diseases

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Foot-and-mouth disease (FMD) is one of the biggest threats to animal health in European countries. In the last 22 years (1985-2006), FMD has occurred 37 times in 14 European countries. Serotype O was most frequently involved in these outbreaks followed by A, C and Asia 1. Sometimes, epidemics were very limited and at other times, they were the cause of devastating economic losses. In most cases (22/37), the origin of the outbreaks could not be determined. For some of these outbreaks, however, routes of introduction and spread were identified through epidemiological inquiries. Moreover, in some cases, the origin of the virus was also traced by phylogenetic analysis of the partial or complete sequences of VP1 genes. Lessons learned from the outbreaks are still useful as most of the same risk factors persist. However, efforts made by FMD-free countries to help those where the disease is endemic are a valuable strategy for the reduction of the global risk. The present and the future potential sources of FMD infection need to be identified to best focus European efforts.

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“…The epitopes on VP1, VP2 and VP3 are recognized by neutralizing antibodies. [11][12][13] In particular, VP1 bears the greatest number of neutralizing epitopes. [14][15][16] Serologically, EV is categorized into 66 or more serotypes on the basis of neutralization tests using prototype viruses.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a panel of in‐house polyclonal antibodies for the diagnosis of enterovirus infections

et al. 2014

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The aim of this study was to establish a reliable method of virus detection for the diagnosis of critical enterovirus infections such as acute infective encephalitis, encephalomyelitis and myocarditis. Because histopathological and immunohistochemical analyses of paraffin-embedded tissues play an important role in recognizing infectious agents in tissue samples, six in-house polyclonal antibodies raised against three representative enteroviruses using an indirect immunofluorescence assay and immunohistochemistry were examined. This panel of polyclonal antibodies recognized three serotypes of enterovirus. Two of the polyclonal antibodies were raised against denatured virus particles from enterovirus A71, one was raised against the recombinant VP1 protein of coxsackievirus B3, and the other for poliovirus type 1 were raised against denatured virus particles, the recombinant VP1 protein and peptide 2C. Western blot analysis revealed that each of these antibodies recognized the corresponding viral antigen and none cross-reacted with non-enteroviruses within the family Picornaviridae. However, all cross-reacted to some extent with the antigens derived from other serotypes of enterovirus. Indirect immunofluorescence assay and immunohistochemistry revealed that the virus capsid and non-structural proteins were localized in the cytoplasm of affected culture cells, and skeletal muscles and neurons in neonatal mice experimentally-infected with human enterovirus. The antibodies also recognized antigens derived from recent clinical isolates of enterovirus A71, coxsackievirus B3 and poliovirus. In addition, immunohistochemistry revealed that representative antibodies tested showed the same recognition pattern according to each serotype. Thus, the panel of in-house anti-enterovirus polyclonal antibodies described herein will be an important tool for the screening and pathological diagnosis for enterovirus infections, and may be useful for the classification of different enterovirus serotypes, including coxsackieviruses A and B, echoviruses, enterovirus A71 and poliovirus.

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Identification of neutralizing antigenic sites on VP1 and VP2 of type A5 foot-and-mouth disease virus, defined by neutralization-resistant variants

Cited by 62 publications

References 43 publications

Antigenic stability of foot-and-mouth disease virus variants on serial passage in cell culture

Antigenic stability of foot-and-mouth disease virus variants on serial passage in cell culture

Incursions of Foot-and-Mouth Disease Virus into Europe between 1985 and 2006

Establishment of a panel of in‐house polyclonal antibodies for the diagnosis of enterovirus infections

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