Identification of new astroviruses in synanthropic squamates

Diakoudi, Georgia; Buonavoglia, Alessio; Pellegrini, Francesco; Capozza, Paolo; Vasinioti, Violetta Iris; Cardone, Roberta; Catella, Cristiana; Camero, Michele; Parisi, Antonio; Capozzi, Loredana; Mendoza‐Roldan, Jairo Alfonso; Otranto, Domenico; Bányai, Krisztiàn; Martella, Vito; Lanave, Gianvito

doi:10.1016/j.rvsc.2023.06.009

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Astroviruses (belonging to the Astroviridae family, genus Avastrovirus) are small, single-stranded, positive-sense, nonenveloped RNA viruses with a nonsegmented RNA genome approximately 6.0-7.7 kb in length; infection often leads to coinfections with many other enteric viruses, for instance, adenoviruses and noroviruses [1,2]. The astrovirus genome contains a 5 ′ -untranslated region (UTR), a 3 ′ -UTR, 3 ′ open reading frames (ORFs), and a poly(A) tail [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

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The Development of a Sensitive Droplet Digital Polymerase Chain Reaction Test for Quantitative Detection of Goose Astrovirus

Shi,

Jin,

Zhang

et al. 2024

Viruses

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(1) Goose astrovirus (GAstV) is a novel emerging pathogen that causes significant economic losses in waterfowl farming. A convenient, sensitive, and specific detection method for GAstV in field samples is important in order to effectively control GAstV. Droplet digital polymerase chain reaction (ddPCR) is a novel, sensitive, good-precision, and absolute quantitation PCR technology which does not require calibration curves. (2) In this study, we developed a ddPCR system for the sensitive and accurate quantification of GAstV using the conserved region of the ORF2 gene. (3) The detection limit of ddPCR was 10 copies/µL, ~28 times greater sensitivity than quantitative real-time PCR (qPCR). The specificity of the test was determined by the failure of amplification of other avian viruses. Both ddPCR and qPCR tests showed good repeatability and linearity, and the established ddPCR method had high sensitivity and good specificity to GAstV. Clinical sample test results showed that the positive rate of ddPCR (88.89%) was higher than that of qPCR (58.33%). (4) As a result, our results suggest that the newly developed ddPCR method might offer improved analytical sensitivity and specificity in its GAstV measurements. The ddPCR could be widely applied in clinical tests for GAstV infections.

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Section: Introductionmentioning

confidence: 99%

“…The genome is organized into 3 ORFs (ORF1a, ORF1b, and ORF2). Among them, ORF1a and ORF1b encode viral nonstructural proteins, and ORF2 encodes a viral structural protein (capsid) [1]. The host range of astroviruses is wide, and the main symptoms are diarrhea and intestinal disease [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

The Development of a Sensitive Droplet Digital Polymerase Chain Reaction Test for Quantitative Detection of Goose Astrovirus

Shi,

Jin,

Zhang

et al. 2024

Viruses

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“…The host range of astroviruses is comprised of many mammalian species, such as cows ( 8 ), buffalo calves ( 9 , 10 ), sheep ( 11 ), pigs ( 12 ), cats ( 13 ), mink ( 14 ), bats ( 15 ), red deer ( 16 ), urban brown rats ( 17 ), synanthropic squamates ( 18 ), California sea lions, Steller’s sea lions, and bottlenose dolphins ( 19 ), as well as certain avian species (chicken, turkey, duck) ( 20 , 21 ). Infected hosts present the main clinical features of gastrointestinal diseases (GIDs).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of canine astrovirus in non-diarrhea dogs and diarrhea dogs in Vietnam and Thailand reveals the presence of a unique lineage

Nguyen,

Piewbang,

Techangamsuwan

2023

Front. Vet. Sci.

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The role of canine astrovirus (CaAstV) in canine gastrointestinal disease (GID) is unknown. In this study, a total of 327 fecal swab (FS) samples were collected, including 113 FSs in Vietnam (46 samples from healthy dogs and 67 samples from GID dogs) and 214 FSs in Thailand (107 samples from healthy dogs and 107 samples from GID dogs). Overall, the prevalence of CaAstV in Vietnam and Thailand was 25.7% (29/113) and 8.9% (19/214), respectively. CaAstV was detected in both non-diarrhea dogs (21.7 and 7.5%) and diarrhea dogs (28.4% and 10.3%), respectively, in Vietnam and Thailand. In both countries, CaAstV was frequently detected in puppies under 6 months of age (23.3%) (p = 0.02). CaAstV-positive samples in Vietnam and Thailand were identified as co-infected with canine parvovirus, canine enteric coronavirus, canine distemper virus, and canine kobuvirus. The complete coding sequence of seven Vietnamese CaAstV and two Thai CaAstV strains were successfully characterized. Phylogenetic analyses showed that Vietnamese and Thai CaAstV strains were genetically close to each other and related to the Chinese strains. Furthermore, analysis of complete coding sequences indicated that the OR220030_G21/Thailand/2021 strain formed a unique lineage, whereas no recombination event was found in this study, suggesting that this strain might be an original lineage. In summary, this is the first study to report the presence of CaAstV in dogs with and without diarrhea in Vietnam and Thailand, and it was most often found in puppies with diarrhea. Our results highlight the importance of the CaAstV in dog populations and the need for continued surveillance of these emerging pathogens.

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Integrative transcriptomics and proteomics analysis provide a deep insight into goose astrovirus-host interactions during GAstV infection

Shi,

Jin,

Zhao

et al. 2024

Poultry Science

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Identification of new astroviruses in synanthropic squamates

Cited by 4 publications

References 34 publications

The Development of a Sensitive Droplet Digital Polymerase Chain Reaction Test for Quantitative Detection of Goose Astrovirus

The Development of a Sensitive Droplet Digital Polymerase Chain Reaction Test for Quantitative Detection of Goose Astrovirus

Genetic characterization of canine astrovirus in non-diarrhea dogs and diarrhea dogs in Vietnam and Thailand reveals the presence of a unique lineage

Integrative transcriptomics and proteomics analysis provide a deep insight into goose astrovirus-host interactions during GAstV infection

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