Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell division gene ftsQ

Begg, K J; Hatfull, Graham F.; Donachie, W.

doi:10.1128/jb.144.1.435-437.1980

Cited by 93 publications

(21 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To avoid potential problems caused by the wide differences in the copy number of septal proteins, all antibodies have been checked after purification to adapt their working dilution to a level adequate for the detection of rings in unperturbed cells of the wild type strains. The antibody specificity was also confirmed by immunolocalization of each protein in its respective conditional mutant strain under permissive or restrictive conditions: VIP205 (Garrido et al, 1993) for FtsZ, OV16 (Donachie et al, 1979) for FtsA, CH5/pCH32 (Hale and de Boer, 1997) for ZipA, TOE44 (Begg et al, 1980) for FtsK, TOE1 (Begg et al, 1980) for FtsQ and JOE565 (Chen and Beckwith, 2001) for FtsN.…”

Section: Polyclonal Antiseramentioning

confidence: 80%

Role of Escherichia coli FtsN protein in the assembly and stability of the cell division ring

Rico

García-Ovalle

Palacios

et al. 2010

Molecular Microbiology

View full text Add to dashboard Cite

SummaryDeprivation of FtsN, the last protein in the hierarchy of divisome assembly, causes the disassembly of other elements from the division ring, even extending to already assembled proto-ring proteins. Therefore the stability and function of the divisome to produce rings active in septation is not guaranteed until FtsN is recruited. Disassembly follows an inverse sequential pathway relative to assembly. In the absence of FtsN, the frequencies of FtsN and FtsQ rings are affected similarly. Among the proto-ring components, ZipA are more sensitive than FtsZ or FtsA rings. In contrast, removal of FtsZ leads to an almost simultaneous disappearance of the other elements from rings. Although restoration of FtsN allows for a quick reincorporation of ZipA into proto-rings, the de novo joint assembly of the three components when FtsZ levels are restored to FtsZ-deprived filaments is even faster. This suggests that the recruitment of ZipA into FtsZ-FtsA incomplete proto-rings may require first a period for the reversal of these partial assemblies.

show abstract

Section: Polyclonal Antiseramentioning

confidence: 80%

Role of Escherichia coli FtsN protein in the assembly and stability of the cell division ring

Rico

García-Ovalle

Palacios

et al. 2010

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…(ui) Septation mutants. The kinetics of growth and division of the various fts mutants have been reported earlier (2,30). A brief summary of relevant observations is given here for ease of reference.…”

Section: Resultsmentioning

confidence: 94%

Cell shape and division in Escherichia coli: experiments with shape and division mutants

Begg¹,

Donachie²

1985

J Bacteriol

Self Cite

206

View full text Add to dashboard Cite

Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsl, ftsQ, or ftsZ) required for septation were constructed. Such mutants were able to grow for about two mass doublings at a normal rate at the restrictive temperature (42°C). The morphology of the cells formed under these conditions was interpreted by assuming the existence of a generalized system for peptidoglycan growth together with two additional systems which modify the shape of the growing peptidoglycan layer. The results also showed that differentfts genes probably control different stages in septation. ftsZ (suUB or sfiB) appears to be required for the earliest step in septation, ftsQ and ftsl (pbpB or sep) are required for a later step or steps, and ftsA is required only for the latest stages in septation.

show abstract

“…The best characterized of this group, encoded by the pbpB gene, is the PBP3 protein (PBP 2B in B. subtilis; Yanouri et al, 1993;Daniel et al, 1996), which is a penicillin-binding protein responsible for cross-linking of the peptidoglycan specifically in the cross wall (Spratt, 1977). FtsQ (DivIB in B. subtilis) is weakly conserved, and its function is not yet known (Begg et al, 1980;Harry et al, 1993;Harry and Wake, 1997). The other proteins in this category are more poorly conserved, with E. coli proteins FtsN (Dai et al, 1993) and ZipA (Hale and De Boer, 1997) having no clear homologues in B. subtilis, and DivIC of B. subtilis (Levin and Losick, 1994;Katis et al, 1997) no obvious counterpart in E. coli.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the essential cell division gene ftsL (yllD ) of Bacillus subtilis and its role in the assembly of the division apparatus

Daniel

Harry

Katis

et al. 1998

Molecular Microbiology

110

138

View full text Add to dashboard Cite

SummaryWe have identified the Bacillus subtilis homologue of the essential cell division gene, ftsL, of Escherichia coli. Repression of ftsL in a strain engineered to carry a conditional promoter results in cell filamentation, with a near immediate arrest of cell division. The filaments show no sign of invagination, indicating that division is blocked at an early stage. FtsL is also shown to be required for septation during sporulation, and depletion of FtsL blocks the activation but not the synthesis of the prespore-specific sigma factor, F . Immunofluorescence microscopy shows that depletion of FtsL has little or no effect on FtsZ ring formation, but the assembly of other division proteins, DivIB and DivIC, at the site of division is prevented. Repression of FtsL also results in a rapid loss of DivIC protein, indicating that DivIC stability is dependent on the presence of FtsL, in turn suggesting that FtsL is intrinsically unstable. The instability of one or more components of the division apparatus may be important for the cyclic assembly/disassembly of the division apparatus.

show abstract

Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell division gene ftsQ

Cited by 93 publications

References 14 publications

Role of Escherichia coli FtsN protein in the assembly and stability of the cell division ring

Role of Escherichia coli FtsN protein in the assembly and stability of the cell division ring

Cell shape and division in Escherichia coli: experiments with shape and division mutants

Characterization of the essential cell division gene ftsL (yllD ) of Bacillus subtilis and its role in the assembly of the division apparatus

Contact Info

Product

Resources

About