Identification of new sources of tomato resistance to A. alternata strains

Nekoval, S. N.; Zakharchenko, Anastasia; Sadovaya, Anastasia; Churikova, Arina; Fedoryanskaya, Irina

doi:10.53859/02352451_2021_35_9_31

Cited by 1 publication

(2 citation statements)

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“…The use of DNA marking technologies in the breeding paradigm significantly accelerates development of modern genotypes with desired properties, their introduction to the market, increasing competitiveness and reducing import dependence on foreign companies. At the first stage of the breeding program for development of tomato genotypes with increased immunity to P. infestans, we selected variety samples contrasting in terms of resistance to late blight, namely, the differentiator variety Ottawa 30 (for the tomato late blight race T1) [1,17] and susceptible line 4, as well as samples with positive morphological and biological characteristics and resistance to other dominant diseases in the south of Russia (fusarium and tomato spotted wilt virus). Their DNA polymorphism was studied using microsatellite molecular markers.…”

Section: Resultsmentioning

confidence: 99%

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Molecular research in the tomato breeding for resistance to biotic stressors

Ovod¹,

Dubina

Garkusha³

2022

BIO Web Conf.

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An urgent problem in tomato breeding is the development of universal varieties and hybrids that combine high yields and good fruit quality with group resistance to a number of diseases. The purpose of this study is to develop tomato breeding material resistant to biotic stressors, basing on modern postgenomic technologies using molecular markers. At the initial stage, we tested microsatellite markers from foreign literature sources, the primers used in the study were synthesized by Syntol LLC, samples from the collection of FSBSI Federal Scientific Center for Biological Plant Protection and FSBSI Federal Scientific Rice Centre were studied under the conditions of Krasnodar region. PCR conditions for identifying the target Ph -3 gene in the tomato plant material used in the breeding process were optimized. Thus, in our study on the testing of microsatellite markers to identify the target Ph-3 resistance gene in the tomato genetic material, we selected 3 informative microsatellite markers R2M1S, G8-1, R1-3U, which reveal the allelic difference between susceptible and resistant samples.

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Section: Resultsmentioning

confidence: 99%

“…), etc. [1]. Field studies conducted in the central soil-climatic zone of Krasnodar region on the territory of experimental plot of FSBSI All-Russian Rice Research Institute, in the period from 2010 to 2021, showed that the most epiphytotic in terms of the 2012,2013,2016,2021.…”

Section: Introductionmentioning

confidence: 99%