Identification of new spectral signatures associated with dengue virus infected sera

Abstract

In a quest to use Raman spectroscopy as an optical diagnostic tool, we recorded Raman spectra of 32 dengue virus (DENV)-infected and 28 healthy sera samples in the near-infrared spectral range (540 to 1700 cm 1 ) using laser at 785 nm as the excitation source. We observed clear differences in the Raman spectra of DENV-infected sera as compared with those of healthy individuals. Here, as a result of our study, we report 12 unique Raman bands associated with DENV-infected sera that are not reported earlier. After applying analysis of variance and t-test (p < 0.05) on these 12 bands, six Raman bands at 630 (N-acetylglucosamine), 883 (in-plane bending (ring) of deoxyribose), 1218 (amide III-β conformation from C 6 H 5 -C stretching vibrations of tryptophan and phenylalanine), 1273 (amide-III), 1623 (tryptophan) and 1672 cm 1 (ceramide) were found only in the DENV-infected sera. The remaining six Raman bands at 716 (lipids), 780 (Uracil-based ring breathing mode), 828 (ring breathing tyrosine), 840 (α-anomers), 1101 (ν(C-N) of lipids and DNA) and 1150 cm 1 (glycogen/carotenoids) were only found in healthy sera. Two types of classification models, principal component analysis and linear discriminant analysis, were employed to develop principal component analysis-linear discriminant analysis model that has provided diagnostic accuracy 96.50%, sensitivity 93.44%, and specificity 100%. This indicates that these 12 Raman bands have the potential to be used as biomarkers for optical diagnosis of DENV infection. This study provides a new insight for future research in the field of optical diagnosis using Raman spectroscopy.

Optical diagnosis of hepatitis B virus infection in blood plasma using Raman spectroscopy and chemometric techniques

“…Raman spectra of healthy and diseased blood plasma samples were acquired directly from the sample surface by placing its quantity of ~20 μL in a semispherical cavity of 5 mm in diameter and 5 mm deep, which was made on the surface of clean aluminum block. This sample holder was used because being metal, it does not produce Raman scattering and it radiates any heat if produced due to the focusing of laser on the sample 16 . The sample was placed under the microscope RSM‐785 coupled with Raman system Peak Seeker PRO‐785 (Agiltron, USA) for the acquisition of spectra.…”

Heating Effects of Desi Ghee Using Raman Spectroscopy

“…From the sample surface, ten Raman spectra were recorded by shining a laser power of 80 mW for an integration time of 10 s. Laser power was optimized carefully to avoid photodegradation. 31,32 Laser power was optimized in our last publication for serum samples. 31 Raman spectra were recorded with four laser powers at 40, 60, 80, and 100 mW.…”

“…31,32 Laser power was optimized in our last publication for serum samples. 31 Raman spectra were recorded with four laser powers at 40, 60, 80, and 100 mW. These spectra were found to be completely overlapping in spectral bands and linearly rising in intensity.…”

Recent advances in linear and nonlinear Raman spectroscopy. Part XII

“…They reported 12 unique Raman bands associated with DENV‐infected sera that were not reported earlier. They concluded that these 12 Raman bands have the potential to be used as biomarkers for optical diagnosis of DENV infection . Antwi‐Boasiako et al described a bioconjugated graphene oxide‐based Raman probe for selective identification of SKBR3 breast cancer cells.…”

“…They concluded that these 12 Raman bands have the potential to be used as biomarkers for optical diagnosis of DENV infection. [59] Antwi-Boasiako et al described a bioconjugated graphene oxide-based Raman probe for selective identification of SKBR3 breast cancer cells. Their experimental results open up a possibility of new label-free Raman scattering assays for the reliable diagnosis of cancer cell lines by monitoring the "turn-off" of the Raman signal from a bio-graphene-oxide nanostructure.…”