2019
DOI: 10.1080/19491034.2019.1618175
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Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cells

Abstract: The double membrane nuclear envelope (NE), which is contiguous with the ER, contains nuclear pore complexes (NPCs) – the channels for nucleocytoplasmic transport, and the nuclear lamina (NL) – a scaffold for NE and chromatin organization. Since numerous human diseases linked to NE proteins occur in mesenchyme-derived cells, we used proteomics to characterize NE and other subcellular fractions isolated from mesenchymal stem cells and from adipocytes and myocytes. Based on spectral abundance, we calculated enric… Show more

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Cited by 47 publications
(53 citation statements)
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“…Slc22a32 (previously named Mfsd10 before inclusion into the Slc22 family) was also one of the strongly regulated targets in our study and has an unknown function but a structure that resembles that of other transporters. Recently, Mfsd10 has been identified as a protein expressed at the nuclear envelope, possibly involved in transport of toxic material out from the nuclear environment (Cheng et al, 2019). In our experiments, Slc22a32 (Mfsd10) was downregulated during glucose starvation in cortex cells, and in starved adult flies, while upregulated during glucose deprivation in both cells and flies.…”
Section: Discussionsupporting
confidence: 49%
“…Slc22a32 (previously named Mfsd10 before inclusion into the Slc22 family) was also one of the strongly regulated targets in our study and has an unknown function but a structure that resembles that of other transporters. Recently, Mfsd10 has been identified as a protein expressed at the nuclear envelope, possibly involved in transport of toxic material out from the nuclear environment (Cheng et al, 2019). In our experiments, Slc22a32 (Mfsd10) was downregulated during glucose starvation in cortex cells, and in starved adult flies, while upregulated during glucose deprivation in both cells and flies.…”
Section: Discussionsupporting
confidence: 49%
“…The strongest of these interactions was with Sur2, a conserved sphingolipid metabolic enzyme with bi-functionality as a sphingosine hydroxylase and ∆4-desaturase ( Haak et al 1997 ; Sperling et al 2001 ; Nakase et al 2010 ; Vacchina et al 2012 ). While a functional role for Sur2 at the INM remains to be explored in fission yeast, Sur2 was found to have access to the INM in S. cerevisiae ( Smoyer et al 2016 ), and the mammalian sphingolipid hydroxylase Smpd4 is enriched at the NE and physically interacts with components of the NPC ( Cheng et al 2019 ; Magini et al 2019 ). Further, Sur2 substrates, including the long-chain base dihydrosphingosine, are enriched at the nuclear membrane where they play a key role in maintaining nuclear morphology in both yeast and mammalian cells ( Hwang et al 2019 ).…”
Section: Resultsmentioning
confidence: 99%
“…The strongest of these interactions was with Sur2, a conserved sphingolipid metabolic enzyme with bi-functionality as a sphingosine hydroxylase and Δ 4-desaturase (7275). While a functional role for Sur2 at the INM remains to be explored in fission yeast, Sur2 was found to have access to the INM in S. cerevisiae (18), and the mam-malian sphingolipid hydroxylase Smpd4 is enriched at the NE and physically interacts with components of the NPC (76, 77). Further, Sur2 substrates, including the long-chain base dihydrosphingosine, are enriched at the nuclear mem-brane where they play a key role in maintaining nuclear morphology in both yeast and mammalian cells (78).…”
Section: Resultsmentioning
confidence: 99%