Identification of nitric oxide in mitochondria of myometrium cell

Abstract

Aim. To demonstrate the possibility of NO synthesis in intact myocytes of uterus. Methods. Confocal scanning microscopy method, NO-sensitive fl uorescent probe DAF-FM, MitoTracker Orange CM-H 2 T-MRos. Results. The basal production of NO in intact myocytes was shown using DAF-FM. Incubation of myocytes with NO donor -sodium nitroprusside (SNP) -led to an increase of the DAF-FM-T fl uorescent signal. On the contrary, the addition of NO-synthase inhibitor -N-nitro-L-arginine (NA) -results in the reduction of fl uorescent intensity. It was demonstrated colocalizition of specifi c probe for mitochondria MitoTracker Orange CM-H 2 TMRos and NO-sensitive dye DAF-FM. Conclusions. For the fi rst time it has been demonstrated the presence of NO in smooth muscle cell mitochondria using laser confocal microscopy, NO-sensitive probe DAF-FM and specifi c marker of the functionally active mitochondria MitoTracker Orange CM-H 2 TMRos. K e y w o r d s: nitric oxide, myometrium, DAF-FM, confocal microscopy.