Identification of nitric oxide (NO)-responsive genes under hypoxia in tomato (Solanum lycopersicum L.) root

Abstract

Flooding periods, as one probable consequence of climate change, will lead more frequently to plant hypoxic stress. Hypoxia sensing and signaling in the root, as the first organ encountering low oxygen, is therefore crucial for plant survival under flooding. Nitric oxide has been shown to be one of the main players involved in hypoxia signaling through the regulation of ERFVII transcription factors stability. Using SNP as NO donor, we investigated the NO-responsive genes, which showed a significant response to hypoxia. We identified 395 genes being differentially regulated under both hypoxia and SNP-treatment. Among them, 251 genes showed up- or down-regulation under both conditions which were used for further biological analysis. Functional classification of these genes showed that they belong to different biological categories such as primary carbon and nitrogen metabolism (e.g. glycolysis, fermentation, protein and amino acid metabolism), nutrient and metabolites transport, redox homeostasis, hormone metabolism, regulation of transcription as well as response to biotic and abiotic stresses. Our data shed light on the NO-mediated gene expression modulation under hypoxia and provides potential targets playing a role in hypoxia tolerance. These genes are interesting candidates for further investigating their role in hypoxia signaling and survival.

Nitric oxide (NO) and salicylic acid (SA): A framework for their relationship in plant development under abiotic stress

“…Although the enzymatic origin is still debated (Corpas & Barroso, 2017; Kolbert et al, 2019), there is no doubt that NO participates in almost all plant physiological processes, including the mechanism of response to adverse environmental conditions. Thus, RNS can modulate gene (González‐Gordo et al, 2019; Safavi‐Rizi et al, 2020) but also protein functions through post‐translational modifications, mainly S ‐nitrosation and tyrosine nitration (Corpas et al, 2013b; Huang et al, 2019; Gupta et al, 2020).…”

Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space

“…Solyc02g062510.3.1, which shares 86% homology with the S. tuberosum Peroxidase 72-like gene, and Solyc02g077300.2.1 (tomato Peroxidase 19) are involved in the phenylpropanoids biosynthesis ( Mei et al, 2009 ). In addition, also Solyc04g080760.3.1 (tomato Peroxidase 9) plays a role in hypoxia tolerance and maintaining the iron balance in tomato ( Safavi-Rizi et al, 2020 ). Peroxidase 9 was also demonstrated to be a pathogenesis-related protein upregulated upon Tomato Mosaic Virus infection ( Andolfo et al, 2014 ).…”

How Extracellular Reactive Oxygen Species Reach Their Intracellular Targets in Plants

“…Nitric oxide (NO • ), a highly reactive small gaseous molecule, modulates the expression of several genes involved in hormonal signaling, primary metabolism, and stress responses ( Palmieri et al, 2008 ; Safavi-Rizi et al, 2020 ). In addition to cytosolic NO • , various physiological functions of apoplastic NO • have been reported ( Besson-Bard et al, 2008 ; Bethke et al, 2004 ; StoÈhr and Ullrich, 2002 ).…”