2015
DOI: 10.1186/s12014-015-9078-5
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Identification of Nocardia species using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry

Abstract: BackgroundThe MALDI (matrix-assisted laser desorption/ionization) Biotyper system for bacterial identification has already been utilized in clinical microbiology laboratories as a successful clinical application of protoemics. However, in cases of Nocardia, mass spectra suitable for MALDI Biotyper identification are often not obtained if such specimens are processed like general bacteria. This problem is related to the insufficiencies in bacterial spectrum databases that preclude accurate specimen identificati… Show more

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Cited by 29 publications
(24 citation statements)
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“…Due to its high discriminatory power and accuracy, multilocus sequence analysis has replaced DNA-DNA hybridization in taxonomy and speciation and for the study of intrageneric relationships (Shneyer, 2007). In spite of the availability of many other molecular methods such as PCR restriction enzyme pattern analysis (Rodríguez-Nava et al, 2006), PCR-RFLP (Conville et al, 2000;Steingrube et al, 1995Steingrube et al, , 1997 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Segawa et al, 2015), gene sequencing remains the best method of identification of Nocardia spp. (McTaggart et al, 2010).…”
Section: Discussionmentioning
confidence: 99%
“…Due to its high discriminatory power and accuracy, multilocus sequence analysis has replaced DNA-DNA hybridization in taxonomy and speciation and for the study of intrageneric relationships (Shneyer, 2007). In spite of the availability of many other molecular methods such as PCR restriction enzyme pattern analysis (Rodríguez-Nava et al, 2006), PCR-RFLP (Conville et al, 2000;Steingrube et al, 1995Steingrube et al, , 1997 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Segawa et al, 2015), gene sequencing remains the best method of identification of Nocardia spp. (McTaggart et al, 2010).…”
Section: Discussionmentioning
confidence: 99%
“…MALDI-TOF MS is a rapid mass spectrometry technology developed in the late 1980s with relatively high sensitivity to various samples types. It is a useful, fast and accurate tool for routine laboratory analysis and has been used to identify mycobacteria [8,9], nocardia [10], yeasts [11,12] and anaerobes [13,14] isolated from solid media of clinical specimens. At present, there are few studies to evaluate the efficacy of identifying anaerobic bacteria by MALDI-TOF MS.…”
Section: Introductionmentioning
confidence: 99%
“…bead-beating) as well as the development of custom databases improved the species-level identification rates of Nocardia spp. (79-90% correctly identified) (Segawa et al, 2015;Verroken et al, 2010).…”
Section: Identification Of Gram-positive Bacteriamentioning
confidence: 89%