Identification of non-volatile and volatile organic compounds produced by Bacillus siamensis LZ88 and their antifungal activity against Alternaria alternata

Wang, Dongkun; Li, Yichi; Yuan, Yuan; Chu, Depeng; Cao, Jianmin; Sun, Guangjun; Ai, Yongfeng; Cui, Zhiyan; Zhang, Yongfeng; Wang, Fenglong; Wang, Xiaoqiang

doi:10.1016/j.biocontrol.2022.104901

Cited by 29 publications

(21 citation statements)

References 45 publications

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“…This antifungal 3-methylbutanoic acid or commonly isovaleric acid is a branched-chain alkyl carboxylic acid and is classified as a short-chain fatty acid with high solubility in water [39]. Such properties could enable its adsorption to the surface membrane, resulting in the alteration of membrane properties and compromising the membrane-associated functions [39,43]. Other related fatty acid derivatives have been hypothesized to cause antifungal activity by penetrating and disrupting the lipid bilayer of fungal cell membranes, increasing membrane fluidity, and causing membrane disintegration and the collapsing of cells [54].…”

Section: Discussionmentioning

confidence: 99%

“…It is plausible that 3-methylbutanoic acid (as a short-chain fatty acid) could cause such effects in the target fungal cells depending on the degree of similarities in the function group and the aliphatic chain length. The antifungal activity of 3-methylbutanoic acid has been demonstrated against various phytopathogens, including Fusarium spp., Pyricularia oryzae, Rhizoctonia cerealis, Alternaria alternata, Phytophthora parasitica, Cladosporium cladosporioides, Penicillium expansum, Aspergillus spp., Penicillium spp., Rhizopus spp., Mucor spp., and Botryotinia fuckeliana [39,43,55]. The commonly exhibited antifungal effects of 3-methylbutanoic acid include the reduction in the rate of mycelial growth (reduced mycelial density), reducing the rate of spore germination, and the suppression of germ tube elongation [39,43,55,56].…”

Section: Discussionmentioning

confidence: 99%

“…The antifungal activity of 3-methylbutanoic acid has been demonstrated against various phytopathogens, including Fusarium spp., Pyricularia oryzae, Rhizoctonia cerealis, Alternaria alternata, Phytophthora parasitica, Cladosporium cladosporioides, Penicillium expansum, Aspergillus spp., Penicillium spp., Rhizopus spp., Mucor spp., and Botryotinia fuckeliana [39,43,55]. The commonly exhibited antifungal effects of 3-methylbutanoic acid include the reduction in the rate of mycelial growth (reduced mycelial density), reducing the rate of spore germination, and the suppression of germ tube elongation [39,43,55,56]. Despite the comparatively low relative peak area of 2.92% in the headspace composition, 3-methylbutanoic acid could be a vital component for the antifungal activity of the VOCs produced by B. velezensis CE 100.…”

Section: Discussionmentioning

confidence: 99%

“…In their study, the main VOCs that were detected using HS-SPME/CG-MS include pyrazine (2,5-dimethyl), benzothiazole, 4-chloro-3-methyl, and phenol-2,4-bis (1,1-dimethyl ethyl) [41]. Some of the VOCs that are notably known for antifungal activity against plant pathogens include primary amines [42], and organic acids such as 3-methyl butanoic acid and 2-methylbutanoic acid [39,43]. These antifungal VOCs can disrupt the hyphal morphology and lowers the cell membrane stability, which causes desiccation and cell invasion by water-soluble toxins, due to increased cell membrane permeability [33].…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Activity of Volatile Organic Compounds from Bacillus velezensis CE 100 against Colletotrichum gloeosporioides

et al. 2022

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Colletotrichum gloeosporioides is the most prevalent phytopathogen, causing anthracnose disease that severely affects the production of various fruit trees, including walnut and jujube. In this study, the volatile organic compounds (VOCs) from Bacillus velezensis CE 100 disrupted the cell membrane integrity of C. gloeosporioides and reduced the spore germination by 36.4% and mycelial growth by 20.0% at a bacterial broth concentration of 10%, while the control group showed no antifungal effect. Based on the headspace solid-phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) analysis, seven VOCs were identified from the headspace of B. velezensis CE 100. Out of the seven VOCs, 5-nonylamine and 3-methylbutanoic acid were only detected in the headspace of B. velezensis CE 100 but not in the control group. Both 5-nonylamine and 3-methylbutanoic acid showed significant antifungal activity against the spore germination and mycelial growth of C. gloeosporioides. Treatment with 100 µL/mL of 5-nonylamine and 3-methylbutanoic acid suppressed the spore germination of C. gloeosporioides by 10.9% and 30.4% and reduced mycelial growth by 14.0% and 22.6%, respectively. Therefore, 5-nonylamine and 3-methylbutanoic acid are the potential antifungal VOCs emitted by B. velezensis CE 100, and this is the first report about the antifungal activity of 5-nonylamine against C. gloeosporioides.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antifungal Activity of Volatile Organic Compounds from Bacillus velezensis CE 100 against Colletotrichum gloeosporioides

et al. 2022

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“…Volatile substances produced by Bacillus spp. reportedly inhibit phytopathogenic fungi ( Li et al, 2020 ; De la Cruz-López et al, 2022 ; Wang et al, 2022 ), affect soil microbial richness ( Yuan et al, 2017 ) and promote plant growth ( Ghazala et al, 2022 ; Rojas-Sanchez et al, 2022 ). Nevertheless, the mechanisms by which the strain and its volatile substances affect pathogenic fungi in vivo require further study.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Chen

Zhang

et al. 2022

Front. Microbiol.

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Soft rot causes significant economic losses in the kiwifruit industry. This study isolated strain CTXW 7-6-2 from healthy kiwifruit tissue; this was a gram-positive bacterium that produced the red pigment pulcherrimin. The phylogenetic tree based on 16S ribosomal RNA, gyrA, rpoB, and purH gene sequences identified CTXW 7-6-2 as a strain of Bacillus subtilis. CTXW 7-6-2 inhibited hyphal growth of pathogenic fungi that cause kiwifruit soft rot, namely, Botryosphaeria dothidea, Phomopsis sp., and Alternaria alternata, by 81.76, 69.80, and 32.03%, respectively. CTXW 7-6-2 caused the hyphal surface to become swollen and deformed. Volatile compounds (VOC) produced by the strain inhibited the growth of A. alternata and Phomopsis sp. by 65.74 and 54.78%, respectively. Whole-genome sequencing revealed that CTXW 7-6-2 possessed a single circular chromosome of 4,221,676 bp that contained 4,428 protein-coding genes, with a guanine and cytosine (GC) content of 43.41%. Gene functions were annotated using the National Center for Biotechnology Information (NCBI) non-redundant protein, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups of proteins, Gene Ontology, Pathogen–Host Interactions, Carbohydrate-Active enZYmes, and Rapid Annotations using Subsystem Technology databases, revealing non-ribosomal pathways associated with antifungal mechanisms, biofilm formation, chemotactic motility, VOC 3-hydroxy-2-butanone, cell wall-associated enzymes, and synthesis of various secondary metabolites. antiSMASH analysis predicted that CTXW 7-6-2 can produce the active substances bacillaene, bacillibactin, subtilosin A, bacilysin, and luminmide and has four gene clusters of unknown function. Quantitative real-time PCR (qRT-PCR) analysis verified that yvmC and cypX, key genes involved in the production of pulcherrimin, were highly expressed in CTXW 7-6-2. This study elucidates the mechanism by which B. subtilis strain CTXW 7-6-2 inhibits pathogenic fungi that cause kiwifruit soft rot, suggesting the benefit of further studying its antifungal active substances.

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Molecular characterization of Aureobasidium spp. strains isolated during the cold season. A preliminary efficacy evaluation as novel potential biocontrol agents against postharvest pathogens

et al. 2023

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Several Aureobasidium spp. strains isolated from wild environments during winter 2022 were characterized by sequence analysis of the internal transcribed spacer (ITS), the translation elongation factor EF-1α gene (EF1), and part of the elongase gene (ELO). The variability in the EF1 and ELO loci are higher than in the ITS. All strains but one (UC14), were identified as A. pullulans. To assess the effectiveness of the characterized strains as biocontrol agents (BCAs) of diseases occurring during postharvest storage, a selection of the strains was evaluated by in vitro and in vivo assays. On average, the reduction of Monilinia spp. colony growth was more marked for non-volatile metabolites than for volatile (VOCs). Strain UC14 provided the strongest mycelial growth reduction of Monilinia fructicola by VOCs (66%). According to the in vivo results, all strains were effective in controlling brown rot during cold storage and remarkably in restricting the growth of Monilinia polystroma. In particular, VB23 was the most effective in controlling brown rot incidence, by 80%, 60%, 100%, and severity, by 79.5%, 72.7% and 100%, for Monilinia laxa, M. fructicola, and M. polystroma, respectively.

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Identification of non-volatile and volatile organic compounds produced by Bacillus siamensis LZ88 and their antifungal activity against Alternaria alternata

Cited by 29 publications

References 45 publications

Antifungal Activity of Volatile Organic Compounds from Bacillus velezensis CE 100 against Colletotrichum gloeosporioides

Antifungal Activity of Volatile Organic Compounds from Bacillus velezensis CE 100 against Colletotrichum gloeosporioides

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Molecular characterization of Aureobasidium spp. strains isolated during the cold season. A preliminary efficacy evaluation as novel potential biocontrol agents against postharvest pathogens

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