Identification of non‐zein proteins in BR473 maize protein bodies by LC‐nanoESI‐MS/MS

Bicudo, Rogério Campos; Bicudo, Tatiana Campos; Forato, L. A.; Titato, Guilherme M.; Colnago, Luiz Alberto; Lanças, Fernando Mauro

doi:10.1002/jssc.200900339

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…Proteomics technologies have made possible a broad survey of all the functional categories of proteins, revealing their differential expression patterns in pathological tissue from various diseases as well as response to treatment [4]. Proteomic analysis coupled with liquid chromatography-nanoelectrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) is a well-established technique for peptide sequencing analysis [5]. The objective of this work was to search for potential targets of RMR treatment in MCF-7 breast cancer cells through a proteomic approach using two-dimensional gel electrophoresis and LC-nanoESI-MS/MS for protein identification.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Human Breast Cancer Cells Treated with <i>Monascus</i>-Fermented Red Mold Rice Extracts

Lee¹,

Hsieh²,

Wu³

et al. 2015

JFNR

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Monascus-fermented red mold rice (RMR) extracts have recently been proved to possess anticancer activities, but the anticancer effect of breast cancer cells treated with RMR is not yet well understood. In an effort to identify proteins that may be involved in RMR-induced breast cancer cell death, we employed proteomic analysis coupled with LC-nanoESI-MS/MS on MCF-7 cells exposed to two RMR extracts, ethyl acetate extract (EAE) and ethanol extract (EE). Sixupregulated proteins were identified in EAE-induced MCF-7 cells, including glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase E1 component, heat shock 27 kDa protein 1, cathepsin D, protein disulphideisomerase A3, and prohibitin. The upregulated expression of other 4 proteins was seen in EE-induced MCF-7 cells, including alloalbuminvenezia, annexin A5, endoplasmic reticulum protein 29 isoform 1 precursor, and cellular retinoic acid binding protein 2. The same two down-regulated proteins were identified on cells after treatment with both extracts, dermcidin preproprotein and poly(rC) binding protein 1. These proteins have been implicated in proapoptotic regulation, stress modulation, tumor suppression, and survival factor activity. These analysescould provide valuable information for further exploration of RMR as a promising chemopreventive agent against breast cancer.

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Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Human Breast Cancer Cells Treated with <i>Monascus</i>-Fermented Red Mold Rice Extracts

Lee¹,

Hsieh²,

Wu³

et al. 2015

JFNR

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Current literature in mass spectrometry

2010

J. Mass Spectrom.

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Rapid measurements of curcumin from complex samples coupled with magnetic biocompatibility molecularly imprinted polymer using electrochemical detection

Jiang

Tan

et al. 2020

J of Separation Science

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Curcumin widely exists in food, and rapid selective and accurate detection of curcumin have great significance in chemical industry. In this experiment, a new magnetic biocompatibility molecularly imprinted polymer was prepared with nontoxic and biocompatible Zein to adsorb curcumin selectively. The polymer has high biocompatibility, good adsorption capacity, and specific adsorption for curcumin. Combined with portable electrochemical workstations, the polymer can be used to detect curcumin rapidly and cost‐effectively. Using curcumin as a template and Zein as the crosslinking agent, the polymers were synthesized on the surface of Fe3O4 particles for solid phase extraction. The experimental results showed that the polymer reached large adsorption capacity (32.12 mg/g) with fast kinetics (20 min). The adsorption characteristic of the polymer followed the Langmuir isotherm and pseudo‐second‐order kinetic models. Hexacyanoferrate was used as electrochemical probe to generate signals, and the linear range was 5–200 µg/mL for measuring curcumin. The experimental analysis showed that the polymer was an ideal material for selective accumulation of curcumin from complex samples. This approach has been successfully applied to the determination of curcumin in food samples with electrochemical detection, indicating that this is a feasible and practical technique.

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Identification of non‐zein proteins in BR473 maize protein bodies by LC‐nanoESI‐MS/MS

Cited by 5 publications

References 32 publications

Proteomic Analysis of Human Breast Cancer Cells Treated with <i>Monascus</i>-Fermented Red Mold Rice Extracts

Proteomic Analysis of Human Breast Cancer Cells Treated with <i>Monascus</i>-Fermented Red Mold Rice Extracts

Current literature in mass spectrometry

Rapid measurements of curcumin from complex samples coupled with magnetic biocompatibility molecularly imprinted polymer using electrochemical detection

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