Identification of Novel Bioactive Compound Derived from Rheum officinalis against Campylobacter jejuni NCTC11168

Abstract

<jats:p>Gastric diseases are increasing with the infection of <jats:italic>Campylobacter jejuni.</jats:italic> Late stages of infection lead to peptic ulcer and gastric carcinoma. <jats:italic>C. jejuni</jats:italic> infects people within different stages of their life, especially childhood, causing severe diarrhea; it infects around two-thirds of the world population. Due to bacterial resistance against standard antibiotic, a new strategy is needed to impede <jats:italic>Campylobacter</jats:italic> infections. Plants provide highly varied structures with antimicrobial use which are unlikely to be synthesized in laboratories. A special feature of higher plants is their ability to produce a great number of organic chemicals of high structural diversity, the so-called secondary metabolites. Twenty plants were screened to detect their antibacterial activities. Screening results showed that <jats:italic>Rheum officinalis</jats:italic> was the most efficient against <jats:italic>C. jejuni</jats:italic>. Fractionation pattern was obtained by column chromatography, while the purity test was done by thin-layer chromatography (TLC). The chemical composition of bioactive compound was characterized using GC-MS, nuclear magnetic resonance, and infrared analysis. Minimal inhibitory concentration (MIC) of the purified compound was 31.25 <jats:italic>µ</jats:italic>g/ml. Cytotoxicity assay on Vero cells was evaluated to be 497 <jats:italic>µ</jats:italic>g/ml. Furthermore, the purified bioactive compound activated human lymphocytes <jats:italic>in vitro</jats:italic>. The data presented here show that <jats:italic>Rheum officinalis</jats:italic> could potentially be used in modern applications aimed at the treatment or prevention of foodborne diseases.</jats:p>

In vitro investigation of antifungal impact of various plant extracts

“…Various plants used in this study were seen in table (1), Furthermore, different plant fungal isolates were identified at (RCMB) Al-Azhar University. The most fungal isolates have been affected with different plant extracts were Aspergillus niger, Penicillium citrinium (B) and Aspergillus fumigatus as shown in (Fig.…”

“…The ability to isolate and identify these phytopathogens is an essential step to investigate and treat these pathogens. During different forms of microbial infection, precise investigation and using combined drugs are recently used for a successful control of infection [1]. Despite, the presence of natural and synthetic products to eradicate fungal threats, many dangerous fungal infections are present and threaten human, animals and plants [2].…”

The Medicinal Relevance of Using “Officinale“ and “Officinalis“ as Plant Species Names in Romanian Flora

“…Uses: against constipation. (Constantinescu, 1986) -It exerts strong bactericidal activity against six aquatic pathogens ( Kuo et al, 2020) -Efficient against Campylobacter jejuni (Yosri et al, 2020) yeast brandy is drunk by women against hernia. (Panțu, 1906) Uses: in vascular disorders; in states of asthenia; after heavy physical exertion; after pneumonia; contagious diseases.…”

Antimicrobial Activity and Multi-therapeutic Potential of Salvia officinalis and Mentha longifolia Essential Oils in Leaves

“…Leaf powders were hydrodistillated for 4 hours in the Clevengersystem (Glassco Laboratory Equipment Pvt., Mumbai, India). 20…”