Identification of novel cytochrome P450 1A genes from five marine mammal species

Teramitsu, Ikuko; Yamamoto, Yukio; Chiba, Issei; Iwata, Hisato; Tanabe, Shinsuke; Fujise, Yoshihiro; Kazusaka, Akio; Akahori, Fumiaki; Fujita, Shoichi

doi:10.1016/s0166-445x(00)00113-2

Cited by 41 publications

(24 citation statements)

References 17 publications

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“…CYPlAl has been sequenced from several cetacean species (c. Godard, unpublished data and Teramtsu et al 2000). In humans, CYPlA2 expression is restrcted to liver and olfactory while CYPIAI is expressed in both hepatic and extrahepatic tissues.…”

Section: Cypia In Mammalsmentioning

confidence: 99%

“…CYPlAI has been detected in cetaceans by immunoassays (White et ai. 1994) and cDNA sequencing (Teramtsu et al 2000) while the paralogous CYPlA2 has not. As yet, there is no evidence to support multiple CYPlA genes in cetaceans and based on the specificity of the antibody used in this study, we refer to the protein that we detect in cetaceans as CYPlAI.…”

Section: Introductionmentioning

confidence: 99%

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Cytochrome P450 1A1 and aromatase (CYP19) in cetaceans : enzyme expression and relationship to contaminant exposure

Wilson¹

2003

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Section: Cypia In Mammalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytochrome P450 1A1 and aromatase (CYP19) in cetaceans : enzyme expression and relationship to contaminant exposure

Wilson¹

2003

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“…Total RNA was prepared from each liver sample by the single-step method [2] using TRI reagent from Sigma Chemical Co. (St. Louis, MO, U.S.A.). Degenerate primers, sense (5'-TCT TTG GRG CWG GNT TTG ACA C-3') and anti-sense (5'-TGG TTR AYC TGC CAC TGG TT-3'), were both positioned upstream from the well-described cysteine-containing heme-binding region and used to amplify fragments of CYP1A cDNAs [17]. This region is regarded as an unconverted region of CYP1A1 and CYP1A2 in mammals and suggested to be the appropriate region for analyses of the CYP1A1 sequence.…”

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confidence: 99%

Expression and Sequence of CYP1A1 in the Camel

Darwish

Morshdy

Ikenaka

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. In this study, we determined a partial sequence of CYP1A1 in the camel and its phylogenetic position. The deduced amino acid sequence of camel CYP1A1 showed the highest identity 94% with those of sheep and cattle CYP1A1. In a phylogenetic analysis, the camel CYP1A1 isoform was located beside sheep and cattle CYP1A1. When we studied the distribution of camel CYP1A1 mRNA in different tissues, we found that this isoform was expressed in all tissues except the hump. Interestingly, the lungs of all the camels and tongues of two of the three animals showed high expressions of CYP1A1 mRNA, and this may indicate exposure to ligands of aryl hydrocarbon receptor such as environmental pollutants or flavonoids.

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“…CYP1A nucleotide sequences have been determined in rainbow trout (Berndtson and Chen, 1994;Heilmann et al, 1988), plaice (Leaver et al, 1993), Atlantic tomcod (Roy et al, 1995), toadfish and scup (Morrison et al, 1995), killifish (Morrison et al, 1998), red sea bream (Mizukami et al, 1994), sea bass (Stien et al, 1998) and, more recently, Atlantic salmon (Rees et al, 2003). Current evidence shows that several CYP1A isoforms exist in fish (Teramitsu et al, 2000) and they are distributed in tissues such as the liver, gut, kidney, gill and heart (Goksøyr and Husoy, 1998;Sarasquete and Segner, 2000;Stegeman and Hahn, 1994). Few immunocytochemistry studies show that constitutive CYP1A proteins are present in the neurons and the endothelia of the fish brain (Sarasquete and Segner, 2000).…”

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confidence: 99%

β-naphthoflavone induction of CYP1A in brain of juvenile lake trout(Salvelinus namaycush Walbaum)

Chung-Davidson

Rees

et al. 2004

Journal of Experimental Biology

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Many environmental pollutants induce expression of the cytochrome P450 (CYP) 1A subfamily of genes. We integrated cellular and molecular biological techniques to examine the effects of β-naphthoflavone (BNF) exposure in lake trout brain CYP1A distribution and dynamics. Over a 32-day time-course, real time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) results showed that CYP1A mRNA induction in response to BNF exposure occurred rapidly and continued to rise in the BNF-treated lake trout after 4·h, with a peak at or after 2·days. Messenger RNA levels fell after 4·days, and this trend continued after 16·days of exposure. In situ hybridization indicated that CYP1A mRNA was universally elevated in the brain of BNF-exposed fish and was mainly expressed in the endothelia and occasionally in the glial cells. CYP1A immunoreactivity was induced in the olfactory bulb and valvula cerebelli of BNF-treated fish. Other brain areas showed constitutive CYP1A immunoreactivity in both control and BNF-treated fish. Some BNF-treated fish contained multifocal hemorrhages in the brain tissue, and these fish had overall depressed CYP1A immunoreactivity in the brain. The relationship between transcriptional and translational effects of BNF exposure in the brain of juvenile lake trout is discussed.

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Identification of novel cytochrome P450 1A genes from five marine mammal species

Cited by 41 publications

References 17 publications

Cytochrome P450 1A1 and aromatase (CYP19) in cetaceans : enzyme expression and relationship to contaminant exposure

Cytochrome P450 1A1 and aromatase (CYP19) in cetaceans : enzyme expression and relationship to contaminant exposure

Expression and Sequence of CYP1A1 in the Camel

β-naphthoflavone induction of CYP1A in brain of juvenile lake trout(Salvelinus namaycush Walbaum)

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