Identification of novel genes expressed highly and selectively in the corpora allata of the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Homma, Yuri; Mita, Kazuei; Nakamura, Yuki; Namiki, Toshiki; Noda, Hiroaki; Shinoda, Tetsuro; Togawa, Toru

doi:10.1007/s13355-019-00652-y

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Our work provided evidence for the expression of CP genes in the haemolymph of A. pernyi , where the expression of CP genes is interesting. The CPs synthesized in the non-epidermis cells perhaps make use of them in these organs [ 46 ]. This is supported by a study on B. mori , where a CPT1 gene was detected in the fat body and was proven to participate in silkworm innate immunity via the recognition of Escherichia coli [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi

Chen

Xia

et al. 2023

IJMS

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Antheraea pernyi is one of the most famous edible and silk-producing wild silkworms of Saturniidae. Structural cuticular proteins (CPs) are the primary component of insect cuticle. In this paper, the CPs in the genome of A. pernyi were identified and compared with those of the lepidopteran model species Bombyx mori, and expression patterns were analyzed based on the transcriptomic data from the larval epidermis/integument (epidermis in the following) and some non-epidermis tissues/organs of two silkworm species. A total of 217 CPs was identified in the A. pernyi genome, a comparable number to B. mori (236 CPs), with CPLCP and CPG families being the main contribution to the number difference between two silkworm species. We found more RR-2 genes expressed in the larval epidermis of fifth instar of A. pernyi than B. mori, but less RR-2 genes expressed in the prothoracic gland of A. pernyi than B. mori, which suggests that the hardness difference in the larval epidermis and prothoracic gland between the two species may be caused by the number of RR-2 genes expressed. We also revealed that, in B. mori, the number of CP genes expressed in the corpus allatum and prothoracic gland of fifth instar was higher than that in the larval epidermis. Our work provided an overall framework for functional research into the CP genes of Saturniidae.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi

Chen

Xia

et al. 2023

IJMS

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“…For example, silencing the TcJHAMT gene in the larval stage of Tribolium castaneum resulted in premature larva-pupa metamorphosis [ 19 ]. In particular, the genes encoding enzymes for the mevalonate pathway and the last two steps of the JH branch pathway (farnesoic acid (FA) epoxidase and JHAMT ) have been identified in multiple species [ 10 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Allatostatin C (PISCF/AST) and Juvenile Hormone Acid O-Methyltransferase in Dendroctonus armandi

Sun

Liu

et al. 2022

IJMS

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Allatostatin C (PISCF/AST) is a neuropeptide gene that affects juvenile hormone (JH) synthesis in the corpora allata. Juvenile hormone acid O-methyltransferase (JHAMT) is a key gene in the JH biosynthetic pathway. In this study, two genes encoding DaAST and DaJHAMT were cloned. Both DaAST and DaJHAMT were expressed in the larvae, pupae and adults of Chinese white pine beetle (Dendroctonus armandi), and highly expressed in the head and the gut. The expression of the two genes was induced by JH analog (JHA) methoprene and the functions of the two genes were then investigated by RNAi. Considering the role of hormones in metamorphosis, JHA significantly induced DaAST and DaJHAMT in the larval stage. DaAST knockdown in larvae, pupae and adults significantly increased the DaJHAMT mRNA levels. Moreover, knockdown of DaAST instead of DaJHAMT increased pupae mortality and the abnormal rate of emergence morphology and reduced emergence rates. However, knockdown of DaJHAMT instead of DaAST significantly reduced frontalin biosynthesis in adult males. The results showed that DaAST acts as an allatostatin and inhibits JH biosynthesis, and that JHAMT is a key regulatory enzyme for JH synthesis in the D. armandi.

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A mitochondrial phosphatase PTPMT1 is essential for the early development of silkworm, Bombyx mori

Homma

Toga

Daimon

et al. 2020

Biochemical and Biophysical Research Communications

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Identification of novel genes expressed highly and selectively in the corpora allata of the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Cited by 5 publications

References 37 publications

Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi

Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi

Functional Characterization of Allatostatin C (PISCF/AST) and Juvenile Hormone Acid O-Methyltransferase in Dendroctonus armandi

A mitochondrial phosphatase PTPMT1 is essential for the early development of silkworm, Bombyx mori

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