Identification of novel microsatellite markers for Saraca asoca, a medicinally important tree species in India

Sumangala, R. C.; Shaanker, R. Uma; Dayanandan, Selvadurai; Vasudeva, R.; Ravikanth, G.

doi:10.1007/s12041-013-0277-4

Cited by 8 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ten microsatellite markers of 2-22 alleles per locus identified can be used to study the genetic structure of a population. 62 Randomly Amplified Polymorphic DNA (RAPD) profiling carried out in five natural populations in Orissa, found relatively high genetic variation as well as polymorphism between the populations, thus recommending in situ conservation of the species. 63 Fluorescentlabeled (6-carboxyfluorescein) RAPD primer based study on 6 accessions from Hyderabad, showed more polymorphism, indicating high genetic diversity within the species, even in a small population.…”

Section: (A) Marker Studiesmentioning

confidence: 99%

Saraca asoca– morphology and diversity across its natural distribution in India

Warrier¹

2021

IJCAM

View full text Add to dashboard Cite

Trees play a chief role in the existence of human civilization. We get a glimpse of the trees that have to be planted at a site in Varahamihira's Brihat Samhita. In Brihat Samhita tank bunds are discussed to be shaded with Mangifera indica, Terminalia arjuna, Ficus religiosa, Ficus benghalensis, Syzygium cuminii, Mitragyna parviflora, Borassus flabellifer, Madhuca longifolia, Mimusops elengi, and Saraca asoca 1 .Saraca asoca or Jonesia asoca Roxb., commonly known as Ashoka, is a medicinal tree used to treat gynecological problems, as found in records of Charaka Samhita, Bhavprakasha Nighantu, and the treatise of Susruta 2-4 . Belonging to the genus Saraca, it comes under the Fabaceae family.The genus Saraca includes Saraca asoca (Roxb.) De Wilde, Saraca indica Linné, Saraca thaipingensis Cantley ex Prain, Saraca celebica De Wilde, Saraca griffithiana Prain, Saraca hullettii Prain, Saraca declinata (Jack) Miq., and Saraca dives Pierre, to name a few 5 . Of these, S.asoca (Roxb.) de Wilde, S.indica L., S.declinata Miq. (Red Saraca) and S.thaipingensis Prain (Yellow Saraca) has been reported in India. While S.asoca grows in the wild, the other three are grown in botanical gardens. [6][7] Though mainly used for ornamental purposes, they possess immense medicinal values. 7 Ashoka is a perennial evergreen tree, growing to a height of 10m. 9 Though a native of the Indian sub-continent, it is also seen in Sri Lanka and the Indo-Malaysian region. In India, Ashoka trees are found in Peninsular India, comprising the Western and Eastern Ghats and the sub-Himalayan tracts, to an altitude of 750m. It also prefers to grow in semi-evergreen and moist deciduous forests and is found along river streams. 10,11 Red lateral alluvial soil is suitable for growth and requires an annual rainfall of 2000-4000mm. 12 Saraca asoca (Roxb.) de Wilde, though known as Ashoka, has many common names in different languages -Ashoka in Hindi, Kankeli in Sanskrit, Ashokadamara in Kannada, Ashokapatta in Telugu, Asokam in Malayalam, and Asogam in Tamil. 13 It is considered a 'sacred tree' by both Hindus and Buddhists and holds high significance and importance in mythology, literature, and history. 2 The first variety of Ashoka -Aswani-I -with high bark yield and more tannin content has been released through a single plant selection method. 14 Other than 'Ashokarishta' and 'Ashokaghrita' many tablets, tonics, and syrups made from S.asoca are available in the market like 'Ashotone', and 'Ashonil'. 10 The Ayurvedic formulation of Ashokarishta comprises of Saraca asoca bark, Woodfordia fruticosa flower, Cuminum cyaminum fruit, Cyperus rotundus rhizome, Zingiber officinale rhizome, Berberis aristata stem, Nymphea stellata flower, Terminalia bellirica fruit pericarp, Terminalia chebula fruit pericarp, Phyllanthus emblica fruit pericarp, Mangifera indica endosperm, Adhatoda vasica root, Santalum album heartwood, and jiggery. 15 The bark helps in stopping excessive menstrual bleeding and hemorrhagic dysentery due to the presence of tannins. 16 Chemical investi...

show abstract

Section: (A) Marker Studiesmentioning

confidence: 99%

Saraca asoca– morphology and diversity across its natural distribution in India

Warrier¹

2021

IJCAM

View full text Add to dashboard Cite

show abstract

“…Genetic studies pronounced in S. asoca were specifically limited to resolving identification troubles in crude pills (Urumarudappa et al, 2016;Hegde et al, 2018). However, genetic markers had been used in different research 161 works to evaluate versions, relationships and population genetic parameters in S. asoca (Sumangala et al, 2016). Many phytochemical studies have been performed for the evaluation of various compounds in S. asoca as (+)catechin, (−)-epicatechin, procyanidine B-2, eleven-deoxyprocyanidin B4, leucocyanidin and so on (Shirolkar et al, 2013;Singh et al, 2015).…”

Section: Biotechnological Approaches Towards Improvement Of Saraca Asmentioning

confidence: 99%

“…In a take a look at conducted on improvement of microsatellite markers in S. asoca a complete of 15 microsatellite primer pairs have been synthesized and screened for polymorphism (Sumangala et al 2013). After initial screening of the 15 primers, 10 primer pairs that confirmed polymorphism have been retained for in addition analysis.…”

mentioning

confidence: 99%

Review of Medicinally Important Plant Species Saraca Asoca (Roxb.)

Yadav¹

2019

IJAR

View full text Add to dashboard Cite

Saraca asoca is a relatively traded IUCN crimson listed tree species used in Ayurvedic drug treatments for the treatment of numerous disorders, especially gynecological troubles. However, information approximately the genetic variations among populations and corresponding variation in specialized metabolites of S. asoca stays uncertain. The presence of various antioxidant compounds including ascorbic acid, catechin, flavonoids, lignin glycosides, beta-sitosterol and its glucosidic identified in S. asoca leaf, bark and flower. The present study will provide basic information about distribution of Saraca asoca genotypes in India. Molecular characterization of Saraca asoca using molecular markers will be useful in the identification of diverse genotypes available in the country. Diverse genotypes and highly therapeutic compound may be used for future conservation and improvement program of Saraca asoca. Study has potential scope in the management of Saraca asoca species.

show abstract

“…The within population variation was higher (82%) than among population diversity (18%). Microsatellite markers have also been developed for diversity analysis in this species (Sumangala et al, 2013). Saraca specific SCAR markers with an amplicon length of 193 bp has been developed for identification and discrimination of Saraca from its adulterants (Kumar, 2016).…”

Section: Diversity Studiesmentioning

confidence: 99%

Review article DNA fingerprinting in industrially important medicinal trees

Warrier¹,

Joshi²,

Arunkumar³

2019

View full text Add to dashboard Cite

Herbal medicine has been considered as a promising future medicine for the management of healthcare. The shift "Return back to Nature" has enhanced the consumption of plant-based phytomedicines and other botanicals in recent years. Medicinal plants are considered as rich sources of phytochemical ingredients. With a big gap in the demand and supply, there is heavy exploitation of these resources. The fast depleting are the medici nal trees, which, due to their long gestation, fails to find a place in cultivation. There is a need to encourage cultivation of these species. Traditional breeding and biotechnology can be used in their genetic improvement. Environmental conditions affect the active constituents of medicinal plants and, therefore their activity profiles. Hence, there is a need to understand the geographical variation at the genetic level. DNA-based techniques have been widely used for assessing diversity and authentication of plant species of medicinal importance. This paper describes the demand of some medicinal trees which are in high demand in the Ayurvedic industry, and the application of DNA techniques to understand the geographic distribution, authentication and confirmation of identity of the species.

show abstract

Identification of novel microsatellite markers for Saraca asoca, a medicinally important tree species in India

Cited by 8 publications

References 8 publications

Saraca asoca– morphology and diversity across its natural distribution in India

Saraca asoca– morphology and diversity across its natural distribution in India

Review of Medicinally Important Plant Species Saraca Asoca (Roxb.)

Review article DNA fingerprinting in industrially important medicinal trees

Contact Info

Product

Resources

About