Identification of novel nonedible oil seeds via scanning electron microscopy for biodiesel production

Ullah

et al. 2021

Self Cite

Investigation of alternative energy sources is need of current time due to growing power crisis and associated environmental issues. Biodiesel is considered as sustainable power source and promising alternative to fossil fuels. Therefore, our current investigation aimed to identify micromorphological characters of 10 novel nonedible oil-yielding seeds through scanning electron microscopy. It was revealed from light microscopic study that there is variation in seed size from 3 to 15 mm in length and 2 to 11 mm in width. Likewise, a huge variation in color was observed such as light green, greenish yellow, blackish brown, and various shades of brown. Presence and absence of Hilum was observed, and compression of seeds varied from depressed, lateral, and dorsoventral. Seed's shape differs from ovate, clavate, triangular ovate, cuneiform, ovoid, and elliptical shape. Seed oil content fall in range of 18-58% (wt/wt). Free fatty acid content of the seeds varies from 0.3 to 3.1 mg KOH/g. Ultrastructure of seeds exhibited huge variation in shape, size, periclinal wall, anticlinal wall, and surface ornamentation. Nonedible seeds varied in wall structure from angular, wavy, dentate entire, irregular, puzzled, elongated, even, and polygonal.The periclinal wall arrangements show alteration from flat, looped, raised, depressed, lofty, even, pentagonal, polygonal, and undulate seed margins. Outcomes of this investigation recommended that scanning electron microscopy could act as a helpful tool in disclosing the hidden micromorphological characters among nonedible oilyielding seeds and subsequently helping in correct, authentic seed identification and classification as potential feedstock for biodiesel.

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Microscopic techniques for characterization and authentication of oil‐yielding seeds

Aziz

Ullah

et al. 2021

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“…Measurements were repeatedly taken for three to five times and mean values were recorded. Length and width of sample seeds were measured using a ruler (Aziz et al, 2021; Dawood et al, 2020; Munir et al, 2021). Macromorphological features were further validated from flora of Pakistan.…”

Section: Methodsmentioning

confidence: 99%

Implication of scanning electron microscopy as a tool for identification of novel, nonedible oil seeds for biodiesel production

Rozina

Khan

et al. 2021

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Biodiesel is a promising, bio‐based, renewable, nontoxic, environment friendly, and alternative fuel for petroleum derived fuels which helps to reduce dependency on conventional fossil fuels. In this study, six novel, nonedible seed oil producing feedstock were explored for their potential for sustainable production of biodiesel. It is very important to correctly identify oil yielding plant species. Scanning electron microscopy (SEM) was used as reliable tool for authentic identification of oil yielding seeds. Macromorphological characters of seeds were studied with light microscopy (LM). Outcomes of LM of seeds exposed distinctive variation in seed size from 16.3 to 3.2 mm in length and 12.4 to 0.9 mm in width, shape varied from oval to triangular, and color from black to light brown. Oil content of nonedible seed ranged from 25 to 30% (w/w). Free fatty acid content of seed oil varied from 0.32 to 2.5 mg KOH/g. Moreover, ultra structural study of seeds via SEM showed variation in surface sculpturing, cell arrangement, cell shape, periclinal wall shape, margins, protuberances, and anticlinal wall shape. Surface sculpturing varied from rugged, reticulate, varrucose, papillate, and striate. Periclinal wall arrangements confirmed variation from rough, wavy, raised, depressed, smooth, and elevated whereas, anticlinal walls pattern showed variation from profuse undulating, smooth, raised, grooved, deep, curved, and depressed. It was concluded that SEM could be a latent and advanced technique in unveiling hidden micromorphological characters of nonedible oil yielding seeds which delivers valuable information to researchers and indigenous people for precise and authentic identification and recognition.

Identification of novel, non‐edible oil seeds via scanning electron microscopy as potential feedstock for green synthesis of biodiesel

Rozina

Zafar

et al. 2021

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In the present era, environmental glitches associated with extensive emission of greenhouse gases (GHG) and energy crises caused by exhausting fossil fuel reservoirs has diverted researcher's interest toward alternative and renewable energy sources. Biodiesel is a renewable, biodegradable, and sustainable alternative to petro‐diesel. Biodiesel synthesized from non‐edible seed oils is preferred due its cost effectiveness and eco‐friendly nature. Hence, our present study focused on investigation and identification of micromorphological characters of six novel, non‐edible seed oil feedstock for biodiesel production via scanning electron microscopy (SEM). Results of light microscopy of seeds revealed distinct variation in seed size (15.8–1.8 mm in length and 9.4–1.1 in width), shape (round to Cuneiform), and color (from black to yellowish green). Non‐edible seed oil content fall in range of 28–38% (wt/wt). Free fatty acid (FFA) content varied from 0.56 to 2.06 mg KOH/g. Multivariate analysis was performed to investigate correlation between three significant variables of seed oil yielding feedstock such as potential for biodiesel synthesis, oil content, and FFA content via principal component analysis. Ultra morphological investigation of seeds surfaces via SEM exhibited distinctive variation in surface sculpturing, cell arrangement, cell shape, periclinal wall shape, margins, protuberances, and anticlinal wall shape. Seed surface sculpturing varied from reticulate, semitectate, wrinkled, rugose, papillate, perforate, and striate. Periclinal wall arrangements confirmed variation from glabrous, raised, depressed, elevated, smooth, pentagonal, entire, and ripple margins. Whereas, anticlinal walls pattern demonstrated variation from angular, smooth, wavy, deep, dentate, entire, irregular, puzzled, elongated, curved, and depressed. Finally, it was concluded from obtained results that SEM could be a possible useful tool in disclosing veiled micromorphological characters of non‐edible oil yielding seeds, which provides useful information to researchers for their correct, authentic identification, and classification in modern synthetic system.