Identification Of Novel Nuclear Proteins Required For Meiotic Silencing By Unpaired Dna In Neurospora Crassa

Abstract

Introduction Materials and Methods Strains, media, culture conditions, crosses, and general techniques Genetic modification of N. crassa Gene expression analysis Confocal microscopy Results N. crassa gene ncu01917 is sad-7 SAD-7 is required for sexual development SAD-7 mutant cultures are indistinguishable from wild type cultures under standard growth conditions Sad-7 expression patterns are most similar to sad-4 SAD-7 homologs are present in a wide range of ascomycete fungi GFP-SAD7 fusion proteins are found in the nucleus, perinuclear region, and cytoplasmic foci of meiotic cells Discussion Acknowledgments Literature Cited IV.