2007
DOI: 10.1101/gr.5755407
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Identification of novel peptide hormones in the human proteome by hidden Markov model screening

Abstract: Peptide hormones are small, processed, and secreted peptides that signal via membrane receptors and play critical roles in normal and pathological physiology. The search for novel peptide hormones has been hampered by their small size, low or restricted expression, and lack of sequence similarity. To overcome these difficulties, we developed a bioinformatics search tool based on the hidden Markov model formalism that uses several peptide hormone sequence features to estimate the likelihood that a protein conta… Show more

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Cited by 255 publications
(279 citation statements)
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“…pNPs have a signal peptide (SP) and enter the secretory apparatus where dedicated proteases cleave them at mono-or dibasic cleavage sites (12) and where the maturing peptides are often further modified (13). pNPs are ubiquitous in eumetazoans, and genomics and mass spectrometry revealed the full neuropeptide repertoire of several species (14)(15)(16)(17)(18)(19)(20). Given their wide occurrence in metazoans, and importance in neuronal regulation, a global pNP phylogeny would further our knowledge of nervous system evolution.…”
mentioning
confidence: 99%
“…pNPs have a signal peptide (SP) and enter the secretory apparatus where dedicated proteases cleave them at mono-or dibasic cleavage sites (12) and where the maturing peptides are often further modified (13). pNPs are ubiquitous in eumetazoans, and genomics and mass spectrometry revealed the full neuropeptide repertoire of several species (14)(15)(16)(17)(18)(19)(20). Given their wide occurrence in metazoans, and importance in neuronal regulation, a global pNP phylogeny would further our knowledge of nervous system evolution.…”
mentioning
confidence: 99%
“…ECRG4 protein contains one canonical dibasic prohormone cleavage site corresponding to residues Lys69 and Arg70. Cleavage at this site has been demonstrated in vitro following transfection of a pancreatic cell line with the c2orf40 gene in which the Flag antigen sequence was inserted between Arg70 and Gln71 (Mirabeau et al, 2007). Western blotting of supernatant from Flag augurin-transfected cells revealed two immunoreactive bands consistent with secretion of an Arg70 cleaved 10 kDa peptide (augurin 71-148) and a smaller 8 kDa peptide.…”
Section: Introductionmentioning
confidence: 99%
“…Western blotting of supernatant from Flag augurin-transfected cells revealed two immunoreactive bands consistent with secretion of an Arg70 cleaved 10 kDa peptide (augurin 71-148) and a smaller 8 kDa peptide. It is suggested the 8 kDa peptide results from a second, unknown, C-terminal non-canonical cleavage site (Mirabeau et al, 2007).…”
Section: Introductionmentioning
confidence: 99%
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