Identification of novel plasmid-mediated extended-spectrum β-lactamase CTX-M-57 in Salmonella enterica serovar Typhimurium

Hopkins, Katie L.; Threlfall, E. J.; Karisik, E.; Wardle, J.K.

doi:10.1016/j.ijantimicag.2007.08.017

Cited by 24 publications

(27 citation statements)

References 6 publications

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“…CTX-M-55 has recently also been detected in other countries [23][24][25]. Most interestingly, subsequent to the first discovery of a novel CTX-M-64 in a Shigella sonnei isolate from Japan [6], we detected CTX-M-64 in three E. coli isolates from pets.…”

Section: Discussionmentioning

confidence: 72%

High prevalence of blaCTX-M extended-spectrum β-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China

Sun

Zeng

Chen

et al. 2010

Clinical Microbiology and Infection

153

106

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As a cause of community-acquired infections, extended-spectrum β-lactamase (ESBL)-producing Escherichia coli constitute an emerging public-health concern. Few data on the molecular epidemiology of ESBL-producing E. coli isolates from pets are available in China. Detection and characterization of ESBL genes (bla(CTX-M), bla(SHV) and bla(TEM)) was conducted among 240 E. coli isolates recovered from healthy and sick pets in South China from 2007 to 2008. The clonal relatedness of ESBL-producing E. coli isolates was assessed by pulsed field gel electrophoresis. ESBL-encoding genes were identified in 97 (40.4%) of the 240 isolates and 96 (40.0%) of them harbored CTX-M. The most common CTX-M types were CTX-M-14 (n = 45) and CTX-M-55 (n = 24). The recently reported CTX-M-64 was identified in three isolates. Isolates producing CTX-M-27, -15, -65, -24, -3 and -9 were also identified. Ten isolates carried two or three CTX-M types, with the combination of CTX-M-14 and CTX-M-55 being the most frequent (n = 6). ISEcp1 was identified in the upstream region of 93 out of the 107 bla(CTX-M) genes (86.9%). The sequence of the spacer region (45 bp) between ISEcp1 and the start codon of all bla(CTX-M-55) genes (except four) was identical to that of bla(CTX-M-64). No major clonal relatedness was observed among these CTX-M producers. It is suggested that the horizontal transfer of bla(CTX-M) genes, mediated by mobile elements, contributes to their dissemination among E. coli isolates from pets. Our finding of high prevalence of ESBL in E. coli of companion animal origin illustrates the importance of molecular surveillance in tracking CTX-M-producing E. coli strains in pets.

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Section: Discussionmentioning

confidence: 72%

High prevalence of blaCTX-M extended-spectrum β-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China

Sun

Zeng

Chen

et al. 2010

Clinical Microbiology and Infection

153

106

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“…CTX-M ESBLs are a rapidly growing group, which contains ESBLs encoded by more than 80 identified CTX-M genes (http://www.lahey.org/Studies/other.asp). In the genus Salmonella, 14 different CTX-M ␤-lactamases have been reported in several serotypes and over wide geographic areas (3,5,20,21,(36)(37)(38). We report here the characterization of the novel CTX-M-53 ESBL in S. enterica serotypes Westhampton and Senftenberg in France.…”

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confidence: 93%

Novel Plasmid-Encoded Ceftazidime-Hydrolyzing CTX-M-53 Extended-Spectrum β-Lactamase from Salmonella enterica Serotypes Westhampton and Senftenberg

Doublet

Granier

Robin

et al. 2009

Antimicrob Agents Chemother

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We describe the characterization of a novel CTX-M ␤-lactamase from Salmonella enterica. Four S. enterica isolates (three of serotype Westhampton and one of serotype Senftenberg) resistant to extended-spectrum cephalosporins (cefotaxime and ceftazidime) were recovered in 2004 from living cockles in three supermarkets located in distant geographic areas in France, which got their supplies from the same fishery. The isolates were found to produce a novel extended-spectrum ␤-lactamase (ESBL) belonging to the CTX-M-1 phylogenetic group and named CTX-M-53. The CTX-M-53 ␤-lactamase harbored the substitution Asp240Gly, like the CTX-M-15 enzyme, which is specifically implicated in a higher catalytic efficiency against ceftazidime. The bla CTX-M-53 gene was located on a mobilizable 11-kb plasmid, pWES-1. The complete sequence of pWES-1 revealed the presence of a novel insertion sequence, ISSen2, and an IS26 element upstream and downstream of the bla CTX-M-53 gene, respectively; however, transposition assays of the bla CTX-M-53 gene were unsuccessful. IS26 elements may have contributed to the acquisition of the bla CTX-M-53 gene. Interestingly, the mobilization module of the pWES-1 plasmid was similar to that of quinolone resistance plasmids (carrying the qnrS2 gene) from aquatic sources. Although belonging to two serotypes differentiated on the basis of the O-antigen structure (E1 or E4 groups), the isolates were found to be genetically indistinguishable by pulsed-field gel electrophoresis. Multilocus sequence typing showed that the isolates of serotype Westhampton had a sequence type, ST14, common among isolates of serotype Senftenberg. This is the first characterization of the CTX-M-53 ESBL, which represents an additional ceftazidime-hydrolyzing CTX-M enzyme.Salmonella enterica is a frequent pathogen of animals and humans. Food-borne diseases caused by this species represent an important public health problem worldwide. Extendedspectrum cephalosporins (ESC) and fluoroquinolones are often used in the treatment of invasive cases of salmonellosis. However, the emergence of extended-spectrum ␤-lactamases (ESBLs) in Enterobacteriaceae is an increasing problem worldwide, compromising the utilization of these drugs in the treatment of complicated Salmonella infections. Moreover, there is an increasing number of reports of ESBL-producing S. enterica strains throughout the world (3, 36). These strains, isolated mostly in hospitalized patients, produced plasmid-mediated class A ESBLs belonging to the TEM, SHV, cefotaximase (CTX-M), or PER families (3).CTX-M ESBLs are class A ESBLs that in general possess a higher level of hydrolytic activity against cefotaxime (and ceftriaxone) than against ceftazidime but that are inhibited by clavulanic acid, sulbactam, and tazobactam (5, 9, 26). On the basis of their amino acid sequences, the CTX-M enzymes have been classified into five major phylogenetic branches, namely the CTX-M-1, -2, -8, -9, and -25 groups (5, 9, 26; http://www.lahey.org /Studies/other.asp). CTX-M ESBLs are a rapidly grow...

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“…with diminished susceptibility to ceftriaxone, obtained in 2003-2006, 9 were found to have ESBL genes and 6 had plasmid AmpC genes; the ESBL genes detected were bla SHV-5 , bla CTX-M group 1 , and bla CTX-M group 9 .…”

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confidence: 99%

“…(1). Ceftriaxone resistance among Salmonella spp., however, has been reported worldwide (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13), and common resistance mechanisms include the production of plasmid-mediated AmpC b-lactamases and extended-spectrum b-lactamases (ESBLs) (2)(3)(4)(5)(7)(8)(9). A previous study involving 78 clinical isolates of Salmonella spp.…”

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confidence: 99%

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Ceftriaxone Resistance and Genes Encoding Extended-Spectrum ^|^beta;-Lactamase among Non-Typhoidal Salmonella Species from a Tertiary Care Hospital in Kuala Lumpur, Malaysia

et al. 2012

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Identification of novel plasmid-mediated extended-spectrum β-lactamase CTX-M-57 in Salmonella enterica serovar Typhimurium

Cited by 24 publications

References 6 publications

High prevalence of blaCTX-M extended-spectrum β-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China

High prevalence of blaCTX-M extended-spectrum β-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China

Novel Plasmid-Encoded Ceftazidime-Hydrolyzing CTX-M-53 Extended-Spectrum β-Lactamase from Salmonella enterica Serotypes Westhampton and Senftenberg

Ceftriaxone Resistance and Genes Encoding Extended-Spectrum ^|^beta;-Lactamase among Non-Typhoidal Salmonella Species from a Tertiary Care Hospital in Kuala Lumpur, Malaysia

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