Identification of novel prognostic indicators for oral squamous cell carcinoma based on proteomics and metabolomics

Yao, Zhitao; An, Wei; Tuerdi, Maimaitituxun; Zhao, Jin

doi:10.1016/j.tranon.2023.101672

Cited by 6 publications

(1 citation statement)

References 43 publications

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“…The preparation of saliva samples for metabolomics analysis is quite simple. In general, the procedures involve protein precipitation with acetonitrile [ 189 , 190 , 191 , 192 , 193 , 194 , 195 ], methanol [ 196 , 197 , 198 ], or a combination of methanol/acetonitrile (1:1, v / v ) [ 199 , 200 , 201 ], methanol/water [ 202 , 203 , 204 , 205 , 206 ], and methanol/acetonitrile/water (2:2:1, v / v / v ) [ 207 ]. Other studies have used isopropanol [ 208 ] or acetone [ 209 ], which requires evaporation and resuspension in an appropriate solvent before chromatographic analysis.…”

Section: Underexplored Specimens In Clinical Metabolomicsmentioning

confidence: 99%

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

de Souza,

Pereira,

de Sá

et al. 2024

Metabolites

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This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, and cerebrospinal fluid) under the perspective of clinical metabolomics. These samples are interesting for metabolomics studies as they reflect the status of living organisms and can be applied for diagnostic purposes and biomarker discovery. Pre-collection and collection procedures are critical, requiring protocols to be standardized to avoid contamination and bias. Such procedures must consider cleaning the collection area, sample stimulation, diet, and food and drug intake, among other factors that impact the lack of homogeneity of the sample group. Precipitation of proteins and removal of salts and cell debris are the most used sample preparation procedures. This review intends to provide a global view of the practical aspects that most impact results, serving as a starting point for the designing of metabolomic experiments.

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Section: Underexplored Specimens In Clinical Metabolomicsmentioning

confidence: 99%

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

de Souza,

Pereira,

de Sá

et al. 2024

Metabolites

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Energy Metabolic Profile in Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma: A Preliminary Landscape of Warburg Effect in Oral Cancer

Gonçalves,

da Silva Bittencourt,

Barbosa

et al. 2024

Molecular Carcinogenesis

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We hypothesized that cell energy metabolic profiles correlate with normal, dysplastic, and tumor cell/tissue statuses and may be indicators of aggressiveness in oral squamous cell carcinoma (OSCC) cells. The energy‐related proteins that were differentially expressed in human OSCC fragments (n = 3) and their adjacent epithelial tissue (TAE) were verified using mass spectrometry (MS). Immunohistochemistry for 4‐hydroxynonenal (4‐HNE) was performed to evaluate the oxidative stress patterns in OSCC (n = 10), epithelial dysplasia (n = 9), and normal epithelial (n = 4) biopsies. The metabolic energy profile of OSCC aggressiveness was investigated in human OSCC cell lines with different levels of epithelial–mesenchymal transition proteins. The genes associated with the proteins found by MS in this study were analyzed using survival analysis (OS), whereas the genes associated with a poorer prognosis were analyzed using context‐specific expression, Gene Ontology (GO) and Cancer Hallmarks for function enrichment analysis. The rationale for all experimental approach was to investigate whether the variation in energy metabolism profile accompanies the different phenotypes (from epithelial to mesenchymal) during the epithelial‐mesenchymal transition. All OSCC fragments exhibited an increase in glycolysis‐related proteins and a decrease in mitochondrial activity compared to the TAE region (p < 0.05), probably due to the downregulation of pyruvate dehydrogenase and antioxidant proteins. Additionally, the OSCC cell lines with a mesenchymal profile (SCC4, SCC9, and SCC25) had a lower mitochondrial mass and membrane potential and generated lower levels of reactive oxygen and nitrogen species than the TAE region. When we analyzed 4‐HNE, the reactive species levels were increased in the epithelial regions of OSCC and potentially malignant lesions. A decrease in the levels of 4‐HNE/reactive species was observed in the connective tissue underlying the dysplastic regions and the OSCC invasion zone. Based on this scenario, aggressive OSCC is associated with high glycolytic and oxidative metabolism and low mitochondrial and antioxidant activities, which vary according to the differentiation level of the tumor cells and the stage of carcinogenesis.

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Metabolomic insights into idiopathic xerostomia: The central role of caffeine metabolism in salivary biochemistry

Hou,

Cui,

et al. 2025

Archives of Oral Biology

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Identification of novel prognostic indicators for oral squamous cell carcinoma based on proteomics and metabolomics

Cited by 6 publications

References 43 publications

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

Energy Metabolic Profile in Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma: A Preliminary Landscape of Warburg Effect in Oral Cancer

Metabolomic insights into idiopathic xerostomia: The central role of caffeine metabolism in salivary biochemistry

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