Identification of novel QTL contributing to barley yellow mosaic resistance in wild barley (Hordeum vulgare spp. spontaneum)

Pan, Yuhan; Jun, Zhu; Hong, Yi; Zhang, Mengna; Lv, Chao; Guo, Bin; Shen, Huiquan; Xu, Xiao; Xu, Ran

doi:10.1186/s12870-021-03321-x

Cited by 7 publications

(4 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rph15 is a gene derived from wild barley conferring resistance to leaf rust (Chen et al 2021). A novel QTL qRYM-2Ha contributing to barley yellow mosaic resistance was identified in wild barley (Pan et al 2021). Association and expression analysis revealed that Tibetan wild barley offers elite alleles of HvHKT1 and HvHKT2 conferring salinity tolerance (Qiu et al 2011).…”

Section: Discussionmentioning

confidence: 99%

HvNCX, a prime candidate gene for the novel qualitative locus qS7.1 associated with salinity tolerance in barley

et al. 2023

Self Cite

View full text Add to dashboard Cite

Key Message A major QTL (qS7.1) for salinity damage score and Na+ exclusion was identified on chromosome 7H from a barley population derived from a cross between a cultivated variety and a wild accession. qS7.1 was fine-mapped to a 2.46 Mb physical interval and HvNCX encoding a sodium/calcium exchanger is most likely the candidate gene. Abstract Soil salinity is one of the major abiotic stresses affecting crop yield. Developing salinity-tolerant varieties is critical for minimizing economic penalties caused by salinity and providing solutions for global food security. Many genes/QTL for salt tolerance have been reported in barley, but only a few of them have been cloned. In this study, a total of 163 doubled haploid lines from a cross between a cultivated barley variety Franklin and a wild barley accession TAM407227 were used to map QTL for salinity tolerance. Four significant QTL were identified for salinity damage scores. One (qS2.1) was located on 2H, determining 7.5% of the phenotypic variation. Two (qS5.1 and qS5.2) were located on 5H, determining 5.3–11.7% of the phenotypic variation. The most significant QTL was found on 7H, explaining 27.8% of the phenotypic variation. Two QTL for Na+ content in leaves under salinity stress were detected on chromosomes 1H (qNa1.1) and 7H(qNa7.1). qS7.1 was fine-mapped to a 2.46 Mb physical interval using F4 recombinant inbred lines. This region contains 23 high-confidence genes, with HvNCX which encodes a sodium/calcium exchanger being most likely the candidate gene. HvNCX was highly induced by salinity stress and showed a greater expression level in the sensitive parent. Multiple nucleotide substitutions and deletions/insertions in the promoter sequence of HvNCX were found between the two parents. cDNA sequencing of the HvNCX revealed that the difference between the two parents is conferred by a single Ala77/Pro77 amino acid substitution, which is located on the transmembrane domain. These findings open new prospects for improving salinity tolerance in barley by targeting a previously unexplored trait.

show abstract

Section: Discussionmentioning

confidence: 99%

HvNCX, a prime candidate gene for the novel qualitative locus qS7.1 associated with salinity tolerance in barley

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rph15 is a gene derived from wild barley conferring resistance to leaf rust (Chen et al 2021). A novel QTL qRYM-2Ha contributing to barley yellow mosaic resistance was identi ed in wild barley (Pan et al 2021). Association and expression analysis revealed that Tibetan wild barley offers elite alleles of HvHKT1 and HvHKT2 conferring salinity tolerance (Qiu et al 2011).…”

Section: Discussionmentioning

confidence: 99%

HvNCX, a prime candidate gene for the novel qualitative locus qS7.1 controlling salinity tolerance and Na+ exclusion in wild barley

Jun

Zhou

Fan

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Soil salinity is one of the major abiotic stresses affecting crop yield. Developing salinity-tolerant varieties is critical for minimizing economic penalties caused by salinity and providing solutions for global food security. Many genes/QTL for salt tolerance have been reported in barley but only a few of them have been map-cloned. In this study, a total of 163 doubled haploid lines from a cross between a cultivated barley variety Franklin and the wild barley accession Tam407227 were used to map QTL for salinity tolerance. Two major QTL for salinity tolerance were identified on chromosomes 5H (qS5.1) and 7H (qS7.1), accounting for 11.7% and 27.8% of the phenotypic variation, respectively. Two QTL for Na+ content in leaves under salinity stress were detected on chromosomes 1H (qNa1.1) and 7H(qNa7.1). qS7.1 was fine-mapped to a 2.46 Mb physical interval using F4 recombinant inbred lines (RILs). This region contains 23 high-confidence genes, with HvNCX which encodes a sodium/calcium exchanger being most likely the candidate gene. The HvNCX was highly induced by salinity stress and showed a greater expression level in a sensitive variety. cDNA sequencing of the HvNCX revealed that the difference between the two parents is conferred by a single Ala77Pro amino acid substitution, which is located on the transmembrane domain. These findings open new prospects for improving salinity tolerance in barley by targeting a previously unexplored trait.

show abstract

“…Severity scores of 0 to 4 represent the damage caused by virus infection from light to severe ( Fig. 1 ): 0 means healthy leaves with no symptoms of disease, 1 represents typical chlorotic spots, 2 indicates a quarter of the leaves display a yellow mosaic pattern, 3 represents more than half of the leaves display a yellow mosaic pattern, and 4 indicates more than three-quarters of the leaves display yellow mosaic pattern ( Pan et al, 2021 ). Afterwards, a standardized area under the disease progress steps (sAUDPS) index was used for the evaluation of disease resistance, which can effectively combine and transform multiple investigations of disease grades into a single value for further GWAS analysis ( Simko & Piepho, 2012 ).…”

Section: Methodsmentioning

confidence: 99%

Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease

Pan

Jun

Hong

et al. 2022

PeerJ

Self Cite

View full text Add to dashboard Cite

Background The disease caused by Barley yellow mosaic virus (BaYMV) infection is a serious threat to autumn-sown barley (Hordeum vulgare L.) production in Europe, East Asia and Iran. Due to the rapid diversification of BaYMV strains, it is urgent to discover novel germplasm and genes to assist breeding new varieties with resistance to different BaYMV strains, thus minimizing the effect of BaYMV disease on barley cropping. Methods A natural population consisting of 181 barley accessions with different levels of resistance to BaYMV disease was selected for field resistance identification in two separate locations (Yangzhou and Yancheng, Jiangsu Province, China). Additive main effects and multiplicative interaction (AMMI) analysis was used to identify accessions with stable resistance. Genome-wide association study (GWAS) of BaYMV disease resistance was broadly performed by combining both single nucleotide polymorphisms (SNPs) and specific molecular markers associated with the reported BaYMV disease resistance genes. Furthermore, the viral protein genome linked (VPg) sequences of the virus were amplified and analyzed to assess the differences between the BaYMV strains sourced from the different experimental sites. Results Seven barley accessions with lower standardized Area Under the Disease Progress Steps (sAUDPS) index in every environment were identified and shown to have stable resistance to BaYMV disease in each assessed location. Apart from the reported BaYMV disease resistance genes rym4 and rym5, one novel resistance locus explaining 24.21% of the phenotypic variation was identified at the Yangzhou testing site, while two other novel resistance loci that contributed 19.23% and 19.79% of the phenotypic variation were identified at the Yancheng testing site, respectively. Further analysis regarding the difference in the VPg sequence of the predominant strain of BaYMV collected from these two testing sites may explain the difference of resistance loci differentially identified under geographically distinct regions. Our research provides novel genetic resources and resistance loci for breeding barley varieties for BaMYV disease resistance.

show abstract

Identification of novel QTL contributing to barley yellow mosaic resistance in wild barley (Hordeum vulgare spp. spontaneum)

Cited by 7 publications

References 69 publications

HvNCX, a prime candidate gene for the novel qualitative locus qS7.1 associated with salinity tolerance in barley

HvNCX, a prime candidate gene for the novel qualitative locus qS7.1 associated with salinity tolerance in barley

HvNCX, a prime candidate gene for the novel qualitative locus qS7.1 controlling salinity tolerance and Na+ exclusion in wild barley

Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease

Contact Info

Product

Resources

About