Identification of novel QTL for resistance to Fusarium head blight in a tetraploid wheat population

Ruan, Yuefeng; Comeau, A.; Langevin, François; Hucl, P.; Clarke, J. M.; Brûlé‐Babel, Anita L.; Pozniak, Curtis

doi:10.1139/gen-2012-0110

Cited by 44 publications

(39 citation statements)

References 53 publications

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“…causing FHB (Mesterházy et al 2005). Resistance to FHB is a complex trait, with polygenic inheritance and its expression is influenced by the environment (Liu et al 2009;Ruan et al 2012;Buerstmayr and Buerstmayr 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Khaledi¹,

Taheri²,

Falahati-Rastegar³

2018

Journal of Plant Protection Research

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Fusarium graminearum and F. culmorum are the causal agents of Fusarium head blight (FHB) in cereal crops worldwide. Application of resistant cultivars is the most effective and economic method for management of FHB and reducing mycotoxin production in wheat. Understanding the physiological and biochemical mechanisms involved in basal resistance of wheat to FHB disease is limited. In this research, after screening resistance levels of eighteen wheat cultivars planted in Iran, Gaskozhen and Falat were identified as partially resistant and susceptible wheat cultivars against Fusarium spp., respectively. Also, we investigated the role of hydroxyl radical (OH − ), nitric oxide (NO), callose deposition, lipid peroxidation and protein content in basal resistance of wheat to the hemi-biotrophic and necrotrophic Fusarium species causing FHB. Nitric oxide as a signaling molecule may be involved in physiological and defensive processes in plants. Our results showed that NO generation increased in seedlings and spikes of wheat cultivars after inoculation with Fusarium species. We observed earlier and stronger callose deposition at early time points after infection by Fusarium spp. isolates than in non-infected plants, which was positively related to the resistance levels in wheat cultivars. Higher levels of OH − and malondialdehyde (MDA) accumulation (as a marker of lipid peroxidation) were observed in the Falat than in the Gaskozhen cultivar, under non-infected and infected conditions. So, estimation of lipid peroxidation could be useful to evaluate cultivars' susceptibility. These findings can provide novel insights for better recognition of physiological and biochemical markers of FHB resistance, which could be used for rapid screening of resistance levels in wheat cultivars against this destructive fungal disease.

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Section: Introductionmentioning

confidence: 99%

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Khaledi¹,

Taheri²,

Falahati-Rastegar³

2018

Journal of Plant Protection Research

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“…Tetraploid relatives have proved to be an important source of FHB resistance (Prat et al 2014). QTLs conferring resistance were identified in T. carthlicum (Somers et al 2006), T. dicoccum (Buerstmayr et al 2012;Ruan et al 2012;Zhang et al 2014) and T. dicoccoides (Otto et al 2002;Chen et al 2007;Kumar et al 2007;Buerstmayr et al 2013), in some cases were mapped in similar positions as resistance QTLs described for hexaploid wheat.…”

Section: Cereal Research Communications 45 2017mentioning

confidence: 91%

A valuable QTL for Fusarium head blight resistance from Triticum turgidum L. ssp. dicoccoides has a stable expression in durum wheat cultivars

Soresi

Zappacosta

Garayalde

et al. 2017

Cereal Research Communications

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Langdon(Dic-3A)-10 line carrying the QTL Qfhs.ndsu-3AS from T. turgidum ssp. dicoccoides that confers Type II resistance to Fusarium head blight (FHB) was crossed with Argentinean durum wheat cultivars. F4 progeny were screened with the microsatellite locus Xgwm2, tightly linked to the Qfhs.ndsu-3A region. Reaction of these plants and parents to FHB was evaluated at 7, 14 and 21 days post-inoculation (dpi) with F. graminearum; severity (% symptomatic spikelets/spike) and AUDPC (area under disease progress curve) were calculated. F4 progeny carrying the resistance allele in heterozygous or in homozygous condition showed significantly lower scab damage at 21 dpi and slower progress of disease than cultivated parents. Our results indicate that the resistance Qfhs.ndsu-3AS has a stable dominance expression in genetic backgrounds of durum cultivars and demonstrate that the linked microsatellite is an effective molecular tool for resistance screening. This work offers valuable information for Qfhs.ndsu-3AS utilization in wheat breeding programs.

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“…These markers are commonly used for mapping, to evaluate genetic diversity, and for association mapping studies (Marone et al 2012). TDF#30 (Group B2) was aligned with the Wpt-8125 marker (Identity = 98%, e-value = 2 × 10 −83 ), mapped to chromosome 5BL at 2.5 cM from the QFhb.usw-5B3 QTL that is associated with FHB resistance (Ruan et al 2012).…”

Section: Homology Searches Against Molecular Markers Linked To Resistmentioning

confidence: 99%

Identification of genes induced by Fusarium graminearum inoculation in the resistant durum wheat line Langdon(Dic-3A)10 and the susceptible parental line Langdon

Soresi

Carrera

Echenique

et al. 2015

Microbiological Research

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The wheat recombinant chromosome inbred line LDN(Dic-3A)10, obtained through introgression of a Triticum dicoccoides disomic chromosome 3A fragment into Triticum turgidum spp. durum var. Langdon, is resistant to fusarium head blight (FHB) caused by Fusarium graminearum. To identify genes involved in FHB resistance, we used a cDNA-AFLP approach to compare gene expression between LDN(Dic-3A)10 and the susceptible parental line LDN at different time points post-inoculation. In total, 85 out of the ∼ 500 transcript-derived fragments (TDFs) were found to be differentially expressed: 36 and 19% were upregulated in LDN(Dic-3A)10 and LDN, respectively, whereas 45% were induced in both genotypes. Several of the cloned TDFs showed similarity to proteins involved in specific recognition of plant pathogens or associated with early responses to infection. Some TDFs specific to the inoculation response did not show similarity to characterized proteins. The availability of T. aestivum genome sequences allowed the in silico mapping of 28 TDFs and the acquirement of the corresponding gene sequences and, in some cases, their regulatory regions. Analysis of promoter regions revealed the potential existence of shared transcription regulation mechanisms. For instance, three TDF-associated genes contained binding sites for WRKY transcription factors, which have been implicated in the regulation of genes associated with pathogen defense, and three for abscisic acid-responsive element (ABRE). Collectively, our results revealed specific pathogen recognition in the interactions of LDN and LDN(Dic-3A)10 with F. graminearum. Such recognition leads to changes in the expression of several transcripts, attributable to the presence of the wheat QTL Qfhs.ndsu-3AS.

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Identification of novel QTL for resistance to Fusarium head blight in a tetraploid wheat population

Cited by 44 publications

References 53 publications

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

A valuable QTL for Fusarium head blight resistance from Triticum turgidum L. ssp. dicoccoides has a stable expression in durum wheat cultivars

Identification of genes induced by Fusarium graminearum inoculation in the resistant durum wheat line Langdon(Dic-3A)10 and the susceptible parental line Langdon

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