Identification of Novel QTLs for Isolate-Specific Partial Resistance to Plasmodiophora brassicae in Brassica rapa

Chen, Jingjing; Jing, Jing; Zhan, Zhongxiang; Zhang, Teng; Zhang, Chunyu; Piao, Zhongyun

doi:10.1371/journal.pone.0085307

Cited by 89 publications

(97 citation statements)

References 53 publications

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“…napus through homeologous recombination between A, C and R genomes is eminently possible. Several cytological studies have shown high frequency of homologous chromosome pairing among C subgenomes of Brassica species [14, 30, 38]. Our results showed that the C subgenomes in Brassicoraphanus (RRCC, 2n = 36) and B .…”

Section: Discussionsupporting

confidence: 57%

Cytological and morphological analysis of hybrids between Brassicoraphanus, and Brassica napus for introgression of clubroot resistant trait into Brassica napus L

et al. 2017

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Interspecific hybridization is a powerful tool for improvement of crop species, it has the potential to broaden the genetic base and create new plant forms for breeding programs. Synthetic allopolyploid is a widely-used model for the study of genetic recombination and fixed heterosis in Brassica. In Brassica napus breeding, identification and introgression of new sources of clubroot resistance trait from wild or related species into it by hybridization is a long-term crop management strategy for clubroot disease. Radish (Raphanus sativus L.) is a close relative of the Brassica and most radish accessions are immune to the clubroot disease. A synthesized allotetraploid Brassicoraphanus (RRCC, 2n = 36) between R. sativus cv. HQ-04 (2n = 18, RR) and Brassica oleracea var. alboglabra (L.H Bailey) (2n = 18, CC) proved resistant of multiple clubroot disease pathogen P. brassicae. To predict the possibility to transfer the clubroot resistance trait from the RR subgenome of allotetraploid Brassicoraphanus (RRCC, 2n = 36) into Brassica napus (AACC, 2n = 38), we analyzed the frequency of chromosome pairings in the F1 hybrids produced from a cross between B. napus cv. HS5 and the allotetraploid, characterize the genomic composition of some backcrossed progeny (BC1) using GISH, BAC-FISH and AFLP techniques. The level of intergenomic pairing between A and R genomes in the F1 hybrid was high, allosyndetic bivalents formed in 73.53% PMCs indicative of significant level of homeologous recombination between two genomes and high probability of incorporating chromosomal segments/genes from R-genome into A/C-genomes. The BC1 plants inherited variant extra R chromosomes or fragments from allotetraploid as revealed by GISH and AFLP analysis. 13.51% BC2 individuals were resistant to clubroot disease, and several resistance lines had high pollen fertility, Overall, the genetic material presented in this work represents a potential new genetic resource for practical use in breeding B. napus clubroot resistant cultivars.

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Section: Discussionsupporting

confidence: 57%

Cytological and morphological analysis of hybrids between Brassicoraphanus, and Brassica napus for introgression of clubroot resistant trait into Brassica napus L

et al. 2017

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“…(), Chen et al . () and Zhang et al . () to be linked with Crr1 gene, were not associated with resistance to any of the pathotypes.…”

Section: Resultsmentioning

confidence: 97%

“…Similarly, the resistance gene in the DH population used in this study (derived from ECD 04), when screened against the old Canadian P. brassicae pathotypes 2, 3, 5, 6 and 8, was traced to the same genomic region where CRa and/or CRb Kato are found (Zhang et al, 2015a). In contrast, the resistance gene in ECD 04-derived populations mapped to the A08 chromosome when screened against Chinese P. brassicae pathotypes (mainly pathotype 4); this is a region where the Crr1 gene has been reported (Chen et al, 2013;Zhang et al, 2015b). Similarly, two major QTLs on the A03 and A08 chromosomes were found to confer resistance to multiple German (four), Swedish (two) and French (one) P. brassicae field and single-spore isolates in an ECD 04-derived DH population (Werner et al, 2008).…”

Section: Discussionmentioning

confidence: 98%

“…Similarly, an ECD 04 (AA) 9 kale (CC) cross was used by the breeder Norddeutsche Pflanzenzucht (NPZ)-Lembke to develop the winter B. napus 'Mendel' in 2000, which also showed a high level of resistance to P. brassicae populations from Germany and other European countries (Diederichsen & Sacristan, 1996;Diederichsen et al, 2006). ECD 04 also showed a high level of resistance to Chinese P. brassicae populations, which are classified predominantly as pathotype 4 on the differential system of Williams (1966) (Chen et al, 2013). The current study showed that many clubroot-resistant commercial canola cultivars in Canada derive their resistance from ECD 04 or Mendel, and these conferred resistance to the old P. brassicae pathotypes 2, 3, 5, 6 and 8.…”

Section: Discussionmentioning

confidence: 99%

“…The markers from the A08 chromosome comprised three markers (cnu_m090a, sau_um353a and A08-300) reported by Chen et al (2013) and Zhang et al (2015b) to be tightly linked to clubroot resistance derived from ECD 04, 20 markers from the 1.6 cM genomic region between the SSR markers BRMS-173 and BRMS-088 reported by Suwabe et al (2012) to be tightly linked to the Crr1 gene, and 14 markers flanking BRMS-173 and BRMS-088.…”

Section: Pcr and Molecular Marker Genotypingmentioning

confidence: 99%

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Potential loss of clubroot resistance genes from donor parent Brassica rapa subsp. rapifera (ECD 04) during doubled haploid production

et al. 2018

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Clubroot resistance derived from the oilseed rape/canola Brassica napus ‘Mendel’ has been overcome in some fields in Alberta, Canada, by the emergence of ‘new’ strains of the protist Plasmodiophora brassicae. Resistance to the pathogen was assessed in 112 doubled haploid (DH) lines, derived from B. rapa subsp. rapifera (European clubroot differential (ECD) 04). The lines were evaluated against five single‐spore isolates representing the ‘old’ pathotypes 2, 3, 5, 6 and 8, and 15 field populations representing new strains of P. brassicae. The disease severity index (ID%) data revealed that none of the DH lines were resistant or moderately resistant to the new pathotype 5X (field populations L‐G1, L‐G2, L‐G3) and D‐G3, while 3–42% were resistant or moderately resistant to the other 11 new strains. Using the mean ID induced by the old pathotype 3 (approx. 13.5%) as the baseline, clubroot severity increased by 300–600% when inoculated with the new pathotypes. A significant finding of this study was the fact that ECD 04 showed absolute resistance to all of the old and new P. brassicae strains while the B. napus ‘Mendel’, although resistant to all of the old pathotypes, was resistant to only about 50% of the new strains. Similarly, all of the selected clubroot‐resistant commercial canola cultivars evaluated in this study were susceptible to 87% of the new P. brassicae strains. The molecular data revealed that the breakdown of clubroot resistance in Mendel and the canola cultivars was in part due to the non‐inheritance of the Crr1 gene on the A08 chromosome from ECD 04.

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Identification of a genomic region containing genes involved in resistance to four pathotypes of Plasmodiophora brassicae in Brassica rapa turnip ECD02

Rahaman

Strelkov

et al. 2022

The Plant Genome

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Clubroot, caused by Plasmodiophora brassicae, is an important disease of brassica crops worldwide. Vegetable turnip (Brassica rapa L.) have proven to be a source of clubroot resistance genes effective against many pathotypes of P. brassicae. The F 1 progeny from the cross B. rapa canola ACDC (susceptible, S) × B. rapa turnip ECD02 (resistant, R) were backcrossed with ACDC, then self-pollinated to produce BC 1 S 1 lines. All the F 1 plants were resistant to four pathotypes (3A, 3D, 3H, and 5X) of P. brassicae. Segregation for R and S in BC 1 to each pathotype was 1:1 and resistance reactions were highly correlated. From whole genome sequencing, 192.1 M sequences with 96% template coverage from ECD02, and 478.9 M sequences with 92% coverage from ACDC, were aligned with the reference genome of B. rapa.Genotyping-by-sequencing was performed on the BC 1 population. The number of aligned short reads per plant in the BC 1 ranged from 1.4 to 8.5 M sequences with 4-8% template coverage. We obtained 1,344 high-quality single-nucleotide polymorphism (SNP) loci with a mean missing rate at 0.27% and distributed them on 10 chromosomes. A single co-localized quantitative trait loci (QTL), designated as Rcr9 ECD02 on chromosome A08, conferred resistance to the four pathotypes. The QTL explained 68.9-74.4% of phenotypic variation with the logarithm of the odds values of 24.3 to 31.1. Bulked segregant analysis was performed, and 14 SNP markers linked to the gene were developed using the Kompetitive Allele Specific PCR.Rcr9 ECD02 was mapped into an interval of 2.2 cM, flanked by CF_A08_10664692 and CF_A08_12230973, which spanned 1.51 Mb on the chromosome and

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Identification of Novel QTLs for Isolate-Specific Partial Resistance to Plasmodiophora brassicae in Brassica rapa

Cited by 89 publications

References 53 publications

Cytological and morphological analysis of hybrids between Brassicoraphanus, and Brassica napus for introgression of clubroot resistant trait into Brassica napus L

Cytological and morphological analysis of hybrids between Brassicoraphanus, and Brassica napus for introgression of clubroot resistant trait into Brassica napus L

Potential loss of clubroot resistance genes from donor parent Brassica rapa subsp. rapifera (ECD 04) during doubled haploid production

Identification of a genomic region containing genes involved in resistance to four pathotypes of Plasmodiophora brassicae in Brassica rapa turnip ECD02

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