Identification of novel sources of bacterial leaf blight resistance in wild species of rice

Abstract

Wild species of rice possess tremendous genetic variations and harbour resistance genes for biotic stresses. Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is a major disease affecting rice production globally. The current study characterized 116 accessions from 17 species of Oryza for BB disease during three seasons viz., kharif 2020, rabi 2020–21, kharif 2021 using an isolate of Xanthomonas oryzae pv. oryzae (Xoo) strain IX-020. A total of 40 accessions including Oryza rufipogon, O. nivara, O. officinalis and O. australiensis showed consistence resistance to the bacterial blight disease across the seasons. These accessions were further subjected to molecular characterization using 11 Xa genes viz., Xa4, xa5, xa13, Xa21, Xa23, Xa27(t), Xa32(t), Xa33, Xa35(t), Xa38 and xa41 with gene-specific markers to ascertain the novelty. Some key resistance genes such as Xa4, Xa23, Xa27(t), Xa32(t), Xa33, Xa35(t) and xa41 were detected in multiple accessions, with O. rufipogon and O. eichingeri harbouring particularly complex combinations of these genes. Notably, several accessions viz., IC521672 (O. nivara), EC861665 (O. officinalis), EC861677 (O. latifolia), EC861711 (O. punctata) and EC861738 (O. eichingeri) did not show the presence of any known genes indicating the possibility of novel genetic loci conferring BB resistance in these wild species. These promising accessions identified in the study are potential novel sources for bacterial leaf blight resistance in rice and will be useful for the development of durable bacterial blight resistance rice cultivars.