Identification of novel sRNAs involved in biofilm formation, motility and fimbriae formation in Escherichia coli

Bak, Geunu; Lee, Jungmin; Suk, Shinae; Kim, Daun; Lee, Ji Young; Kim, Kwang-sun; Choi, Byong‐Seok; Lee, Younghoon

doi:10.1038/srep15287

Cited by 91 publications

(92 citation statements)

References 72 publications

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“…In both LB and YESCA, the deletion of hfq had the greatest effect, essentially abolishing biofilm formation (Fig. C and D), consistent with a previous study carried out in LB (Bak et al ., ). Most of the other deletions had only modest effects, although deletion of mcaS reduced biofilm almost two‐fold in LB, and deletion of arcZ , dsrA or gadY all reduced biofilm at least two‐fold in YESCA.…”

Section: Resultsmentioning

confidence: 97%

Alternative pathways for Escherichia coli biofilm formation revealed by sRNA overproduction

Parker

Cureoglu

Lay

et al. 2017

Molecular Microbiology

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Small regulatory RNAs have major roles in many regulatory circuits in Escherichia coli and other bacteria, including the transition from planktonic to biofilm growth. We tested Hfq-dependent sRNAs in E. coli for their ability, when overproduced, to inhibit or stimulate biofilm formation, in two different growth media. We identify two mutually exclusive pathways for biofilm formation. In LB, PgaA, encoding an adhesion export protein, played a critical role; biofilm was independent of the general stress factor RpoS or CsgD, regulator of curli and other biofilm genes. The PgaA-dependent pathway was stimulated upon overproduction of DsrA, via negative regulation of H-NS, or of GadY, likely by titration of CsrA. In YESCA (Yeast Extract Casamino acids) media, biofilm was dependent upon RpoS and CsgD, but independent of PgaA; RpoS appears to indirectly negatively regulate the PgaA-dependent pathway in YESCA medium. Deletions of most sRNAs had very little effect on biofilm, although deletion of hfq, encoding an RNA chaperone, was defective in both LB and YESCA. Deletion of ArcZ, a small RNA activator of RpoS, decreased biofilm in YESCA; only a portion of this defect could be bypassed by overproduction of RpoS. Overall, sRNAs highlight different pathways to biofilm formation.

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Section: Resultsmentioning

confidence: 97%

Alternative pathways for Escherichia coli biofilm formation revealed by sRNA overproduction

Parker

Cureoglu

Lay

et al. 2017

Molecular Microbiology

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“…Fimbrial expression can be regulated post-transcriptionally. E. coli small RNA (sRNA) have been shown to regulate the transcription of some fimbriae (265, 266). However, it is not known whether sRNA also regulate ETEC fimbriae.…”

Section: Regulation Of Fimbrial Expressionmentioning

confidence: 99%

Animal EnterotoxigenicEscherichia coli

2016

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Enterotoxigenic Escherichia coli (ETEC) is the most common cause of E. coli diarrhea in farm animals. ETEC are characterized by the ability to produce two types of virulence factors; adhesins that promote binding to specific enterocyte receptors for intestinal colonization and enterotoxins responsible for fluid secretion. The best-characterized adhesins are expressed in the context of fimbriae, such as the F4 (also designated K88), F5 (K99), F6 (987P), F17 and F18 fimbriae. Once established in the animal small intestine, ETEC produces enterotoxin(s) that lead to diarrhea. The enterotoxins belong to two major classes; heat-labile toxin that consist of one active and five binding subunits (LT), and heat-stable toxins that are small polypeptides (STa, STb, and EAST1). This chapter describes the disease and pathogenesis of animal ETEC, the corresponding virulence genes and protein products of these bacteria, their regulation and targets in animal hosts, as well as mechanisms of action. Furthermore, vaccines, inhibitors, probiotics and the identification of potential new targets identified by genomics are presented in the context of animal ETEC.

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“…46 The DNA templates for S100A11_UTR, S100A11_ORF and BC200 RNA were amplified using PCR with the corresponding primer pairs. RNAs were prepared by in vitro transcription using T7 RNA polymerase (Promega, P1320), with the amplified DNA fragments as templates.…”

Section: Electrophoretic Mobility Shift Assay (Emsa)mentioning

confidence: 99%

Knockdown of BC200 RNA expression reduces cell migration and invasion by destabilizing mRNA for calcium-binding protein S100A11

et al. 2017

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Although BC200 RNA is best known as a neuron-specific non-coding RNA, it is overexpressed in various cancer cells. BC200 RNA was recently shown to contribute to metastasis in several cancer cell lines, but the underlying mechanism was not understood in detail. To examine this mechanism, we knocked down BC200 RNA in cancer cells, which overexpress the RNA, and examined cell motility, profiling of ribosome footprints, and the correlation between cell motility changes and genes exhibiting altered ribosome profiles. We found that BC200 RNA knockdown reduced cell migration and invasion, suggesting that BC200 RNA promotes cell motility. Our ribosome profiling analysis identified 29 genes whose ribosomal occupations were altered more than 2-fold by BC200 RNA knockdown. Many (> 30%) of them were directly or indirectly related to cancer progression. Among them, we focused on S100A11 (which showed a reduced ribosome footprint) because its expression was previously shown to increase cellular motility. S100A11 was decreased at both the mRNA and protein levels following knockdown of BC200 RNA. An actinomycin-chase experiment showed that BC200 RNA knockdown significantly decreased the stability of the S100A11 mRNA without changing its transcription rate, suggesting that the downregulation of S100A11 was mainly caused by destabilization of its mRNA. Finally, we showed that the BC200 RNAknockdown-induced decrease in cell motility was mainly mediated by S100A11. Together, our results show that BC200 RNA promotes cell motility by stabilizing S100A11 transcripts.

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Identification of novel sRNAs involved in biofilm formation, motility and fimbriae formation in Escherichia coli

Cited by 91 publications

References 72 publications

Alternative pathways for Escherichia coli biofilm formation revealed by sRNA overproduction

Alternative pathways for Escherichia coli biofilm formation revealed by sRNA overproduction

Animal EnterotoxigenicEscherichia coli

Knockdown of BC200 RNA expression reduces cell migration and invasion by destabilizing mRNA for calcium-binding protein S100A11

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