Identification of Olea europaea L. cultivars using inter-simple sequence repeat markers

Terzopoulos, P.J.; Kolano, Bożena; Bebeli, Penelope J.; Kaltsikes, P. J.; Metzidakis, Ιoannis

doi:10.1016/j.scienta.2005.01.011

Cited by 41 publications

(37 citation statements)

References 13 publications

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“…The high level of polymorphism at ISSR loci also indicates high genetic variability in wild olive trees, which is in agreement with other studies made on cultivated (Gemas et al, 2004;Terzopoulos et al, 2005;Essadki et al, 2006;Gomes et al, 2009;Martins-Lopes et al, 2009) and wild olive trees (Hess et al, 2000).…”

Section: Discussionsupporting

confidence: 92%

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Noormohammadi

Samadi-Molayousefi²,

Sheidai³

2012

Genet. Mol. Res.

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ABSTRACT. Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 RAPDs and 10 ISSR markers. The mean value of polymorphism for RAPD loci was 73.71%, while the value for ISSR loci was 81.74%. The Keshar population had the highest value of intra-population polymorphism for both RAPD and ISSR loci (66.86 and 62.71%, respectively), while the Tudar population had the lowest values (20.35 and 28.81%, respectively). Similarly, the highest and lowest number of effective alleles, Shannon index and Nei's genetic diversity were also found for these two populations. The highest value of H pop /H sp within population genetic diversity for RAPD and ISSR loci was found for the Keshar population (H pop = 0.85 and H sp = 0.90). OPA04-750, OPA13-650 and OPA02-350 RAPD bands were specific for Tudar, Bondon and Keshar populations, respectively, while no specific ISSR bands were observed. Analysis of molecular variance as well as the pairwise F ST test showed significant differences for RAPD and ISSR markers among the populations. The NJ and UPGMA trees also separated the wild olive populations from each other, indicating their genetic distinctness. UPGMA clustering of the four wild olive populations placed the Tudar population far from the other populations; Keshar and Bokhoon population samples revealed more similarity and were grouped together. We conclude that there is high genetic diversity among O. europaea ssp cuspidata populations located in southern Iran. We also found RAPD and ISSR markers to be useful molecular tools to discriminate and evaluate genetic variations in wild olive trees.

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Section: Discussionsupporting

confidence: 92%

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Noormohammadi

Samadi-Molayousefi²,

Sheidai³

2012

Genet. Mol. Res.

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“…The high level of polymorphism observed in this study and in other reports of Portuguese (Gemas et al 2004;Lopes et al 2004) and foreign cultivars (Fabbri et al 1995;Weisman et al 1998;Barranco et al 2000;Belaj et al 2003a, b;Terzopoulos et al 2005) indicated that olive is a highly polymorphic species. The high diversity found between olive cultivars is probably due to a diverse germplasm origin, that presumably results from crosses Figure 1.…”

Section: Combined Phenetic Relationshipsupporting

confidence: 73%

“…ISSR technique is based on the amplification of regions (100-3,000 bp) between inversely oriented closely spaced microsatellites. ISSRs are PCR products obtained with primers based on dinucleotide, trinucleotide, tetranucleotide and pentanucleotide repeats (Zietkiewicz et al 1994), and have been used for olive cultivar identification (Hess et al 2000;Pasqualone et al 2001;Gemas et al 2004;Terzopoulos et al 2005).…”

Section: Introductionmentioning

confidence: 99%

RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification

Martins-Lopes

Lima-Brito

Gomes

et al. 2006

Genet Resour Crop Evol

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Thirty Portuguese and eight foreign olive (Olea europaea L.) cultivars were screened using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers. Twenty RAPD primers amplified 301 reproducible bands of which 262 were polymorphic; and 17 ISSR primers amplified 204 bands of which 180 were polymorphic. The percentage of polymorphic bands detected by ISSR and RAPD was similar (88 and 87%, respectively). The genetic variability observed was similar in the Portuguese and foreign olive cultivars. Seven ISSR and 12 RAPD primers were able to distinguish individually all 38 olive cultivars. Twenty specific molecular markers are now available to be converted into Sequence Characterised Amplified Region (SCAR) markers. Relationships among Portuguese and foreign cultivars is discussed.

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“…Similarly high polymorphic fragments were obtained by many workers in so many plants especially in olive, with varying results of 93% [22], 96% [23] when the gene analysis were done with ISSR-PCR system. An average mean of 1.6471 effective number of alleles (ne) were obtained from the average means of 1-9647 number of observed alleles (na).…”

Section: Jasmine and Balakrishnansupporting

confidence: 55%

Intra Specific Analysis of Gloriosa Superba (L) Through Issr Finger Printing and Dna Sequencing of Ecotypes Collected From Different Accessions of Tamil Nadu State, India

Jasmine

Balakrishnan²

2018

RIPB

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Diversity within and among the population of Gloriosa superba collected from five different location of Tamil Nadu State, India, were explored by using Inter Simple Sequence Repeat (ISSR) DNA sequencing method. A total of 86.27% of polymorphic bands were seen in 94 reproducible bands generated from 12 number of ISSR primer ranging between 200bp to1000bp of band width. ISSR primer produces the bands with an average polymorphism of more than ninety nine percent among all the ecotypes. The dendrograms drawn for the analysis of genetic similarity in all the ecotypes. Our studies reveals that, genetic variations among different accessions of Gloriosa superba(L) was very well identified with the help of ISSR finger printings technic. KEYWORDS: Dendrogram, Gloriosa superba, accessions, ecotypes, genetic variationCopyright: © 2018 The authors. This article is open access and licensed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted, use, distribution and reproduction in any medium, or format for any purpose, even commercially provided the work is properly cited. Attribution -You must give appropriate credit, provide a link to the license, and indicate if changes were made.

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Identification of Olea europaea L. cultivars using inter-simple sequence repeat markers

Cited by 41 publications

References 13 publications

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification

Intra Specific Analysis of Gloriosa Superba (L) Through Issr Finger Printing and Dna Sequencing of Ecotypes Collected From Different Accessions of Tamil Nadu State, India

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