Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro

Peng, Bo; Chen, Hanyang; Tan, Yulin; Jin, Meilin; Chen, Huanchun

doi:10.1007/s11427-006-0158-7

Cited by 13 publications

(9 citation statements)

References 20 publications

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“…Infectious bronchitis virus (IBV) is an enveloped coronavirus that contains an unsegmented, single-stranded and positive-sense RNA genome. The IBV genome encodes four major structural proteins, known as the spike (S) glycoprotein, the small envelope (E) protein, the membrane (M) glycoprotein, and the nucleocapsid (N) protein (Spaan et al, 1988;Peng et al, 2006). The spike protein is cleaved into S1 and S2, of which S1 produces neutralizing and serotype-specific antibodies and cell attachment determinants have been located to the S1 (Cavanagh et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Priming with a DNA vaccine and boosting with an inactivated vaccine enhance the immune response against infectious bronchitis virus

Wang

Tai

et al. 2010

Journal of Virological Methods

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The methods of repeated immunization with inactivated vaccines have been used widely to increase antibody protection against infectious bronchitis virus (IBV). However, compared with DNA vaccines, these methods usually induce poor cellular responses. In the present study, specific pathogen-free (SPF) chickens were immunized intramuscularly with a DNA vaccine carrying the main IBV structural genes (pVAX1-S1, pVAX1-M, and pVAX1-N, respectively) and boosted with the IBV M41 strain inactivated vaccine to assess whether such a new strategy could enhance the immune responses against IBV. The protection efficacy of the DNA vaccine carrying different structural genes for priming was evaluated further. The chickens were immunized primely on day 7 and boosted 2 weeks later. After that, distribution of the DNA vaccine in vivo, the percentage of CD4+CD3+ and CD8+CD3+ subgroups of peripheral blood T-lymphocytes, and the specific IgG and virus neutralizing antibodies were measured. Chickens were then challenged by the nasal-ocular route with the IBV M41 strain 4 weeks after booster immunization. The results demonstrated that priming with a DNA vaccine encoding nucleocapsid protein (pVAX1-N) and boosting with the inactivated IBV vaccine led to the dramatic augmentation of humoral and cellular responses, and provided up to 86.7% rate of immune protection, providing an effective approach to protect chickens from IBV.

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Section: Introductionmentioning

confidence: 99%

Priming with a DNA vaccine and boosting with an inactivated vaccine enhance the immune response against infectious bronchitis virus

Wang

Tai

et al. 2010

Journal of Virological Methods

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“…The S protein is post-translationally cleaved into the outer S1 and the membrane bound S2 proteins. S1 protein comprises major antigenic determinants that induce neutralizing Abs which makes it a major target for vaccine design and immune therapy [6][7][8][9]. S2 protein is conserved and comprises epitopes inducing cross-reactive Abs and cell-mediated immune (CMI) responses.…”

mentioning

confidence: 99%

The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens

Tian

Wang

et al. 2008

Biochemical and Biophysical Research Communications

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Epitope-based vaccines designed to induce cellular immune response and antibody responses specific for infectious bronchitis virus (IBV) are being developed as a means for increasing vaccine potency. In this study, we selected seven epitopes from the spike (S1), spike (S2), and nucleocapsid (N) protein and constructed a multi-epitope DNA vaccine. The 7-day-old chickens were immunized intramuscularly with multi-epitope DNA vaccine encapsulated by liposome and boosted two weeks later, and were challenged by virulent IBV strain five weeks post booster. The results showed that multi-epitope DNA vaccine led to a dramatic augmentation of humoral and cellular responses, and provided up to 80.0% rate of immune protection. The novel immunogenic chimeric multi-epitope DNA vaccine revealed in this study provided a new candidate target for IBV vaccine development.

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“…Several antiviral peptides in the form of specific virus ligands have recently been obtained by phage-display technology, including peptides for Newcastle disease virus (Chia et al, 2006), infectious bronchitis virus (Peng et al, 2006), hepatitis C virus (Hong et al, 2010), and human immunodeficiency virus (Welch et al, 2010). We therefore determined the antiviral activity of peptides 4, 5, and 6 using TCID 50 assay and real-time PCR and showed that all three peptides interfered with virus replication to some extent, though peptides 5 and 6 were more effective than peptide 4.…”

Section: Discussionmentioning

confidence: 99%

Characterization and utility of phages bearing peptides with affinity to porcine reproductive and respiratory syndrome virus nsp7 protein

Wang¹,

Liu²,

Cui³

et al. 2015

Journal of Virological Methods

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Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro

Cited by 13 publications

References 20 publications

Priming with a DNA vaccine and boosting with an inactivated vaccine enhance the immune response against infectious bronchitis virus

Priming with a DNA vaccine and boosting with an inactivated vaccine enhance the immune response against infectious bronchitis virus

The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens

Characterization and utility of phages bearing peptides with affinity to porcine reproductive and respiratory syndrome virus nsp7 protein

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