Identification of oral bacteria on titanium implant surfaces by 16S rDNA sequencing

Melo, Fabiana de; Nascimento, Cássio do; Souza, Diogo O.; Albuquerque, Rubens Ferreira de

doi:10.1111/clr.12865

Cited by 24 publications

(26 citation statements)

References 47 publications

(92 reference statements)

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“…Regarding bacterial diversity, it was only in the in- situ study by de Melo et al [9] that dental plaque samples were compared to SF-biofilm samples using 16S rRNA gene amplicon sequencing. However, these authors analysed 24-h biofilms and evaluated different substrates to those used in the present series (titanium discs vs supra/subgingival tooth surfaces from the same four subjects).…”

Section: Discussionmentioning

confidence: 99%

“…De Melo et al [9] detected that the 24-h biofilm obtained from titanium surfaces had 60% fewer OTUs than a biofilm obtained from tooth surfaces. In contrast, in our series, the SF-biofilm of the different materials (enamel, glass, and hydroxyapatite) had a bacterial richness and an evenness similar to that found in the TF-biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, the artificial substrates discussed in the literature are composed of different materials (glass, hydroxyapatite, enamel, or titanium) and have been held in different positions inside the oral cavity [4–9]. None of these artificial substrates have demonstrated clear advantages over other substrate types.…”

Section: Introductionmentioning

confidence: 99%

“…There are some studies in which 16S rRNA gene amplicon sequencing has been used to analyse the in- situ bacterial diversity of the SF-biofilm in several intraoral devices [9,12–14]. However, only one of them compared the diversity of the 24-h biofilm on titanium discs to that formed on the natural teeth [9].…”

Section: Introductionmentioning

confidence: 99%

“…However, only one of them compared the diversity of the 24-h biofilm on titanium discs to that formed on the natural teeth [9]. …”

Section: Introductionmentioning

confidence: 99%

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In situ substrate-formed biofilms using IDODS mimic supragingival tooth-formed biofilms

Tomás

Prada-López

Quintas

et al. 2018

Journal of Oral Microbiology

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This study aimed to compare the bacterial viability and diversity of a substrate-formed biofilm (SF-biofilm) in situ to a supragingival tooth-formed biofilm (TF-biofilm) in the same group of individuals. The impact of the device/disc position and toothbrushing during the formation of SF-biofilm was also assessed. Two tests were run. In test 1, 15 volunteers wore two hemi-splints carrying six discs of human enamel, glass, and hydroxyapatite for 2 days, and were instructed to not perform any oral hygiene measure. Biofilm samples were collected from the substrates and the contralateral tooth and were analysed using CLSM. In five volunteers, half of the biofilm present on the discs and their contralateral teeth were scraped and analysed using 16S pyrosequencing. In test 2, the microscopic analysis was repeated only on the SF-biofilm samples, and the volunteers were allowed to brush their teeth. Multivariate analyses revealed that the donors had a significant effect on the composition of the biofilm, confirming its subject-dependent character. The bacterial composition of the SF-biofilm was similar to the TF-biofilm, with significant differential abundance detected in very few taxa of low abundance. The toothbrushing during the formation of SF-biofilm was the only factor that conditioned the thickness or bacterial viability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…However, only one of them compared the diversity of the 24-h biofilm on titanium discs to that formed on the natural teeth [9]. …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In situ substrate-formed biofilms using IDODS mimic supragingival tooth-formed biofilms

Tomás

Prada-López

Quintas

et al. 2018

Journal of Oral Microbiology

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Effect of sucrose on biofilm formed in situ on titanium material

Souza

Cury

Filho

et al. 2018

Journal of Periodontology

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Short‐term effects of hyaluronic acid on the subgingival microbiome in peri‐implantitis: A randomized controlled clinical trial

Soriano‐Lerma

Magán‐Fernández

Gijón

et al. 2019

Journal of Periodontology

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BackgroundThe aim of our study was to evaluate the effects of a hyaluronic acid (HA) gel at 45 days on the microbiome of implants with peri‐implantitis with at least 1 year of loading.MethodsA randomized controlled trial was conducted in peri‐implantitis patients. Swabs containing the samples were collected both at baseline and after 45 days of treatment. 16S rRNA sequencing techniques were used to investigate the effect of HA gel on the subgingival microbiome.ResultsOne hundred and eight samples of 54 patients were analyzed at baseline and after follow‐up at 45 days. Three strata with different microbial composition were obtained in the samples at baseline, representing three main microbial consortia associated with peri‐implantitis. Stratum 1 did not show any difference for any variable after treatment with HA, whereas in stratum 2, Streptococcus, Veillonella, Rothia, and Granulicatella did decrease (P < 0.05). Similarly, Prevotella and Campylobacter (P < 0.05) decreased in stratum 3 after treatment with HA. Microbial diversity was found to be decreased in stratum 3 (P < 0.05) after treatment with HA compared with the control group, in which an increase was found (P < 0.05).ConclusionsHA reduced the relative abundance of peri‐implantitis‐related microorganisms, especially the early colonizing bacteria, suggesting a specific action during the first stages in the development of the disease. HA did not alter relative abundances of non‐oral genera. The use of HA in advanced stages of peri‐implantitis resulted in a decrease in microbial alpha diversity, suggesting a protective action of the peri‐implant site against bacteria colonization.

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Identification of oral bacteria on titanium implant surfaces by 16S rDNA sequencing

Abstract: Machined and SLA surfaces are colonized by similar numbers of prokaryotic OTUs after 24 h of exposure to the oral environment. Higher complexity of the titanium surface topography in the initial phase of biofilm maturation does not seem to significantly influence the colonizing microbiota.

Cited by 24 publications

References 47 publications

In situ substrate-formed biofilms using IDODS mimic supragingival tooth-formed biofilms

In situ substrate-formed biofilms using IDODS mimic supragingival tooth-formed biofilms

Effect of sucrose on biofilm formed in situ on titanium material

Short‐term effects of hyaluronic acid on the subgingival microbiome in peri‐implantitis: A randomized controlled clinical trial

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