Identification of outer membrane proteins of Edwardsiella tarda in response to high concentration of copper

Abstract

The antimicrobial properties of copper have been reported, but little is known about the outer membrane proteins regulating copper resistance. In the present study, a sub-proteomic approach was utilized to investigate altered outer membrane (OM) proteins of Edwardsiella tarda in response to CuSO4. Upregulation of HemR and dwonregulation of Imp, EvpB, TolC, ETAE_2935, ETAE_1480, and ETAE_1723 were detected in E. tarda EIB202 survived in 1.0 mM CuSO4 compared with the control without the ion. These alterations were validated, at random, using Western blotting. They were first revealed here to be bacterial copper-resistant proteins in combination of protein homology analysis. These findings highlight the way to clarify copper-resistant mechanisms