Identification of Panton-Valentine leukocidin virulence gene in methicillin—resistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran

Ranjkesh, Atefeh; Salari, Zahra; Nejat, Mehrab; Behboudi, Emad; Ramezani, Ali; Zandi, Milad; M, Marzban; Kiani, Hadis; Bavi, Mina; Roodsari, Mehdi Rabiei; Zibaee, Yousef Esvand; Abbasi, Samaneh; Elyasi, Maryam Mohammadi; Parsanahad, Mehdi; Jalilian, Shahram; Gorji, Simin Mohammadi; Owrang, Mina

doi:10.4103/2221-6189.283890

Cited by 2 publications

(2 citation statements)

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“…Fourteen of the 54 isolates of S. aureus tested positive for the PVL gene which is a prevalence of 25.9%. This is higher than the prevalence observed in Spanish study between 2005 and 2008 with prevalence of 3% [ 31 ] and 4.5% in Malaysia by Nastaly et al [ 32 ], as well as 20% observed by Owrang et al [ 33 ] in Iran and 24.1% prevalence reported in China by Wu et al [ 34 ]. The PVL prevalence observed in this study is also comparatively higher than those reported in some other African countries with Thabit et al [ 35 ] reporting 5.4% prevalence in Egypt and prevalence of 10.7% in Nigeria by Orji et al [ 13 ].…”

Section: Discussioncontrasting

confidence: 53%

Molecular Detection of Panton Valentine Leukocidin Toxin in Clinical Isolates of Staphylococcus aureus from Kiambu County, Kenya

Iliya

Mwangi

Maathai

et al. 2020

International Journal of Microbiology

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Panton–Valentine leukocidin gene is produced by Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus isolates as a pore-forming toxin is largely responsible for skin and soft tissue illnesses. MRSA produces PVL toxins through lukS and lukF proteins causing tissue necrosis by damaging membrane of the defense cells. Presence of PVL toxin was tested from the 54 S. aureus clinical isolates obtained from Thika and Kiambu Level 5 Hospitals, in Kiambu County, Kenya, by Geno Type® MRSA assay (Hain Life Science, Nehren, Germany). DNA was isolated from freshly harvested bacterial cultures by spin column using Geno Type DNA isolation kit. The detection of PVL toxins was performed by amplification of genomic DNA and by reverse hybridization that identifies PVL genes using Geno Type MRSA kit. Out of 138 samples that were collected from patients in Kiambu County, 54 S. aureus isolates were obtained, of which 14 (25.9%; 95% CI = 11.9–38.9) samples had PVL toxins. The isolates that were obtained from the female patients had a higher PVL toxin prevalence of 35.7%, while the isolates collected from the male patients had a lower prevalence of 15.4% (P=0.09). The pediatrics department had the highest PVL gene prevalence compared to outpatient department and surgical units (P=0.08). However, the age groups of patients and the hospital attended by patients showed no significant difference in terms of PVL gene prevalence (P=0.26). Therefore, the patients' gender and hospital units were not significantly associated with PVL gene prevalence (P=0.08). This study shows that PVL positive isolates occur in the sampled hospitals in the county and female as well as children must be taken into consideration among patients with wound infections when isolating S. aureus.

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Section: Discussioncontrasting

confidence: 53%

Molecular Detection of Panton Valentine Leukocidin Toxin in Clinical Isolates of Staphylococcus aureus from Kiambu County, Kenya

Iliya

Mwangi

Maathai

et al. 2020

International Journal of Microbiology

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“…Nineteen of the 30 coagulase-positive isolates tested positive for the PVL (lukS/F-PV)-encoding gene, giving a prevalence rate of 63.3%. This is the highest PVL prevalence rate compared to those observed in other Asian country studies: Iran prevalence of 20% (Ranjkesh et al, 2020) and 24.1% in China (Wu et al, 2019), as well as the ones reported in some African countries: Egypt prevalence of 16.1% (Thabit et al, 2017), Nigeria prevalence of 27% (Orji et al, 2016) and up to 61% in Gambia (Darboe et al, 2019).…”

Section: Discussionmentioning

confidence: 50%

Molecular differentiation of coagulase-positive staphylococcal isolates carrying mecA- and PVL-encoding genes among healthy males

Hanafy, A. M.,

Al-Jabri, K. R.,

Al-Ahmadi, R. A.

et al. 2024

MJM

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Aims: This study was aimed to monitor the asymptomatic carriage of coagulase-positive staphylococcal bacteria among university male students and detect the prevalence of virulence marker genes that encode methicillin resistance (mecA) and Panton-Valentine leukocidin (PVL) toxin among the isolates. Methodology and results: Single nasal swaps were collected from 144 participating students who resided at four different locations within Al-Madinah city. A total of 112 Gram-positive staphylococcal isolates were recovered from the 144 participants (carriage rate of 77.8%). Coagulase-positive staphylococci were differentiated using duplex PCR amplification of the 16S rRNA and nuc genes and accounted for 30 isolates (carriage rate of 20.8%). These isolates were most prevalent in the northern and southern parts of Al-Madinah city, while the lowest numbers of isolates were detected in students of the eastern part. Coagulase-positive isolates were further phenotypically characterized for methicillin resistance by the disc diffusion method. Uniplex PCR assays were conducted to screen for mecA-and PVL toxin-encoding genes. The mecA gene was amplified from all 15 (50%) methicillin-resistant coagulase-positive isolates, while the PVL toxin-encoding gene was detected in 19 isolates (63.3%), 10 (33.3%) of which contained the mecA gene. Lastly, PCR amplification of the NRPS gene from coagulase-positive isolates revealed the absence of Staphylococcus argenteus, the recently discovered genetically divergent lineage of Staphylococcus aureus. Conclusion, significance and impact of study: An elevated prevalence of coagulase-positive isolates harboring mecA and PVL virulence genes was observed compared with previous investigations. This poses a potential threat if they spread among the population, resulting in outbreaks of community-acquired infections.

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Identification of Panton-Valentine leukocidin virulence gene in methicillin—resistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran

Cited by 2 publications

References 1 publication

Molecular Detection of Panton Valentine Leukocidin Toxin in Clinical Isolates of Staphylococcus aureus from Kiambu County, Kenya

Molecular Detection of Panton Valentine Leukocidin Toxin in Clinical Isolates of Staphylococcus aureus from Kiambu County, Kenya

Molecular differentiation of coagulase-positive staphylococcal isolates carrying mecA- and PVL-encoding genes among healthy males

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