Identification of Pasteurella multocida transcribed genes in porcine lungs through RNAseq

Abstract

Pasteurella multocida is one of the most important pathogen that causes pneumonia in swine. Although several virulence factors are known, the pathogenesis of this bacterium is not well-studied. Therefore, to study the pathogenesis of P. multocida infection in porcine lung, next-generation RNA sequencing was used to compare the transcriptomes of P. multocida grown in vivo and in vitro, respectively. After P. multocida infection a total of 704 genes were expressed in vitro, 1422 genes were expressed in vivo, and 237 genes were differentially expressed based on statistical analyses, padj of ≤0.1. Genes encoding ribosomal proteins or other products that function in the regulation of transcription and translation were downregulated, whereas genes whose products affected cellular processes (protein transport and RNA degradation) and metabolic pathways, such as those of amino acid metabolism and nucleotide metabolism, were upregulated in vitro compared with in vivo. This study shows that differentially expressed genes in P. multocida regulate pathways that operate during stress, iron capture, heat shock, and nitrogen regulation. However, extensive investigation of the pathogenic mechanism of P. multocida is still required.

The Profiles of Long Non-coding RNA and mRNA Transcriptome Reveals the Genes and Pathway Potentially Involved in Pasteurella multocida Infection of New Zealand Rabbits

“…The characteristics of lncRNAs are similar to mRNA after being post-transcriptionally capped and polyadenylated ( 15 ). Unlike mRNA, which has been extensively investigated in P. multocida ( 35 , 36 ), the function of most lncRNAs and their potential roles in P. multocida infection are poorly explained. Based on the sequences, structures, and subcellular localization, lncRNAs display versatile functions of regulator gene expression splicing, nucleic acid degradation that interacts with DNA, RNA, and proteins ( 37 ).…”

Effect of conditioned media from Aeromonas caviae on the transcriptomic changes of the porcine isolates of Pasteurella multocida

“…It decreased the expression of genes involved in energy metabolism and electron transport (eno, pgk, and gapdh) [43]. During the in vitro growth, genes involved in protein transport, RNA degradation, amino acid metabolism, and nucleotide metabolism were highly expressed compared to the in vivo study [38]. These transcriptomic profiles provided molecular snapshots of how P. multocida responded to the in vivo and in vitro environments.…”

“…Several studies suggested that the interaction between members in the microbiome could promote host wellness by enhancing harvest, storage, energy metabolism [33,34], and immune regulation [35,36]. One common approach for examining these molecular mechanisms of the bacteria-host interactions was the high-throughput gene expression assay or transcriptomics by RNA sequencing (RNASeq) [37,38]. For example, on the host response, the transcriptomic analysis of P. multocida-infected murine lungs found the upregulated genes related to immune responses, i.e., pattern recognition receptors (PRRs), chemokines, and inflammatory cytokines in the Il-and IFN-families [39].…”

Draft Genome Sequence of Pasteurella multocida Serotype A Strain MOR19, Isolated in Morocco

“…The importance of Pasteurella multocida is well established as an etiologic factor of hemorrhagic septicemia and pneumonia in cattle and sheep, which is a major cause of economic loss and mortality. The disease complex seems to be caused by a combination of respiratory viruses, bacterial infections, stress, and other environmental factors ( 2 ).…”