Identification of Pathogens Associated with Bulb Rot of Lily during Storage and Effects of Bulb Disinfection on Development of Lily Bulb Rot

Hahm, Soo-Sang; So-Young, Oh; Lee, Eun-Mo; Yu, Shao-Ming

doi:10.5423/rpd.2006.12.1.020

Cited by 4 publications

(2 citation statements)

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“…Isolates of T. adpressus, P. gladioli, T. calidominioluteus, and P. polonicum were obtained from the naturally occurring blue mold of lily. Hahm et al [25] identified several kinds of postharvest disease of lily bulbs during storage, and, in stabbing experiments, Penicillium spp. and Fusarium spp.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Pathogens Causing Blue Mold of Lanzhou Lily during Postharvest Storage and Control of Disease and Mycotoxin Accumulation by Ozone Treatment

Zhang,

Xi,

Liu

et al. 2023

JoF

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Blue mold (penicilliosis) is a common disease of Lanzhou lily (Lilium davidii var. willmottiae) during postharvest storage, which not only seriously affects the appearance and reduces the quality of lily bulbs, but also leads to the accumulation of mycotoxins in rotten lily tissues, seriously endangering human health. Therefore, it is of great significance to clarify the main isolates causing postharvest blue mold of fresh Lanzhou lily and put forward effective measures to control the disease caused by these pathogens. In this study, pathogens were isolated and purified from the naturally diseased blue-mold tissue of Lanzhou lily, and then morphological and molecular biology techniques were applied to identify the isolates, verify the pathogenicity, determine the disease index and disease incidence, and finally, to analyze the control effect of ozone treatment on the blue mold of lily scale and mycotoxin accumulation. The results indicated that the main isolates causing postharvest blue mold of lily were Talaromyces adpressus, Penicillium gladioli, T. calidominioluteus, and P. polonicum. The pathogenicity test showed that P. gladioli and P. polonicum had a higher disease index than T. calidominioluteus and T. adpressus. Ozone treatment significantly reduced the incidence of disease caused by P. gladioli and P. polonicum, and effectively controlled the accumulation of patulin. This study characterized the main pathogens causing blue mold of postharvest Lanzhou lily during storage, and confirmed ozone application has a significant inhibitory effect on blue mold development and patulin accumulation in Lanzhou lily, which could be helpful in commercially controlling blue mold of postharvest Lanzhou lily during storage.

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Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Pathogens Causing Blue Mold of Lanzhou Lily during Postharvest Storage and Control of Disease and Mycotoxin Accumulation by Ozone Treatment

Zhang,

Xi,

Liu

et al. 2023

JoF

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“…한 병원균의 포자가 대부분 격막이 없고, 난형 또는 콩팥모 양이었으며, 크기가 5-10 × 2-5µm로 소형분생포자로 형성 되어(Fig.1) 접종하기 가장 적합하다고 판단하였다(Ham et al, 2006).Straathof and Van Tuyl(1994)은 백합 구근부패병 에 대한 식물체의 반응을 6단계로 나눈 바 있으며 그 기준에 따라 강한 저항성, 중간 저항성, 민감성으로 품종들을 분류 하였고, 이에 따라 본 실험에서는 Fol균 접종 후 나타나는 발병 지수를 6단계로 나누어 지수 1은 구근부패병의 증상이 없이 대조구와 비슷한 수준, 지수 2는 잎 절편체의 가장 자 리에서 갈변의 시작이 보이는 경우, 지수 3은 잎의 중앙 부 분까지 갈변이 진행되고 일부 고사가 보이는 경우, 지수 4는 잎의 전반적인 면이 갈변되면서 부패 증상이 나타나기 시작 수가 대조구와 비슷하게 지수 1정도였고, 7일 후엔 잎의 반 정도가 고사가 진행되어 발병 지수 3을 나타냈다. 1.0 × 10 4 spores･mL -1 , 1.0 × 10 5 spores･mL -1 의 농도에서는 초기부터 3이상의 발병 지수를 나타내었다(Fig.…”

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Development of an Efficient In Vitro Screening Method for Selection of Resistant Lily Cultivars Against Fusarium oxysporum f. sp. lilii

Jang¹,

Moon²,

Ha³

et al. 2015

HST

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The soilborne fungus Fusarium oxysporum f. sp. lilii (Fol) is a serious threat to all lily cultivars, especially infecting bulbs and flowers. It has become increasingly important to develop varieties resistant against the bulb rot disease. Genetic diversity of cultivars and reliable screening methods are required for this purpose. Here, an efficient in vitro screening system for evaluating resistance to Fol in 38 in vitro-grown lily plants was investigated. Various factors including culture conditions of Fol, inoculum density, appropriate plant materials, inoculation method and duration, and incubation period of plant materials after inoculation were combined to optimize the screening method. As a result, we suggest optimal conditions for an in vitro screening system for the selection of Fol-resistant lily cultivars as follows. Fol was grown on potato dextrose agar (PDA) medium for 6 days at 25°C in darkness and used as working inoculation. Spore suspensions were prepared (inoculum density: 1.0 × 10 4 spores‧mL -1), and then leaf segments 1.5 × 2.0 cm 2 were inoculated by dipping for 22 hours at 25°C in dark. Later, leaves were cultured on 0.6% agar plates at 25°C and 50% humidity with a photoperiod of 16 hours light/8 hours dark (fluence rate of 40 µmol･m) to examine the progress of bulb rot. After 7 days, disease levels were classified into indices 1 (no symptom) to 6 (serious bulb rot). Soil inoculation of Fol carried out with resistant or susceptible lily cultivars that had been selected through in vitro screening confirmed the reproducibility of results. Therefore, the in vitro screening method established in this study is efficient and reliable for selection of lily cultivars resistant against bulb rot disease.

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First Report of Bulb Rot on Lily Caused by Curvularia pseudobrachyspora in China

Zeng

et al. 2020

Plant Disease

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Identification of Pathogens Associated with Bulb Rot of Lily during Storage and Effects of Bulb Disinfection on Development of Lily Bulb Rot

Cited by 4 publications

References 1 publication

Isolation and Identification of Pathogens Causing Blue Mold of Lanzhou Lily during Postharvest Storage and Control of Disease and Mycotoxin Accumulation by Ozone Treatment

Isolation and Identification of Pathogens Causing Blue Mold of Lanzhou Lily during Postharvest Storage and Control of Disease and Mycotoxin Accumulation by Ozone Treatment

Development of an Efficient In Vitro Screening Method for Selection of Resistant Lily Cultivars Against Fusarium oxysporum f. sp. lilii

First Report of Bulb Rot on Lily Caused by Curvularia pseudobrachyspora in China

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