Identification of pathotypes of <i>Synchytrium endobioticum</i> found in infested fields in Turkey

Çakir, Emel; Leeuwen, G. C. M. van; Flath, Kerstin; Meffert, Johan P.; Janssen, Werner A.; Maden, Salih

doi:10.1111/j.1365-2338.2009.02285.x

Cited by 26 publications

(16 citation statements)

References 5 publications

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“…3). Potato sprouts were analyzed under a microscope BioLight 300 (DELTA optical, Poland) to determine the degree of damage according to the following scale, adapted after [13,16]:…”

Section: Methodsmentioning

confidence: 99%

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2018

Agric. sci. pract.

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Aim.To evaluate potato breeding material for resistance to pathotypes of Synchytrium endobioticum (Schilbersky) Percival (1909) known to be present in Ukraine (pathotypes 1(D 1 ), 11, 13, 18 and 22); to identify resistant registered and potential varieties for the usage in the national wart disease eradication programs and to recommend these selected (potential and registered) potato varieties for the breeding program targeted on the development of multiple resistance against pathotypes of S. endobioticum present in Ukraine. Methods. Evaluation of the potato breeding material and registered potato varieties for the resistance against common pathotype 1 (D 1 ) and four aggressive pathotypes of S. endobioticum (pathotypes 11, 13, 18 and 22) in climatic chamber and greenhouse tests of Ukrainian Scienti¿ c Research Plant Quarantine Station of Institute of Plant Protection NAAS (Boyany, Ukraine) following the Spieckermann and Glynne-Lemmerzahl methods (EPPO Standard PM7/28 (2)). Field trials on naturally infected soils were conducted according to standard methods adapted to national requirements in the area of Chernivtsi, Zakarpattia and Ivano-Frankivsk regions. Results. 3,736 samples of potato breeding material from six breeding institutions of Ukraine were tested for resistance against S. endobioticum during 2011-2017 in the western region of the country. Among all samples tested, 3,389 were identi¿ ed as resistant to the widely spread pathotype 1 in the preliminary climatic chamber and greenhouse tests, and 130 of them proved to be resistant under ¿ eld conditions. Five out of 41 Ukrainian registered potato varieties (Bazys, Hlazurna, Solokha, Bozhedar and Santarka) were found to be resistant to all 5 pathotypes tested (1 (D 1 ), 11, 13, 18 and 22). Conclusions. The 130 samples of potato breeding material (which were found to be resistant against the common pathotype 1 of S. endobioticum in the laboratory, greenhouse as well as in the ¿ eld trials) were recommended for the state variety registration and further usage in an eradication program to localize potato wart outbreaks of the western part of Ukraine. The screening tests revealed that the national breeding program targeted on resistance against S. endobioticum pathotype 11 was the most effective (49 % of samples tested proved to be resistant against this pathotype), whereas it was the least effective against pathotype 18, namely only 30 % of samples resistant. It was speculated that such a dissimilarity may be related to the differences in the genetic material used in the breeding process at various institutions, and which may be the subject of further analysis in order to improve the results of breeding programs. The already registered potato varieties Bazys, Hlazurna, Solokha, Bozhedar and Santarka which were found to have a multiple resistance to common pathotype 1 and four local aggressive pathotypes of S. endobioticum (11, 13, 18 and 22) were recommended for use in the breeding process as sources of resistance and also for the eradication programs in ...

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“…3). Potato sprouts were analyzed under a microscope BioLight 300 (DELTA optical, Poland) to determine the degree of damage according to the following scale, adapted after [13,16]:…”

Section: Methodsmentioning

confidence: 99%

Untitled

2018

Agric. sci. pract.

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“…None of them matched any of the western European, Czech and Ukrainian pathotypes. The 14 isolates corresponded to seven new distinct pathotypes (Çakır et al 2009 ). Khiutti et al ( 2012 ) studied the resistance reaction of a well-characterized subset of the collection at the Vavilov Institute of Plant Industry in Russia.…”

Section: Resistance Breeding and S Endobioticum Pmentioning

confidence: 99%

Managing potato wart: a review of present research status and future perspective

2014

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Key messageIdentification of resistance genes to potato wart disease caused bySynchytrium endobioticumis the key for developing diagnostic markers for breeding resistant cultivars. We present an overview on the current knowledge of this host-pathogen system and molecular advances while highlighting future research focus.AbstractPotato wart is a quarantined disease of cultivated potato (Solanum tuberosum L.) caused by the obligate biotrophic, soil-borne fungus Synchytrium endobioticum (Schilb.) Perc. Since its discovery by Schilberszky in 1896, the management of wart disease was enabled by research efforts focusing on understanding and classifying the causative agent, its mode of infection, pathogenesis, geographical distribution, detection and chemical control, on developing screening methods for host resistance and on genetic analyses, which led to the development of resistant cultivars. These early successes are currently challenged by new S. endobioticum pathotypes evolving and the increased risk of dissemination by potato tuber trade. New research efforts are therefore required to ensure continuation of effective and sustainable management of the potato wart disease. Advances in molecular biology and genomic tools offer potential for innovations. This review presents an overview on what we know about this complex host-pathogen interaction, highlights recent molecular work and embarks on an outlook towards future research directions.

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“…Strict phytosanitary measures and the breeding and cultivation of resistant cultivars are the only feasible strategies for controlling potato wart disease. More than 40 different pathotypes of this pathogen have been reported (Baayen et al 2006 ; Çakır et al 2009 ; Przetakiewicz 2015 ), from which pathotypes 1, 2, 6, 8 and 18 are considered the most important forms of the fungus (Stachewicz 2002 ). Pathotype 18 is also considered to be among the most aggressive pathotypes, and only a few resistance genes have been characterized thus far.…”

Section: Introductionmentioning

confidence: 99%

Improved genetic resolution for linkage mapping of resistance to potato wart in monoparental dihaploids with potential diagnostic value in tetraploid potato varieties

et al. 2018

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Key messageWe achieved improved mapping resolution of the major wart resistance locus Xla-TNL containing also Sen1 in a dihaploid population using SNP data and developed additional markers with diagnostic value in tetraploid varieties.AbstractWe analyzed a segregating monoparental dihaploid potato population comprising 215 genotypes derived from a tetraploid variety that is highly resistant to Synchytrium endobioticum pathotypes 18 and 6. The clear bimodal segregation for both pathotypes indicated that a major dominant resistance factor in a simplex allele configuration was present in the tetraploid donor genotype. Compared to that in previous analyses of the same tetraploid donor in conventional crosses with susceptible tetraploid genotypes, a segregation pattern with a reduced genetic complexity of resistance in dihaploids was observed here. Using the 12.8 k SolCAP SNP array, we mapped a resistance locus to the Xla-TNL region containing also Sen1 on potato chromosome 11. The improved mapping resolution provided by the monoparental dihaploids allowed for the localization of the genes responsible for the resistance to both pathotypes in an interval spanning less than 800 kbp on the reference genome. Furthermore, we identified eight molecular markers segregating without recombination to pathotype 18 and pathotype 6 resistance. Also, two developed markers display improved diagnostic properties in an independent panel of tetraploid varieties. Overall, our data provide the highest resolution mapping of wart resistance genes at the Xla-TNL locus thus far.Electronic supplementary materialThe online version of this article (10.1007/s00122-018-3172-9) contains supplementary material, which is available to authorized users.

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Identification of pathotypes of Synchytrium endobioticum found in infested fields in Turkey

Cited by 26 publications

References 5 publications

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Managing potato wart: a review of present research status and future perspective

Improved genetic resolution for linkage mapping of resistance to potato wart in monoparental dihaploids with potential diagnostic value in tetraploid potato varieties

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