Identification of Pathways of A498 Human Kidney Carcinoma Cell Death under the Action of Gratiola officinalis L. Extract and Green Tea Flavonoids Using Fluorescence Imaging Techniques

Mylnikov, Artyom M.; Polukonova, N. V.; Isaev, D. S.; Doroshenko, A. A.; Verkhovskii, Roman A.; Nikolaeva, N. A.; Mudrak, D. A.; Navolokin, Nikita A.

doi:10.1134/s0030400x20070139

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…It is conditioned by the fact that under prolonged use of cytostatic drugs, the tumor cells develop resistance to their action, the morphological manifestation of which is the formation of autophagosomes. In our earlier studies, we obtained data confirming the ability of free extract of Gratiola officinalis to block the development of autophagy in tumor cells [ 53 , 54 ], which is consistent with the literature data about the interaction between autophagy and apoptosis [ 10 , 16 , 21 ].…”

Section: Discussionsupporting

confidence: 90%

Antitumor Effects of Microencapsulated Gratiola officinalis Extract on Breast Carcinoma and Human Cervical Cancer Cells In Vitro

et al. 2023

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Flavonoid-containing Gratiola officinalis extract has been studied in relation to breast carcinoma and human cervical cancer cells in encapsulated and native form. Encapsulation was realized in polymer shells, which were formed by the layer-by-layer method using sequential adsorption of poly(allylamine hydrochloride) and poly(sodium 4-styrenesulfonate) on the destructible cores. The extract was prepared by the author’s method and characterized using high performance liquid chromatography. By means of optical and fluorescent microscopy, cell changes under the action of pure and encapsulated extracts were comprehensively studied, and statistical analysis was carried out. Cells were stained with propidium iodide, acridine orange, and Hoechst 33258. A fluorescence microscope with a digital video camera were used for cell imaging. The encapsulated extract caused 100% death of breast cancer SKBR-3 cells and 34% death of cervical cancer HeLa cells and prevented the formation of autophagosomes in both cultures. Analysis of the viability and morphological features of tumor cells under the action of microencapsulated extract allows us to consider microencapsulation as an effective strategy for delivering Gratiola officinalis extract to tumor cells and a promising way to overcome the protective autophagy.

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Section: Discussionsupporting

confidence: 90%

Antitumor Effects of Microencapsulated Gratiola officinalis Extract on Breast Carcinoma and Human Cervical Cancer Cells In Vitro

et al. 2023

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“…Так, под действием индол-3-карбинола через 48 h количество клеток рака почки в апоптозе возрастало в 6.8−10 раз по сравнению с контролем. Противоопухолевая активность в отношении клеток рака почки индол-3-карбинола сходна с воздействием экстракта аврана лекарственного [6], однако даже превосходит его по силе воздействия. Полученные нами данные вполне согласуются с известными ранее для индол-3-карбинола в том, что он обладает противоопухолевой активностью за счет остановки пролиферации опухолевых клеток и индукции в них апоптоза [14,15,21].…”

Section: Discussionunclassified

“…В настоящее время известно, что перспективными противоопухолевыми средствами являются лекарственные средства, содержащие биофлавоноиды, обладающие широким спектром биологической активности, в том числе и способностью активировать апоптоз. Так, катехин (галлат эпигаллокатехин) экстракта зеленого чая обладает тремя основными механизмами противоопухолевой активности: антипролиферативным механизмом, торможением фазы G1 подготовки клетки к делению, механизмом индукции апоптоза [4][5][6]. Экстракт аврана лекарственного, полученный способом, позволяющим снижать токсичность и повышать выход флавоноидов из растительного сырья, обладает противоопухолевым, антикахетическим и иммуномодулирующим действиями.…”

Section: Introductionunclassified

Оценка Эффективности Противоопухолевого Воздействия И Индукции Апоптоза В Клетках Карциномы Почки Человека Биологически Активными Добавками, Содержащими Ресвератрол, Индол-3-Карбинол И Кордицепин, Флуоресцентными Методами Визуализации

Полуконова¹,

Исаев²,

Мыльников³

et al. 2021

Оптика И Спектроскопия

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We compared the effectiveness of antitumor effects and apoptosis induction on human renal cell carcinoma A498 extracts of cruciferous plant material (indole-3-carbinol), Chinese mushroom (cordycepin), French red wine (resveratrol) at low concentrations after 24 and 48 h using fluorescent methods of imaging apoptosis and necrosis in human tumor cells in vitro . Propidium iodide and acridine orange were used as dyes in the "alive and dead" test, which allowed us to detect the number of dead cells and cells in which apoptosis had started. It was found that indole-3-carbinol at low concentrations (0.0288 and 0.1152 mg/ml) has a pronounced cytotoxic and cytostatic activity against human kidney cancer cells, significantly exceeding the action of resveratrol at the same concentrations. At the same time, cordycepin has no cytotoxic and cytostatic activity at these concentrations. Indole-3-carbinol also has the most pronounced apoptotic activity at concentrations of 0.0144-0.1152 mg/ml, after 48 h the number of kidney cancer cells in apoptosis increases by 6.8-10 times compared to control. It is concluded that indole-3-carbinol is a promising antitumor agent for use in the complex therapy of patients with kidney cancer.

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Assessment by the Fluorescence Imaging Methods of the Antitumor Efficacy and Apoptotic Activity of Biologically Active Additives Containing Resveratrol, Indole-3-Carbinol, and Cordycepin in Human Renal Carcinoma Cells

et al. 2021

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Identification of Pathways of A498 Human Kidney Carcinoma Cell Death under the Action of Gratiola officinalis L. Extract and Green Tea Flavonoids Using Fluorescence Imaging Techniques

Cited by 3 publications

References 16 publications

Antitumor Effects of Microencapsulated Gratiola officinalis Extract on Breast Carcinoma and Human Cervical Cancer Cells In Vitro

Antitumor Effects of Microencapsulated Gratiola officinalis Extract on Breast Carcinoma and Human Cervical Cancer Cells In Vitro

Assessment by the Fluorescence Imaging Methods of the Antitumor Efficacy and Apoptotic Activity of Biologically Active Additives Containing Resveratrol, Indole-3-Carbinol, and Cordycepin in Human Renal Carcinoma Cells

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