Identification of PDC-109-like protein(s) in buffalo seminal plasma

The study was designed to evaluate the influence of season on semen characteristics and seminal plasma protein profile of buffalo bull semen. Thirty-six ejaculates were collected in three seasons (winter, summer and rainy) from six adult Bhadawari bulls, and semen characteristics were evaluated immediately after collection. The seminal plasma was harvested by centrifugation and protein profiling, and percentage protein fractions were analysed by SDS-PAGE. The significant effect of season was observed on ejaculate volume, sperm concentration, progressive motility, percentage live spermatozoa, hypo-osmotic swelling test (HOST) and acrosomal integrity. The electrophoretogram of seminal plasma proteins revealed 20 protein bands in winter, 23 bands in rainy and 25 bands in summer seasons, illustrating the significant effect of seasons on seminal plasma proteins. Among these protein bands, 18 bands were observed common in semen samples of all three seasons while protein bands of 46, 55, 58, 144 and 160 kDa were found in rainy and summer seasons. The protein bands of 48 and 60 kDa were observed only in winter season, whereas 184 and 200 kDa were reported in summer season only. The protein fractions (protein%) of common protein bands observed in three seasons revealed a significant effect of season on protein bands of 24.5, 66, 70, 72, 84 and 86 kDa. From the study, it was pertinent that bull seminal plasma contains specific proteins in particular season, which may be associated with some of the semen characteristics, and these proteins could be used as markers of the semen quality of buffalo bulls.

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“…The findings of the present study is in partial agreement with the reports of Harshan et al. (), Asadpour et al. () and Arangasamy et al.…”

Section: Discussionsupporting

confidence: 94%

“…() and Harshan et al. () may be due to difference in protein extraction procedure applied prior to SDS‐PAGE fractionation.…”

Section: Discussionmentioning

confidence: 99%

Seasonal Variations in Seminal Plasma Proteins of Buffalo

Sharma

Pandey

Nigam

et al. 2014

Reprod Domestic Animals

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“…Harshan et al (2009) reported percentage of isolated GB-and HB-protein fraction of cattle semen over TP as 32.2 and 31.8%, respectively; whereas in seminal plasma of Murrah buffalo bulls, HBPs and TP were reported as 6.6 ± 0.13 and 32.9 ± 1.5 mg/mL, respectively. Group of HBPs (14)(15)(16)(17)(18) are the same as BSP proteins (Therien et al, 1995).…”

Section: Bovine Seminal Plasma Proteinsmentioning

confidence: 90%

Bovine seminal PDC-109 protein: An overview of biochemical and functional properties

Srivastava

Adnot

Srivastava

et al. 2013

Animal Reproduction Science

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“…BSP protein homologs in SP of rams (RSP‐14, RSP‐16, RSP‐20, and RSP‐24 kd—30% of total SP proteins; Bergeron et al, 2005; Jobim et al, 2005) and goats (GSP‐14, GSP‐15, GSP‐20, and GSP‐22 kd—20% of total SP proteins; Villemure et al, 2003) also share heparin and phosphorylcholine‐binding properties. Very recently, Harshan et al (2009) have confirmed the presence of PDC‐109 in buffalo SP. In goat, GSP‐20 and GSP‐22 show a strong heparin affinity, whereas GSP‐14 and GSP‐15 interact weakly (Villemure et al, 2003).…”

Section: Biochemical Constituents Of Sp and Their Functionsmentioning

confidence: 91%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

224

169

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The biochemical composition of seminal plasma (SP) is very complex and variable among species. Advances in reproductive technologies reveal SP as a nutritive-protective medium for sperm cells suspended in it, and some SP components are very important for sperm metabolism, as well as sperm function, survival, and transport in the female reproductive tract. Biochemistry of SP is a relatively modern but rapidly expanding field of research, particularly concerning the biological significance of the various biochemical constituents of SP. However, identification of active factors in SP and mechanisms by which they act are not sufficiently understood in different species. Especially in camelids, the origin, composition, and function of the viscous component of SP remain a mystery, and only some biochemical and morphological characteristics of SP have been described. In this review, we focus on the current understanding of physiological and biochemical properties of SP in ruminants and camelids.

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Identification of PDC-109-like protein(s) in buffalo seminal plasma

Cited by 24 publications

References 19 publications

Seasonal Variations in Seminal Plasma Proteins of Buffalo

Seasonal Variations in Seminal Plasma Proteins of Buffalo

Bovine seminal PDC-109 protein: An overview of biochemical and functional properties

Seminal Plasma: An Essential Attribute to Spermatozoa

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