Identification of peptides, metal binding and lipid peroxidation activities of HPLC fractions of hydrolyzed oat bran proteins

Baakdah, Morooj M.; Tsopmo, Apollinaire

doi:10.1007/s13197-016-2341-6

Cited by 38 publications

(40 citation statements)

References 28 publications

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“…Some multifunctional hydrolysates with antioxidant, ACE, α‐glucosidase, or dipeptidyl peptidase‐IV inhibitory properties have been obtained from pea, eggs, and milk (Asoodeh, Homayouni‐Tabrizi, Shabestarian, Emtenani, & Emtenani, ; Li & Aluko, ; Zambrowicz et al, ). In addition to antioxidant and enzyme inhibitory activities, multifunctional protein hydrolysates can enhance the absorption of divalent mineral such calcium and iron due to their chelating ability (Baakdah & Tsopmo, ). Although tomato seeds are high in proteins, data are lacking on the multifunctionality of their hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical, antioxidant, calcium binding, and angiotensin converting enzyme inhibitory properties of hydrolyzed tomato seed proteins

et al. 2018

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The objective of this was to determine the impact of enzymatic hydrolysis on the multifunctionality of tomato seed protein hydrolysates (TSPH) and their physicochemical properties. The enzymatic hydrolysis was performed using alcalase and two factors response surface methodology. The best conditions were 131.4 min and 3% enzyme/substrate (E/S) for antioxidant activity; 174.5 min and 2.93% E/S for angiotensin‐converting enzyme (ACE) inhibition; and 66.79 min and 2.27% E/S for the calcium binding. Antioxidant and ACE hydrolysates were characterized by higher solubility, zeta potential, and thermal stability while properties of the calcium binding hydrolysate were only minimally affected by the enzymatic hydrolysis. Gel electrophoresis showed that molecular weights of polypeptides in the calcium binding TSPH were higher compared to those in ACE and antioxidant TSPHs. This was due to the low degree of hydrolysis of the calcium binding hydrolysate. Practical applications Nowadays, different protein sources are used to produce protein hydrolysates containing bioactive peptides that can help alleviate oxidation of foods, oxidative stress, and chronic conditions (e.g., hypertension, diabetes, cardiovascular disorder). Hydrolyzed proteins also have the potential to increase mineral absorption through the formation of mineral‐binding complexes. Biological activities of proteins and peptides from tomato processing byproduct (i.e., pomace) have received until now little attention. The determination of physicochemical properties and biological activities of the hydrolyzed proteins has application in the formulation of value‐added food products for the reduction of oxidative stress and risks of developing chronic diseases. In addition, there will be a reduction of pomace waste generated by the tomato processing industry.

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Section: Introductionmentioning

confidence: 99%

Physicochemical, antioxidant, calcium binding, and angiotensin converting enzyme inhibitory properties of hydrolyzed tomato seed proteins

et al. 2018

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“…Casein phosphopeptides (CPPs), for instance, derived from milk protein have been confirmed to chelate with calcium, zinc and iron and promote the mineral absorption both in vitro and in vivo (Hansen et al ., ; Kibangou et al ., ; García‐Nebot et al ., , , ). Besides, soy bean, oat bran, wheat germ, oyster and whey protein are all confirmed as food protein sources of chelating peptide (Lv et al ., ; Baakdah & Tsopmo, ; Cruz‐Huerta et al ., ; Udechukwu et al ., ). The influence of metal‐chelating peptides on mineral transport and the potential mechanism have been extensively investigated by in vivo animal and human studies along with in vitro cell culture models (Guo et al ., ).…”

Section: Introductionmentioning

confidence: 99%

The chelating peptide (GPAGPHGPPG) derived from Alaska pollock skin enhances calcium, zinc and iron transport in Caco‐2 cells

Chen

Guo

et al. 2017

Int J of Food Sci Tech

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Summary Gly‐Pro‐Ala‐Gly‐Pro‐His‐Gly‐Pro‐Pro‐Gly (GPAGPHGPPG), a chelating peptide derived from Alaska pollock skin, has been approved with outstanding ability to chelate calcium, zinc and iron ions. In this study, the stability of GPAGPHGPPG during in vitro gastrointestinal enzymatic digestion, the potential binding site between peptide and metal ions as well as its effects on mineral transport in Caco‐2 cells were investigated. Results showed that approximately 75% of GPAGPHGPPG remained intact at the end of the in vitro gastrointestinal enzymatic digestion. The formation of peptide‐metal complex was potentially related to histidine and cyclic structure in terms of dehydration. Moreover, GPAGPHGPPG revealed significant promotional effects on calcium (112.7%, P < 0.01), zinc (32.3%, P < 0.01) and iron (27.7%, P < 0.01) transport in Caco‐2 cell monolayer. In conclusion, GPAGPHGPPG could be developed as a potential functional ingredient to prevent mineral deficiency.

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“…The reaction rate of pyrogallol autoxidation decreases in the presence of O 2 ─• scavenger. Data (Figure 18) OBPIs and OBPHs in this study is similar to that obtained from a research by (Baakdah & Tsopmo, 2016), where the activity of GSH is much higher than their protein isolate and hydrolysate samples.…”

Section: Superoxide Radical (O 2 ─• ) Scavenging Activitysupporting

confidence: 88%

“…Environmental and occupational exposure to metals have been associated with the development of inflammations, cellular damage and several cardiovascular diseases, especially lung and skin cancer (Leonard et al, 2004). Carcinogenesis caused by metals has been previously investigated by different researchers, but the mechanism of action of metals especially at molecular level are still inconclusive (Baakdah & Tsopmo, 2016;Z. Fang et al, 2014;Navas-Acien et al, 2005).…”

Section: Metal Toxicity and Oxidative Stressmentioning

confidence: 99%

Effects of Hydrolyzed Oat Bran Proteins on Free Radicals, Toxic Metals and Lipid Oxidation

Shittu¹

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Transition metals play an important role in wide variety of biological processes in living system. Exposure to toxic metals, specifically arsenic (As) and chromium (Cr), has been associated with inflammation, cellular damage, and cardiovascular diseases as a result of As/Cr-mediated oxidative stress. In recent years, food proteins have been of major interest because in addition to being sources of essential amino acids they can also provide bioactive peptides. Several studies have found that these food peptides exhibit antioxidant, anti-inflammatory and anti-hypertensive properties. Oat is the sixth consumed type of cereal and its use in human nutrition has been increasing in recent years because of the discovery of functional and biological activity of some of its constituents. In the present study, medium oat bran was pre-treated with cellulase and viscozyme to degrade the carbohydrase, followed by the hydrolysis of the isolated proteins with proteases; alcalase (AL), papain (PA), flavourzyme (FL), and protamex (PRO). We investigated the characteristics of the oat bran protein isolates (OBPI) and the oat bran protein hydrolysates (OBPH), their ability to reduce the toxicity of arsenic and chromium as well as their radical scavenging properties. OBPI samples include cellulase (CELL) and viscozyme (VISC) pre-treated protein isolates respectively. OBPH samples were named based on the carbohyrase and protease used in hydrolysis; CAL, CPA, CFL, CPRO, VAL, VPA, VFL, VPRO. Results showed that OBPIs possessed higher protein contents (80%) and higher molecular weight (MW > 20kDa) than the OBPH with an average of 45% protein content and MW < 15kDa. OBPIs exhibited higher levels of aromatic amino acid residue (p < 0.05) compared to OBPHs. Meanwhile, aromatic amino acid contents varied among OBPHs due to difference in specificities of proteases. In the peroxyl radical scavenging (ORAC) assay, OBPHs from viscozyme treated brans performed better than those from cellulase. VFL and VAL had the highest ORAC value ranging from 565 ± 80 -640 ± 57 µM TE/g respectively compared to 406.3 ± 43 -506.7 ± 65 µM TE/g for hydrolysates from cellulase treated bran. VISC and iv CELL isolates had lower ORAC values ranging from 107.9 ± 13.5 -305.8 ± 28.3 µM TE/g respectively compared to OBPHs. Hydrolysis increased peroxyl radical scavenging activities (ORAC) but lowered hydroxyl radical (HO•) potentials while only minor changes were observed in the superoxide anion radical scavenging power. OBPIs and OBPHs had no effect on lipid peroxides in non-stored samples (NS) and -20 0 C storage, however storage at 4 0 C, cellulase treated OBPHs had lower concentration of lipid peroxide compared to viscozyme.Data on the effect of OBPI and OBPH on arsenic showed that at -20 0 C, treated samples had higher concentration of As (III) compared to NS, but mostly lower concentration compared to 4 0 C.Concentration of As (V) in treated samples is mostly lower compared to NS and 4 0 C. Non-stored samples (NS) treated with CELL and CFL had the highest (p < 0.05) c...

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Identification of peptides, metal binding and lipid peroxidation activities of HPLC fractions of hydrolyzed oat bran proteins

Cited by 38 publications

References 28 publications

Physicochemical, antioxidant, calcium binding, and angiotensin converting enzyme inhibitory properties of hydrolyzed tomato seed proteins

Physicochemical, antioxidant, calcium binding, and angiotensin converting enzyme inhibitory properties of hydrolyzed tomato seed proteins

The chelating peptide (GPAGPHGPPG) derived from Alaska pollock skin enhances calcium, zinc and iron transport in Caco‐2 cells

Effects of Hydrolyzed Oat Bran Proteins on Free Radicals, Toxic Metals and Lipid Oxidation

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