Identification of peptides with antioxidant, anti-lipoxygenase, anti-xanthine oxidase and anti-tyrosinase activities from velvet antler blood

Ding, Chuanbo; Hao, Mingqian; Ma, Shaoping; Zhang, Yiwen; Yang, Jiali; Ding, Qiteng; Sun, Shuwen; Zhang, Jinping; Zhang, Ying; Liu, Wencong

doi:10.1016/j.lwt.2022.113889

Cited by 17 publications

(37 citation statements)

References 52 publications

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“…Interestingly, TIP1, TIP2, RF1, and KNN1 were listed as the top three antioxidant peptide candidates of both properties. There were several reports on the correlation between antioxidant and anti-tyrosinase properties as the high anti-oxidative peptides also showed strong anti-tyrosinase activity [ 23 , 55 , 56 , 57 ]. Therefore, the reduction in melanin content of melanoma cells after treatment with TIP2 and RF1 could be the combined effects from antioxidative, anti-tyrosinase, and ROS reduction mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Computer-Aided Virtual Screening and In Vitro Validation of Biomimetic Tyrosinase Inhibitory Peptides from Abalone Peptidome

Kongsompong

E-kobon

Taengphan

et al. 2023

IJMS

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Hyperpigmentation is a medical and cosmetic problem caused by an excess accumulation of melanin or the overexpression of the enzyme tyrosinase, leading to several skin disorders, i.e., freckles, melasma, and skin cancer. Tyrosinase is a key enzyme in melanogenesis and thus a target for reducing melanin production. Although abalone is a good source of bioactive peptides that have been used for several properties including depigmentation, the available information on the anti-tyrosinase property of abalone peptides remains insufficient. This study investigated the anti-tyrosinase properties of Haliotis diversicolor tyrosinase inhibitory peptides (hdTIPs) based on mushroom tyrosinase, cellular tyrosinase, and melanin content assays. The binding conformation between peptides and tyrosinase was also examined by molecular docking and dynamics study. KNN1 showed a high potent inhibitory effect on mushroom tyrosinase with an IC50 of 70.83 μM. Moreover, our selected hdTIPs could inhibit melanin production through the reductions in tyrosinase activity and reactive oxygen species (ROS) levels by enhancing the antioxidative enzymes. RF1 showed the highest activity on both cellular tyrosinase inhibition and ROS reduction. leading to the lower melanin content in B16F10 murine melanoma cells. Accordingly, it can be assumed that our selected peptides exhibited high potential in medical cosmetology applications.

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Section: Discussionmentioning

confidence: 99%

Computer-Aided Virtual Screening and In Vitro Validation of Biomimetic Tyrosinase Inhibitory Peptides from Abalone Peptidome

Kongsompong

E-kobon

Taengphan

et al. 2023

IJMS

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“…A lot of research have been conducted on the bioactive peptides in chicken blood (Li et al., 2019; Nikhita & Sachindra, 2021; Zheng et al., 2018), among which, antioxidant peptides have garnered attention as a potent and readily available natural antioxidant in the body. These peptides with amino acid sequences ranging from 2 to 20 residues (Admassu et al., 2018; Hou et al., 2019; Li et al., 2021) could scavenge free radicals and thus aid in the prevention of cardiovascular diseases (Aguilar‐Toalá & Liceaga, 2021; Chen et al., 2021), as excessive production and accumulation of free radicals may lead to protein deformation, DNA mutation, and membrane phospholipid oxidation (Ding et al., 2022). Additionally, it exerts protective effects by modulating the expression of pro‐ and antiapoptotic proteins via antioxidant enzymes (Wong et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Purification, characterization, and in vitro digestion of novel antioxidant peptides from chicken blood hemoglobin

Wang,

Mao,

Zhang

et al. 2024

Journal of Food Science

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The study aimed to purify and characterize antioxidant peptides from chicken blood hemoglobin hydrolysate. The fraction M2 (< 3 KDa) with the strongest antioxidant activity was isolated by ultrafiltration, and its DPPH (1,1‐diphenyl‐2‐picryl‐hydrazyl radical) free radical scavenging rate, ABTS [2,2′‐Azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate)] free radical scavenging rate, and iron ion chelation activity were 82.91%, 77.49%, and 80.99%, respectively. After in vitro digestion, the antioxidant capacity of chicken blood hydrolysate was significantly higher than that before digestion (p < 0.05). M2 exhibited the strongest antioxidant activity after stomach digestion, with a DPPH radical scavenging rate and iron ion chelating power of 82.91% and 79.61%, respectively. Component A was purified from M2 by Sephadex G‐25 gel chromatography. The peptide sequences were identified by LC‐MS/MS from fraction A, and four peptides, AEDKKLIQ (944.54 Da), APAPAAK (625.36 Da), LSDLHAHKL (1033.57 Da), and LSNLHAYNL (1044.54 Da) were synthesized using the solid‐phase peptide method, among which APAPAAK was a novel antioxidant peptide. Molecular docking was used to simulate the binding of these four peptides to the key active site of Keap1 via hydrogen bonding. This study suggests that chicken blood may provide a new natural source of antioxidant peptides.

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“…2,3 To date, Velvet antlers derived from sika deer and red deer have been designated as the authentic medicinal varieties in the world, especially in China, Japan, and South Korea. 3,4 Nevertheless, due to the diversity of deer species, high medicinal value, and remarkable price differences among different varieties, there is an endless stream of fake Velvet antlers in the market. To pursue a pecuniary advantage, some unscrupulous businessmen have seriously disrupted the market of TMMs by passing shoddy goods off as goods and passing the adulterates for the true commodities in the sale of Velvet antler.…”

Section: Introductionmentioning

confidence: 99%

“…As a valuable animal-derived traditional medicinal material (TMM), Velvet antler is officially documented in the Chinese Pharmacopoeia and derived from the nonossified and densely hairy young horns of male sika deer or red deer . It has been widely used for over 2000 years with extensive pharmacological activities and functions for medical care, including the regulation of the immune system, blood system, bone metabolism, and glucose metabolism, as well as anticancer, anti-inflammatory, antioxidant, antifatigue, antibacterial, antivirus, antistress, and analgesic properties. , To date, Velvet antlers derived from sika deer and red deer have been designated as the authentic medicinal varieties in the world, especially in China, Japan, and South Korea. , …”

Section: Introductionmentioning

confidence: 99%

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Identification and Quality Evaluation of Velvet Antler by DNA Barcoding and Stable Isotope Techniques Combined with Chemometrics

Zeng

Zhou

et al. 2022

ACS Omega

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This study aimed to identify Velvet antler and its counterfeits and to further evaluate their quality. Mitochondrial cytochrome b (Cytb) was used as a target gene to identify Velvet antler samples, and a DNA barcoding method was established for species origin identification in Velvet antlers. After identification, the stable isotope contents and ratios were adopted to evaluate the quality of different specifications of authentic Velvet antler in combination with chemometrics. Two stable isotope contents (C % and N %) and ratios (δ13C and δ15N) in three kinds of Velvet antler slices of different specifications, namely, wax slices, powder slices, and bone slices, were determined. Nine Velvet antler samples sold in the market were identified for label conformity. Only two samples were consistent with the labeled species, and the others were counterfeits. The three slices of Velvet antler of different specifications were clearly distinguished by principal component analysis and hierarchical cluster analysis. Then, the discriminant model of partial least squares discriminant analysis was established, and 100% discrimination accuracy was observed in this model. All the Velvet antler slice samples of different specification samples were grouped clearly according to their sources. In summary, it is feasible for the identification and quality grade evaluation of Velvet antler by DNA barcoding based on mitochondrial Cytb and stable isotope techniques combined with chemometric analysis. The establishment of this method also provided a reference for the evaluation of other animal-derived medicinal materials.

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Identification of peptides with antioxidant, anti-lipoxygenase, anti-xanthine oxidase and anti-tyrosinase activities from velvet antler blood

Cited by 17 publications

References 52 publications

Computer-Aided Virtual Screening and In Vitro Validation of Biomimetic Tyrosinase Inhibitory Peptides from Abalone Peptidome

Computer-Aided Virtual Screening and In Vitro Validation of Biomimetic Tyrosinase Inhibitory Peptides from Abalone Peptidome

Purification, characterization, and in vitro digestion of novel antioxidant peptides from chicken blood hemoglobin

Identification and Quality Evaluation of Velvet Antler by DNA Barcoding and Stable Isotope Techniques Combined with Chemometrics

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