Identification of PER-1 extended-spectrum β-lactamase producingPseudomonas aeruginosaclinical isolates of the international clonal complex CC11 from Hungary and Serbia

Abstract

PER-1 extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa clinical isolates from Budapest, Hungary, and Belgrade, Serbia, were characterized by molecular methods. Two PER-1-positive isolates were recovered from sporadic cases in Budapest and a small cluster of PER-1-positive infections involving four patients were identified at a Belgrade hospital. A class 1 integron harbouring a bla(OXA-2)beta-lactamase gene and four other gene cassettes was detected in both the Budapest and the Belgrade isolates. The two P. aeruginosa isolates from Budapest also carried another class 1 integron containing bla(OXA-74), aac(6')-Ib-cr and cmlA7 genes in its variable region. The aac(6')-Ib-cr fluoroquinolone-acetylating aminoglycoside acetyltransferase gene is described here for the first time in P. aeruginosa. Multilocus sequence typing (MLST) revealed that the PER-1 positive P. aeruginosa isolates identified in this study display ST235, a sequence type that belongs to clonal complex CC11. Two bla(PER-1)-positive P. aeruginosa reference isolates from France and Belgium could also be assigned to complex CC11 by MLST. Our results underscore the role of complex CC11 in the dissemination of bla(PER-1) among P. aeruginosa clinical isolates.

Most Multidrug-Resistant Pseudomonas aeruginosa Isolates from Hospitals in Eastern France Belong to a Few Clonal Types

“…ST235 was the founder ST of the clonal complex CC235 (previously described as CC11/BG11). CC235 has been long known as responsible for outbreaks throughout Europe, Asia, and South America (8,11,15,17,18,20,27) while armed with various ESBLs (such as PER-1, GES-1, and BEL-1) as well as MBLs (such as VIM-1, VIM-4, and IMP-1) (Fig. 2).…”

Molecular Characterization of bla NDM-1 in a Sequence Type 235 Pseudomonas aeruginosa Isolate from France

“…The ability of P. aeruginosa to accumulate overproduction of intrinsic resistance mechanisms, as well as the combination of various genetic determinants, including nonplasmid lateral exchange of resistance regions (21), results in multidrug-resistant or extensively drug-resistant isolates. Multilocus sequence typing, conducted according to published protocols (28; http://pubmlst.org/paeruginosa/), assigned HIABP11 to sequence type 235 (ST235), which has been encountered in clinical and environmental isolates of different countries (29,30), including Serbia (31). ST235 is the primary founder of the successful epidemic clonal complex 235 (CC235), associated with various ␤-lactamase genes, including PER-1 extended-spectrum ␤-lactamase and VIM-type metallo-␤-lactamases (32,33).…”

Prevalence of blaPER-1 and Integrons in Ceftazidime-Resistant Gram-Negative Bacteria at a University Hospital in Turkey

“…The rate of bla PER-1 -positive isolates estimated at our hospital is mostly due to clonally related strains, and it is lower than the rates observed at other hospitals in our country (1,13,14). Studies conducted in other countries have reported lower rates of bla PER-1 -positive isolates compared to Turkey; however, these studies also showed that these percentages tend to increase when specific selection criteria are applied (15,16). In the present study, our results showed a nearly homogenous distribution of the bla PER-1 -positive P. aeruginosa isolates across the hospital wards; on the other hand, most of the bla PER-1 -positive A. baumannii complex bacteria were isolated from the emergency intensive care unit.…”