Identification of Periodontal Bacteria Using DNA Probes Based on Bacterial 16S Ribosomal RNA Gene. Colony Hybridization Method.

Sawada, Satoko; Kokeguchi, Susumu; Miyamoto, Manabu; Sawada, Kayo; Fujimoto, C.; Wataki, Tetsuji; Hirosue, Masaru; Shimizu, Akifumi; Chou, Hsin-Hua; Kurihara, Hidemi; Arai, Hideo; Takashiba, Shogo; Murayama, Yoji

doi:10.2329/perio.40.4_475

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2000

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“…Patients were assigned as 'P. gingivalis-infected' by having at least 2 subgingival plaque samples positive for P. gingivalis determined by the polymerase chain reaction method as described previously (Sawada et al 1998). The periodontitis patients were clinically diagnosed as having EOP (16 patients, mean age 26 years old ranging 18 to 35, 9 male and 7 female) and AP (20 patients, mean age 52 years old ranging 34 to 69, 5 male and 15 female) from the criteria as described previously (Takahashi et al 1997a).…”

Section: Methodsmentioning

confidence: 99%