Identification of person and quantification of human DNA recovered from mosquitoes (Culicidae)

Ćurić, Goran; Hercog, Rajna; Vrselja, Zvonimir; Wagner, Jasenka

doi:10.1016/j.fsigen.2013.07.011

Cited by 33 publications

(44 citation statements)

References 17 publications

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“…Mosquito 3, which had a mixture of genetic profiles, had 0.058803 ng/µL human blood and fed on two separate women. Curic et al (2014) described the possibility to quantify human DNA in all mosquitoes analyzed, which is an important factor when analyzing mosquitoes as forensic evidence. Genetic analysis revealed that it is possible to identify individuals at a crime scene based on the amount of DNA that can be found in mosquitoes collected from a crime scene, which can help save time and resources.…”

Section: Resultsmentioning

confidence: 99%

“…Mosquitoes (Culicidae) are a widely distributed source of human genetic material that can easily be found at crime scenes (Spitarelli, 2006). Genetic profiles obtained from human blood found in the digestive tracts of mosquitoes, for example, can assist in the investigation of crimes committed in a closed environment (such as a room or vehicle; Spitarelli, 2006;Curic, 2014). Comparison of the samples to the suspect's with a DNA database of criminals may also indicate whether a crime is a repeated offense (Wallace et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…This characteristic is an additional advantage because it can result in more than one human DNA profile (Clayton et al, 1998;Gill et al, 1998;González-Andrade et al, 2006) by female mosquitoes that may have fed on the victim or criminal. Therefore, the links between the suspect, victim, crime, and/or crime scene can be achieved by analyzing mosquitoes (Curic et al, 2014). In this study, we compared the profile of human DNA saliva with blood from the digestive tract of mosquitoes found in the same environment.…”

Section: Introductionmentioning

confidence: 99%

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Trace samples of human blood in mosquitoes as a forensic investigation tool

et al. 2015

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Investigations of any type of crime invariably starts at the crime scene by collecting evidence. Thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. Hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for genetic analysis as reference samples. We examined the allele frequencies of 15 short tandem repeat loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) and amelogenin. A total of 26 female hematophagous mosquitoes were identified as Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus; we were able to obtain 11 forensically valid genetic profiles, with a minimum of 0.028203 ng/μL of human DNA. Thus, the results of this study showed that it was possible to correlate human genetic information from mosquitoes with the volunteer reference samples, which validates the use of this information as forensic evidence. Furthermore, we observed mixed genetic profiles from one mosquito. Therefore, it is clearly important to collect these insects indoors where crimes were committed, because it may be possible to find intact genetic profiles of suspects in the blood found in the digestive tract of hematophagous mosquitoes for later comparison to identify an offender and/or exclude suspects

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Trace samples of human blood in mosquitoes as a forensic investigation tool

et al. 2015

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“…Badania przeprowadzone na komarach przez Curic i wsp. wykazały, że ustalenie ludzkiego profilu 15 loci STR jest możliwe nawet do 88 godzin po uką szeniu [42]. Sam bierny kontakt owadów synantro pijnych z kurzem, np.…”

Section: Isolation and Analysis Of Human Dna From Various Types Of Enunclassified

“…haematophagous (bloodfeeding) insects: mosquitoes [29,[33][34][35][36][37], lice [28,[38][39][40] and bed bugs [41]. Studies conducted on mosquitoes by Cu ric et al have established that it is possible to de termine the human profile of 15 STR loci for up to 88 hours after biting [42]. The passive contact of synanthropic insects with dust alone, for example in an apartment, is sufficient for the recovery of the socalled environmental human DNA which can be used in forensic genetic analysis to investigate the activity of specific people in a given location [43].…”

Section: Wykorzystanie śLadów Entomologicznych Jako źRódła Ludzkiego mentioning

confidence: 99%

Insects feeding on cadavers as an alternative source of human genetic material

Skowronek¹,

Tomsia²,

Droździok³

et al. 2014

amsik

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StreszczenieW niektórych sprawach kryminalnych wykorzystanie klasycznych źródeł ludzkiego materiału genetycznego jest utrud nione lub nawet niemożliwe. Rozwiązaniem może być wykorzystanie owadów, zwłaszcza larw muchówek, żerujących na zwłokach. Aktualny przegląd opisów przypadków oraz badań eksperymentalnych dostępnych w bazach biomedycz nych wykazał, że owady mogą stanowić cenne źródło ludzkiego kwasu dezoksyrybonukleinowego (DNA), zarówno mitochondrialnego, jak i genomowego, umożliwiającego efektywną analizę, odpowiednio sekwencji regionów hiper zmiennych (HVR) i profili krótkich powtórzeń tandemowych (STR). Optymalnym źródłem ludzkiego DNA jest wole (część jelita) aktywnych larw muchówek III stadium. Puparia i wydaliny owadów również mogą być wykorzystane w praktyce genetycznosądowej zamiast zawartości przewodu pokarmowego.Słowa kluczowe: entomologia sądowa, genetyka sądowa, larwy muchówek, identyfikacja osobnicza. AbstractIn some criminal cases, the use of classical sources of human genetic material is difficult or even impossible. One so lution may be the use of insects, especially blowfly larvae which feed on corpses. A recent review of case reports and experimental studies available in biomedical databases has shown that insects can be a valuable source of human mito chondrial and genomic deoxyribonucleic acid (DNA), allowing for an effective analysis of hypervariable region (HVR) sequences and short tandem repeat (STR) profiles, respectively. The optimal source of human DNA is the crop (a part of the gut) of active thirdinstar blowfly larvae. Pupae and insect faeces can be also used in forensic genetic practice instead of the contents of the alimentary tract.Key words: forensic entomology, forensic genetics, blowfly larvae, personal identification. WprowadzenieEntomologia sądowa stopniowo zyskuje coraz większe uznanie wśród przedstawicieli organów ści

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Insects as vectors of DNA in a forensic context

Durdle

2019

WIREs Forensic Science

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Insects have historically been used in criminal investigations to provide information in relation to postmortem intervals (PMIs) but the field of forensic entomology is expanding. It is now recognized that insects can act as vectors of human and mammalian DNA through the consumption of biological material, with extraneous DNA able to be extracted and genotyped from all stages of the life cycle, and insect feces and regurgitant (artifacts). To date, DNA recovered from insects have been used to inform investigations into neglect and homicide, link body parts, and to identify victims of crime. It may also potentially be used to identify assailants, confirm the food source of insects to determine their relevance in PMI calculations, determine if a crime has occurred, identify scenes of crimes, and link people to locations and other individuals. However, insects which have consumed biological material may also transfer DNA by traveling to new areas, or depositing it via their artifacts, either within crime scenes or laboratories, or at locations distant to the food source. This could contaminate forensic evidence, confound investigations, and/or falsely incriminate or exclude an individual. Therefore, it is important that there is increased awareness into both the utility of insects as vectors of forensically relevant DNA, and the potential for contamination. This article is categorized under: Forensic Biology > Forensic DNA Technologies Forensic Science in Action/Crime Scene Investigation > Crime Scene Examination Forensic Biology > Interpretation of Biological Evidence Forensic Biology > Body Fluid Identification

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Identification of person and quantification of human DNA recovered from mosquitoes (Culicidae)

Cited by 33 publications

References 17 publications

Trace samples of human blood in mosquitoes as a forensic investigation tool

Trace samples of human blood in mosquitoes as a forensic investigation tool

Insects feeding on cadavers as an alternative source of human genetic material

Insects as vectors of DNA in a forensic context

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