Identification of phenolic markers for saffron authenticity and origin: An untargeted metabolomics approach

Senizza, Biancamaria; Rocchetti, Gabriele; Ghisoni, Silvia; Busconi, Matteo; Pascual, Marcelino de los Mozos; Fernández, José Antonio; Lucini, Luigi; Trevisan, Marco

doi:10.1016/j.foodres.2019.108584

Cited by 59 publications

(28 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, Soxhlet extraction promoted the highest recovery of flavones (2.52 mg/g). Colchicum flowers are reported to be very similar from a morphological point of view to those of Crocus Sativus L. Overall, both plant species can be considered as a good source of (poly)-phenolic compounds In this regard, the saffron flower has been described as rich in flavonoids (such as flavonols and flavones), hydroxycinnamic acids, and lignans [10,17,18]. Regarding saffron alkaloids, Amin Mir and co-authors [19] found this class of compounds in both water and methanolic extracts of flowers.…”

Section: Phytochemical Profiling Of the Different Extractsmentioning

confidence: 98%

“…The untargeted phytochemical profile of the different C. triphyllum extracts was investigated through ultra-high-pressure liquid chromatography (Agilent 1290 HPLC liquid chromatograph; Agilent Technologies, Santa Clara, CA, USA) coupled to a quadrupole-time-of-flight mass spectrometer (Agilent 6550 iFunnel; Agilent Technologies, Santa Clara, CA, USA). The experimental conditions for the analysis of plant extracts using untargeted metabolomics were optimized in previous works from our research group [9][10][11]. The mass spectrometer acquired ions in the range 50-1200 m/z in positive (ESI+) scan mode.…”

Section: Uhplc-qtof Profiling Of Polyphenols and Alkaloidsmentioning

confidence: 99%

See 1 more Smart Citation

Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron)

Senizza

Rocchetti

Okur

et al. 2020

Foods

Self Cite

View full text Add to dashboard Cite

In this work, the phytochemical profile and the biological properties of Colchicum triphyllum (an unexplored Turkish cultivar belonging to Colchicaceae) have been comprehensively investigated for the first time. Herein, we focused on the evaluation of the in vitro antioxidant and enzyme inhibitory effects of flower, tuber, and leaf extracts, obtained using different extraction methods, namely maceration (both aqueous and methanolic), infusion, and Soxhlet. Besides, the complete phenolic and alkaloid untargeted metabolomic profiling of the different extracts was investigated. In this regard, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) allowed us to putatively annotate 285 compounds when considering the different matrix extracts, including mainly alkaloids, flavonoids, lignans, phenolic acids, and tyrosol equivalents. The most abundant polyphenols were flavonoids (119 compounds), while colchicine, demecolcine, and lumicolchicine isomers were some of the most widespread alkaloids in each extract analyzed. In addition, our findings showed that C. triphyllum tuber extracts were a superior source of both total alkaloids and total polyphenols, being on average 2.89 and 10.41 mg/g, respectively. Multivariate statistics following metabolomics allowed for the detection of those compounds most affected by the different extraction methods. Overall, C. triphyllum leaf extracts showed a strong in vitro antioxidant capacity, in terms of cupric reducing antioxidant power (CUPRAC; on average 96.45 mg Trolox Equivalents (TE)/g) and ferric reducing antioxidant power (FRAP) reducing power (on average 66.86 mg TE/g). Interestingly, each C. triphyllum methanolic extract analyzed (i.e., from tuber, leaf, and flower) was active against the tyrosinase in terms of inhibition, recording the higher values for methanolic macerated leaves (i.e., 125.78 mg kojic acid equivalent (KAE)/g). On the other hand, moderate inhibitory activities were observed against AChE and α-amylase. Strong correlations (p < 0.01) were also observed between the phytochemical profiles and the biological activities determined. Therefore, our findings highlighted, for the first time, the potential of C. triphhyllum extracts in food and pharmaceutical applications.

show abstract

Section: Phytochemical Profiling Of the Different Extractsmentioning

confidence: 98%

Section: Uhplc-qtof Profiling Of Polyphenols and Alkaloidsmentioning

confidence: 99%

Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron)

Senizza

Rocchetti

Okur

et al. 2020

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

“…Compared to classical PLS-DA, OPLS-DA and SPLS-DA are currently used comparatively little in the data analysis of metabolomics experiments, but this is expected to change over time. For example, the authenticity of saffron [ 173 ], Grana Padano cheese [ 174 ] and organically grown rice [ 175 ] were recently verified using OPLS-DA. However, no more than two sample groups were analyzed in these studies, as this is the strength of the method as described above.…”

Section: From Non-targeted Data Sets To Marker Compoundsmentioning

confidence: 99%

Food Phenotyping: Recording and Processing of Non-Targeted Liquid Chromatography Mass Spectrometry Data for Verifying Food Authenticity

Creydt

Fischer

2020

Molecules

View full text Add to dashboard Cite

Experiments based on metabolomics represent powerful approaches to the experimental verification of the integrity of food. In particular, high-resolution non-targeted analyses, which are carried out by means of liquid chromatography-mass spectrometry systems (LC-MS), offer a variety of options. However, an enormous amount of data is recorded, which must be processed in a correspondingly complex manner. The evaluation of LC-MS based non-targeted data is not entirely trivial and a wide variety of strategies have been developed that can be used in this regard. In this paper, an overview of the mandatory steps regarding data acquisition is given first, followed by a presentation of the required preprocessing steps for data evaluation. Then some multivariate analysis methods are discussed, which have proven to be particularly suitable in this context in recent years. The publication closes with information on the identification of marker compounds.

show abstract

“…OPLS-DA, a supervised latent structures-discriminant analysis method, utilizes class information to maximize the separation between classes and to minimize the separation between intra-groups. In order to further characterize the differences in secondary metabolites among the xylem and phloem, OPLS-DA was performed to obtain better discrimination among the two sections [17,22]. The scores plot of OPLS-DA demonstrated that all samples were clearly classified into two groups: xylem and phloem.…”

Section: Opls-da Of Samplesmentioning

confidence: 99%

“…The standards of procyanidin B2 (5), daidzin (7), dihydroquercetin (8), genistin (9), liquiritigenin (12), calycosin (14), naringenin (15), genistein (16), medicarpin (20), prunetin (21) and biochanin A (22) were purchased from Nanjing Liangwei biotechnology Co., Ltd. (Nanjing, China). Epicatechin (6), rutin (10), kaempferol (17) and formononetin (19) were purchased from the Chinese National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

et al. 2019

View full text Add to dashboard Cite

Spatholobi Caulis (SC), the vine stem of Spatholobus suberectus Dunn, is a widely used traditional Chinese medicine (TCM) for the treatment of blood stasis syndrome and related diseases. Xylem and phloem are the main structures of SC and the color of xylem in SC is red brown or brown while the phloem with resin secretions is reddish brown to dark brown. They are alternately arranged in a plurality of concentric or eccentric rings. In order to investigate the distribution patterns of metabolites in xylem and phloem of SC, an analytical method based on UFLC–QTRAP–MS/MS was established for simultaneous determination of 22 constituents including four flavanols, nine isoflavones, two flavonols, two dihydroflavones, one flavanonol, one chalcone, one pterocarpan, one anthocyanidin and one phenolic acid in the samples (xylem and phloem) from Laos. Furthermore, according to the contents of 22 constituents, heat map, principal components analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS–DA) and t–test were used to evaluate the samples and discover the differences between xylem and phloem of SC. The results indicated that the measured ingredients in xylem and phloem were significantly different. To be specific, the contents of flavonoids in xylem were higher than that in phloem, while the content of protocatechuic acid showed a contrary tendency. This study will not only reveal the distribution patterns of metabolites in xylem and phloem of SC but also facilitate further study on their quality formation.

show abstract

Identification of phenolic markers for saffron authenticity and origin: An untargeted metabolomics approach

Cited by 59 publications

References 25 publications

Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron)

Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron)

Food Phenotyping: Recording and Processing of Non-Targeted Liquid Chromatography Mass Spectrometry Data for Verifying Food Authenticity

A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

Contact Info

Product

Resources

About