Identification of phenolic metabolites in human urine after the intake of a functional food made from grape extract by a high resolution LTQ-Orbitrap-MS approach

Sasot, Gemma; Martı́nez-Huélamo, Miriam; Vallverdú‐Queralt, Anna; Mercader-Martí, Mercè; Estruch, Ramón; Lamuela-Raventós, Rosa M.

doi:10.1016/j.foodres.2017.01.020

Cited by 52 publications

(36 citation statements)

References 55 publications

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“…Compound 25 (RT = 19.319 min) was tentatively identified as m-coumaric acid with the precursor [M − H] − m/z at 163.0406 and confirmed by the MS/MS spectra (Figure 2), which exhibited the fragments at m/z 119 due to the loss of CO 2 [54]. Compound 47 (dihydroferulic acid 4-O-glucuronide, m/z at 371.0986) and compound 49 (dihydrocaffeic acid 3-O-glucuronide, m/z at 357.0811) were both detected only in the negative ionization mode, and the characteristic loss of the glucuronide (176 Da) moiety was observed in both compounds, which produced the fragment ions at m/z 195 and at m/z 181, respectively [55].…”

Section: Phenolic Acidsmentioning

confidence: 99%

Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels

Suleria

Barrow

Dunshea

2020

Foods

222

157

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Fruit peels have a diverse range of phytochemicals including carotenoids, vitamins, dietary fibres, and phenolic compounds, some with remarkable antioxidant properties. Nevertheless, the comprehensive screening and characterization of the complex array of phenolic compounds in different fruit peels is limited. This study aimed to determine the polyphenol content and their antioxidant potential in twenty different fruit peel samples in an ethanolic extraction, including their comprehensive characterization and quantification using the LC-MS/MS and HPLC. The obtained results showed that the mango peel exhibited the highest phenolic content for TPC (27.51 ± 0.63 mg GAE/g) and TFC (1.75 ± 0.08 mg QE/g), while the TTC (9.01 ± 0.20 mg CE/g) was slightly higher in the avocado peel than mango peel (8.99 ± 0.13 mg CE/g). In terms of antioxidant potential, the grapefruit peel had the highest radical scavenging capacities for the DPPH (9.17 ± 0.19 mg AAE/g), ABTS (10.79 ± 0.56 mg AAE/g), ferric reducing capacity in FRAP (9.22 ± 0.25 mg AA/g), and total antioxidant capacity, TAC (8.77 ± 0.34 mg AAE/g) compared to other fruit peel samples. The application of LC-ESI-QTOF-MS/MS tentatively identified and characterized a total of 176 phenolics, including phenolic acids (49), flavonoids (86), lignans (11), stilbene (5) and other polyphenols (25) in all twenty peel samples. From HPLC-PDA quantification, the mango peel sample showed significantly higher phenolic content, particularly for phenolic acids (gallic acid, 14.5 ± 0.4 mg/g) and flavonoids (quercetin, 11.9 ± 0.4 mg/g), as compared to other fruit peel samples. These results highlight the importance of fruit peels as a potential source of polyphenols. This study provides supportive information for the utilization of different phenolic rich fruit peels as ingredients in food, feed, and nutraceutical products.

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Section: Phenolic Acidsmentioning

confidence: 99%

Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels

Suleria

Barrow

Dunshea

2020

Foods

222

157

View full text Add to dashboard Cite

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“…The MS 2 of m/z 449 showed a characteristic product ion at m/z 287 (eriodictyol) generated by the loss of a hexose moiety (−162 Da). Additionally, eriodictyol-O-hexoside was tentatively identified by comparison with the mass spectra of previous studies using the LTQ-Orbitrap to analyze red wine [31] and grape pomace [47]. To the best of our knowledge, this is the first report of eriodictyol-O-hexoside in raisins of these varieties, although it has been previously identified in grape skins [48].…”

Section: Flavanones and Derivativesmentioning

confidence: 73%

“…Supplementary Materials: The following are available online at http://www.mdpi.com/1420-3049/25/6/1281/s1, Table S1: Phenolic compounds tentatively identified using LC-ESI-LTQ-Orbitrap-MS in negative mode: Retention time (min), error (ppm), and reference used for identification [21,33,34,43,47,[55][56][57][58][59][60][61].…”

Section: Discussionmentioning

confidence: 99%

A Targeted Approach by High Resolution Mass Spectrometry to Reveal New Compounds in Raisins

et al. 2020

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Raisins are dried grapes mostly obtained from cultivars of Vitis vinifera L. and are extensively consumed worldwide. They are rich in bioactive compounds such as polyphenols, which are associated with a broad range of health benefits. The aim of the present study was to compare the phenolic profiles of three different raisin varieties (Thompson seedless, Muscat, and sultanas). Total polyphenols (TPs) were evaluated by the Folin–Ciocalteu (F–C) assay and significant differences were observed among all raisin varieties. Furthermore, liquid chromatography coupled with electrospray ionization hybrid linear ion trap quadrupole-Orbitrap-mass spectrometry (LC/ESI-LTQ-Orbitrap-MS) was employed for the comprehensive identification of phenolic constituents. A total of 45 compounds were identified, including hydroxybenzoic and hydroxycinnamic acids, flavanoids, flavonoids, flavonols, flavones, and stilbenoids. The three varieties of raisins showed a similar phenolic profile, although the highest number of phenolic compounds was identified in Muscat raisins owing to the proanthocyanidins extracted from their seeds, while stilbenoids were not detected in the Thompson variety.

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“…From the 880 unique citations screened, 32 original papers fulfilled the inclusion criteria of this review (Fig. 1 ) [ 6 , 15 , 19 , 20 , 23 , 24 , 29 , 33 , 55 , 66 , 69 , 71 , 79 , 84 , 85 , 96 , 97 , 99 , 107 , 117 , 118 , 121 , 122 , 131 , 138 , 142 , 143 , 149 , 150 , 157 , 158 , 168 , 171 , 177 ]. The grape extract was included in this review, considering that elimination of water fraction during desiccation does not drastically change the overall polyphenol content.…”

Section: Resultsmentioning

confidence: 99%

“…Except for tartaric acid and resveratrol, no other promising candidate BFI were retained for grapes. Many other studies with a targeted approach focused on the non-specific major polyphenols of grape, namely anthocyanins [ 15 , 55 , 157 ], catechins, and procyanidins [ 99 , 149 , 150 , 157 ]. Other non-specific small phenolics, including a range of hydroxybenzoic acids (gallic acid, dihydroxybenzoic acid, vanillic acid, hydroxyhippuric acid, etc.…”

Section: Discussionmentioning

confidence: 99%

Food intake biomarkers for berries and grapes

et al. 2020

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Grapes and berries are two types of widely consumed fruits characterized by a high content in different phytochemicals. However, their accurate dietary assessment is particularly arduous, because of the already wide recognized bias associated with self-reporting methods, combined with the large range of species and cultivars and the fact that these fruits are popularly consumed not only in fresh and frozen forms but also as processed and derived products, including dried and canned fruits, beverages, jams, and jellies. Reporting precise type and/or quantity of grape and berries in FFQ or diaries can obviously be affected by errors. Recently, biomarkers of food intake (BFIs) rose as a promising tool to provide accurate information indicating consumption of certain food items. Protocols for performing systematic reviews in this field, as well as for assessing the validity of candidate BFIs have been developed within the Food Biomarker Alliance (FoodBAll) Project. This paper aims to evaluate the putative BIFs for blueberries, strawberries, raspberries, blackberries, cranberries, blackcurrant, and grapes. Candidate BFIs for grapes were resveratrol metabolites and tartaric acid. The metabolites considered as putative BFI for berries consumption were mostly anthocyanins derivatives together with several metabolites of ellagitannins and some aroma compounds. However, identification of BFIs for single berry types encountered more difficulties. In the absence of highly specific metabolites reported to date, we suggested some multi-metabolite panels that may be further investigated as putative biomarkers for some berry fruits.

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Identification of phenolic metabolites in human urine after the intake of a functional food made from grape extract by a high resolution LTQ-Orbitrap-MS approach

Cited by 52 publications

References 55 publications

Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels

Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels

A Targeted Approach by High Resolution Mass Spectrometry to Reveal New Compounds in Raisins

Food intake biomarkers for berries and grapes

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