Identification of phenolics in litchi and evaluation of anticancer cell proliferation activity and intracellular antioxidant activity

Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology with Maximal Phenolic Yield and Antioxidant Activity from <em>Acer truncatum</em> Leaves

“…Compound 6 (11.05 min) with a molecular ion at m/z 285.0612 was identified as uralenneoside as it fragmented at m/z 153 due to the loss of hydroxybenzoic acid unit and at m/z 109 because of another loss of a CO2 group from the carboxylic acid moiety, and its MS/MS data were accordance with the fragmentation pattern reported by Yu et al [63]. Compound 12 (16.91 min), with deprotonated ion at m/z 863.1819, was identified as cinnamtannin B1 as it consisted of three epicatechin units, which was reflected by the daughter ion at m/z 289 [64].…”

Phytochemical profiles and cellular antioxidant activity of Malus doumeri (bois) chevalier on 2,2′-azobis (2-amidinopropane) dihydrochloride (ABAP)-induced oxidative stress

“…After treatment with oxidant ABAP (600 µM) for 1 h, cells were collected and treated with cell lysis buffer, containing 20 mM Tris (pH 7.5), 150 mM NaCl and 1% Triton X-100, at 4°C for 10 min. The intracellular activities of SOD, GSH-Px, and CAT of the lysed cells were further calculated, with the corresponding assay kits according to the manufacturer's instructions (Wen et al, 2015b), as well as the protein content. The final values were expressed as U/mg protein for both SOD and CAT activities, and mU/mg protein for GSH-Px activity.…”

“…Through a series of enzymatic reactions, intracellular antioxidant enzymes convert reactive radicals into less active radicals (Wen et al, 2015b). For example, SOD can catalyse the dismutation of highly active intracellular superoxide anion O2 − into O2 and hydrogen peroxide (H2O2), which is less active; CAT and GSH-Px can reverse the conversion of H2O2, which could be converted into more detrimental radicals, like hydroxyl radials (·OH), changing the H2O2 generated within cells to H2O and O2, thereby protecting cells from oxidative stress (Halliwell, 2012;Su et al, 2016).…”

Preparation of Prunella vulgaris polysaccharide-zinc complex and its antiproliferative activity in HepG2 cells

“…with modifications [19]. As described above, HepG2 cells were seeded in six-well microplates at a density of 4 × 10 5 cells/well.…”

“…The cell cycle distribution was measured by flow cytometry as previously described with some modification [19]. Briefly, HepG2 cells were seeded in six-well microplates at a density of 4 × 10 5 cells/well.…”