Identification of phenotypic and genotypic properties and cold adaptive mechanisms of novel freeze–thaw stress-resistant strain Pseudomonas mandelii from Antarctica

Mumcu, Hande; Cebeci, Emine Tuğçe Sarac; Kılıc, Meryem Menekse; Cebeci, Anil; Güneș, Yaǧmur; Karacan, Ilker; Öztug, Merve; Balcı, Nurgül; Karagüler, Nevin Gül

doi:10.1007/s00300-023-03114-y

Cited by 2 publications

(1 citation statement)

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“…Analyses were performed in Thermo Scientific Q Exactive HF-X mass spectrometer (MS) coupled with Thermo Scientific UltiMate 3000 RSLC Nano Ultra performance liquid chromatography (UPLC) as described previously ( Mumcu et al, 2023 ). Briefly, peptide separation involved reversed-phase liquid chromatography (RP-LC) using Acclaim PepMap C18 Trap Cartridge (5 μm, 100A, 300 μm i.d × 5 mm) and EASY-Spray ES902 RSLC C18 (2 μm, 100A 75 μm × 25 cm) columns.…”

Section: Methodsmentioning

confidence: 99%

Proteomic investigation of acute and chronic hypoxia /reoxygenation responsive proteins and pathways in H9C2 cardiomyoblasts

ÖZTUĞ,

KILINÇ,

ÖZTUĞ DURER

et al. 2024

Turkish Journal of Biology

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Background/aim Ischemic heart diseases continue to be a significant global cardiovascular problem in today’s world. Myocardial reperfusion (R) is provided with an effective and rapid treatment; however, it can lead to fatal results, as well as ischemia (I). This study aims to use proteomic analysis to assess proteins and pathways in H9C2 cardiomyoblast cells exposed to hypoxic conditions, followed by reoxygenation, representing I/R injury for both short and long terms, reflecting acute and chronic hypoxia, respectively. Utilizing advanced techniques, our goal is to identify and characterize key proteins undergoing alterations during these critical phases. Materials and methods H9C2 cardiomyoblasts, a commonly used cell line for simulating in vivo I/R damage, were exposed to normoxia and hypoxia (0.4% O 2 ) in six experimental groups: normoxia (3h), acute hypoxia (3h), acute hypoxia (3h) + reoxygenation (3h), normoxia (21h), chronic hypoxia (21h), and chronic hypoxia (21h) + reoxygenation (3h). Analyses were conducted using Nano LC/MSMS from tryptic digest of the whole cell lysates. Proteins were quantified using the label-free quantification (LFQ) algorithm in Proteome Discoverer 2.4. Results Proteomic analysis resulted in identification of 2383 protein groups. Proteins that differentially expressed in the various groups were identified (p < 0.05 among mean values for groups). Short-term hypoxia induces mitochondrial damage, energy demand, and cytoskeletal modifications. Chronic hypoxia triggers metabolic shifts, stress-response proteins, and extracellular matrix alterations. Data are available via ProteomeXchange with identifier PXD047994. Conclusion Our research provides in-depth insights into how H9C2 cardiomyoblasts respond to both short-term and prolonged oxygen deprivation. Understanding hypoxia-related pathophysiology provides avenues for therapeutic intervention in hypoxia-related disorders.

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Section: Methodsmentioning

confidence: 99%