Identification of Phospholipid Molecular Species in Porcine Brain Extracts Using High Mass Accuracy of 4.7 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Yu, Seonghyun; Cho, Kun; Kim, Young Hwan; Park, Soo‐Jin; Kim, Jae-Dong; Oh, Han Bin

doi:10.5012/bkcs.2006.27.5.793

Cited by 13 publications

(2 citation statements)

References 19 publications

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“…Seong-hyun et al, 2006) supplemented with 5 mol% brain PI(4,5)P 2 . If needed, 0.5 mole% BODIPY TR ceramide was present in the lipid mixture as a membrane reporter.…”

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confidence: 99%

Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53

Inamdar

Tsai

Dibsy

et al. 2021

eLife

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During HIV-1 particle formation, the requisite plasma membrane curvature is thought to be solely driven by the retroviral Gag protein. Here, we reveal that the cellular I-BAR protein IRSp53 is required for the progression of HIV-1 membrane curvature to complete particle assembly. SiRNA-mediated knockdown of IRSp53 gene expression induces a decrease in viral particle production and a viral bud arrest at half completion. Single molecule localization microscopy at the cell plasma membrane shows a preferential localization of IRSp53 around HIV-1 Gag assembly sites. In addition, we observe the presence of IRSp53 in purified HIV-1 particles. Finally, HIV-1 Gag protein preferentially localizes to curved membranes induced by IRSp53 I-BAR domain on giant unilamellar vesicles. Overall, our data reveal a strong interplay between IRSp53 I-BAR and Gag at membranes during virus assembly. This highlights IRSp53 as a crucial host factor in HIV-1 membrane curvature and its requirement for full HIV-1 particle assembly.

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“…Seong-hyun et al, 2006) supplemented with 5 mol% brain PI(4,5)P 2 . If needed, 0.5 mole% BODIPY TR ceramide was present in the lipid mixture as a membrane reporter.…”

mentioning

confidence: 99%

Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53

Inamdar

Tsai

Dibsy

et al. 2021

eLife

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“…In this kind of instrument, the ions are selected by their mass and categorized by differences in a magnetic field. Magnetic sector analyzers for phospholipid analysis include time of flight (TOF) (Schiller et al, 2004), ion trap , quadrupole (Han and Gross, 1994;Hsu and Turk, 2003), Fourier transform-ion ciclotron resonance (FT-ICR) (Yu et al, 2006), and hybrid instruments, for instance quadrupole-time of flight (QqTOF) (Ekroos et al, 2003), quadrupole ion trap (Garrett et al, 2011) and quadrupole ion trap-time of flight (QIT-TOF) (Hayasaka et al, 2008).…”

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confidence: 99%

Plackett-Burman Design and Fragmentation Studies to Assist the Comparison of Techniques used to Extract Phospholipids Prior to Regiospecific Characterization by Liquid Chromatography Mass Spectrometry

Araujo¹,

Zhu²,

Breivik³

et al. 2016

AJMC

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A Plackett-Burman design was used for screening 12 variables that could affect the mass spectrometry signals of phosphatidylcholines (PtdCho) and proposing optimal fragmentation conditions to study the fragmentation mechanisms of PtdCho standards. Under optimal conditions, three well-established methods for extracting phospholipids from biological samples, specifically liquid-liquid extraction (LLE), normal phase liquid chromatography (NPLC) and high performance thin layer chromatography (HPTLC), were compared in terms of the number of regiospecifically characterized PtdCho by means of liquid chromatography three-stage mass spectrometry (LCMS 3 ). The same number of PtdCho species (35) were characterized by LCMS 3 in the LLE and NPLC fractions, whereas only 15 in the HPTLC fraction.

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Current literature in mass spectrometry

2007

J. Mass Spectrom.

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Identification of Phospholipid Molecular Species in Porcine Brain Extracts Using High Mass Accuracy of 4.7 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Cited by 13 publications

References 19 publications

Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53

Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53

Plackett-Burman Design and Fragmentation Studies to Assist the Comparison of Techniques used to Extract Phospholipids Prior to Regiospecific Characterization by Liquid Chromatography Mass Spectrometry

Current literature in mass spectrometry

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