Identification of phospholipids in secretory granules of human submucosal gland respiratory cells.

Girod, Sabine; Fuchey, Claudette; Galabert, C.; Lebonvallet, S.; Bonnet, N.; Ploton, Dominique; Puchelle, Édith

doi:10.1177/39.2.1987263

Cited by 30 publications

(24 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…28,31,32 Some authors suggest the existence of conductive airway surfactant that has different biochemical and surface-tension properties compared to alveolar surfactant. 32,33 We have studied tissue samples that were snap frozen directly after collection and performed the Raman analysis without any preceding processing ͑e.g., dies, labels͒. Although some artifacts were recognized ͓see Figs.…”

Section: Discussionmentioning

confidence: 99%

Raman microspectroscopic mapping studies of human bronchial tissue

Koljenović¹,

Schut²,

Meerbeeck³

et al. 2004

J. Biomed. Opt.

View full text Add to dashboard Cite

Characterization of the biochemical composition of normal bronchial tissue is a prerequisite for understanding the biochemical changes that accompany histological changes during lung cancer development. In this study, 12 Raman microspectroscopic mapping experiments are performed on frozen sections of normal bronchial tissue. Pseudocolor Raman images are constructed using principal component analysis and K-means cluster analysis. Subsequent comparison of Raman images with histologic evaluation of stained sections enables the identification of the morphologic origin (e.g., bronchial mucus, epithelium, fibrocollagenous stroma, smooth muscle, glandular tissue, and cartilage) of the spectral features. Raman spectra collected from the basal side of epithelium consistently show higher DNA contributions and lower lipid contributions when compared with superficial epithelium spectra. Spectra of bronchial mucus reveal a strong signal contribution of lipids, predominantly triolein. These spectra are almost identical to the spectra obtained from submucosal glands, which suggests that the bronchial mucus is mainly composed of gland secretions. Different parts of fibrocollagenous tissue are distinguished by differences in spectral contributions from collagen and actin/myosin. Cartilage is identified by spectral contributions of glycosaminoglycans and collagen. As demonstrated here, in situ analysis of the molecular composition of histologic structures by Raman microspectroscopic mapping creates powerful opportunities for increasing our fundamental understanding of tissue organization and function. Moreover, it provides a firm basis for further in vitro and in vivo investigations of the biochemical changes that accompany pathologic transformation of tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Raman microspectroscopic mapping studies of human bronchial tissue

Koljenović¹,

Schut²,

Meerbeeck³

et al. 2004

J. Biomed. Opt.

View full text Add to dashboard Cite

show abstract

“…32 There is evidence for the alveolar origin of the phospholipids in tracheal aspirates, as well as for the local production of airway phospholipids by submucosal gland respiratory cells. 33,34 Consistent amounts of saline (2.0 mL) were used to clear tracheal aspirate secretions from the suction tubing in a standardized manner. Phospholipid concentrations and concentrations of potential protein inhibitors of surfactant in tracheal aspirate samples were not measured in this study.…”

Section: Discussionmentioning

confidence: 99%

Tracheal aspirate surface tension in babies with hyaline membrane disease: Effects of synthetic surfactant replacement

et al. 1998

View full text Add to dashboard Cite

“…From the alveolar spaces, pulmonary surfactant is carried up the airways due to the surface tension gradient and the mucociliary escalator 22, 24 . Local production and release of phospholipids by tracheal epithelial cells 25–26 and the submucosal glands of both human tracheal and bronchial biopsy specimens 27 have also been detected, thus both sources likely contribute to airway surfactant composition. While evidence pertaining to the origin of the CAS points to both alveolar and local sources 12, 28 , recent studies suggest that alveolar surfactant represents the largest proportion of CAS 22–23 .…”

Section: Methodsmentioning

confidence: 99%

Tensiometric and Phase Domain Behavior of Lung Surfactant on Mucus-like Viscoelastic Hydrogels

Schenck

Fiegel

2016

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Lung surfactant has been observed at all surfaces of the airway lining fluids and is an important contributor to normal lung function. In the conducting airways, the surfactant film lies atop a viscoelastic mucus gel. In this work, we report on the characterization of the tensiometric and phase domain behavior of lung surfactant at the air-liquid interface of mucus-like viscoelastic gels. Poly(acrylic acid) hydrogels were formulated to serve as a model mucus with bulk rheological properties that matched those of tracheobronchial mucus secretions. Infasurf® (Calfactant), a commercially available pulmonary surfactant derived from calf lung extract, was spread onto the hydrogel surface. The surface tension lowering ability and relaxation of Infasurf films on the hydrogels was quantified and compared to Infasurf behavior on an aqueous subphase. Infasurf phase domains during surface compression were characterized by fluorescence microscopy and phase shifting interferometry. We observed that increasing the bulk viscoelastic properties of the model mucus hydrogels reduced the ability of Infasurf films to lower surface tension and inhibited film relaxation. A shift in the formation of Infasurf condensed phase domains from smaller, more spherical domains to large, agglomerated, multilayer structures was observed with increasing viscoelastic properties of the subphase. These studies demonstrate that the surface behavior of lung surfactant on viscoelastic surfaces, such as those found in the conducting airways, differs significantly from aqueous, surfactant-laden systems.

show abstract

Identification of phospholipids in secretory granules of human submucosal gland respiratory cells.

Cited by 30 publications

References 6 publications

Raman microspectroscopic mapping studies of human bronchial tissue

Raman microspectroscopic mapping studies of human bronchial tissue

Tracheal aspirate surface tension in babies with hyaline membrane disease: Effects of synthetic surfactant replacement

Tensiometric and Phase Domain Behavior of Lung Surfactant on Mucus-like Viscoelastic Hydrogels

Contact Info

Product

Resources

About