Identification of Phosphorus Stress Related Proteins in the Seedlings of Dongxiang Wild Rice (Oryza Rufipogon Griff.) Using Label-Free Quantitative Proteomic Analysis

Abstract

Phosphorus (P) deficiency tolerance in rice is a complex character controlled by polygenes. Through proteomics analysis, we could find more low P tolerance related proteins in unique P-deficiency tolerance germplasm Dongxiang wild rice (Oryza Rufipogon, DXWR), which will provide the basis for the research of its regulation mechanism. In this study, a proteomic approach as well as joint analysis with transcriptome data were conducted to identify potential unique low P response genes in DXWR during seedlings. The results showed that 3589 significant differential accumulation proteins were identified between the low P and the normal P treated root samples of DXWR. The degree of change was more than 1.5 times, including 60 up-regulated and 15 downregulated proteins, 24 of which also detected expression changes of more than 1.5-fold in the transcriptome data. Through quantitative trait locus (QTLs) matching analysis, seven genes corresponding to the significantly different expression proteins identified in this study were found to be uncharacterized and distributed in the QTLs interval related to low P tolerance, two of which (LOC_Os12g09620 and LOC_Os03g40670) were detected at both transcriptome and proteome levels. Based on the comprehensive analysis, it was found that DXWR could increase the expression of purple acid phosphatases (PAPs), membrane location of P transporters (PTs), rhizosphere area, and alternative splicing, and it could decrease reactive oxygen species (ROS) activity to deal with low P stress. This study would provide some useful insights in cloning the P-deficiency tolerance genes from wild rice, as well as elucidating the molecular mechanism of low P resistance in DXWR.

Systemic adaptation of rice plants under low phosphate conditions and interaction with endophytic bacteria

“…Our results indicate that roots play a more critical role in Pi starvation than leaves. Our findings agree with Li et al (2010) and Deng et al (2022), where Dongxiang and Lagrue rice varieties upregulated the expression of Pi transporters OsPTs and acid phosphatase OsPAPs in roots. The upregulation of the OsPTs gene is responsible for Pi acquisition from the soil and mobilization of Pi within plants (Shin et al, 2004).…”

“…The six selected genes were involved in low-Pi-responsive pathways. The increase in their expression under low Pi conditions has also been reported in other studies (Zhou et al, 2008;Li et al, 2010;Deng et al, 2022). Significantly, the OsPAP21 gene expression increased approximately 140 times in plants grown in the P+113 medium compared to those grown in the P0 medium.…”

Molecular Regulation for Maintenance of Plant Phosphate Homeostasis in Crop Plants as Basis for Precision Genome Engineering

“…Two of these genes, LOC Os12g09620 and LOC Os03g40670, were found to be present at both the transcriptome and proteome levels. The thorough investigation revealed that in dealing with low P stress condition, DXWR employs various strategies which include enhancing the the expression of PAPs, modifying membrane location of P transporters, rhizosphere area, and AS, and decreasing ROS activity [68]. This research will provide some light on the molecular mechanisms underlying low P resistance in DXWR and enable cloning of the P-deficit tolerance genes from wild rice.…”