Identification of phytochemicals in Qingfei Paidu decoction for the treatment of coronavirus disease 2019 by targeting the virus-host interactome

Li, Yuyun; Wu, Yan; Li, Siyan; Li, Yibin; Zhang, Xin; Shou, Zeren; Gu, Shushi; Zhou, Chenliang; Xu, Daohua; Zhao, Kangni; Tan, Suiyi; Qiu, Jiayin; Pan, Xiaoyan; Li, Lin

doi:10.1016/j.biopha.2022.113946

Cited by 8 publications

(4 citation statements)

References 77 publications

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“…In our study, the proportion of Tregs and expression of TGF-β1 were increased in liver of mice treated with OA, which suggested the promotive effect of OA on the proportion of Tregs may be associated with increased TGF-β1. The inhibitory effect of OA on CCL2, CXCL1, or CXCL10 was demonstrated in other diseases [69][70][71], and we revealed it in Con A-induced AIH for the first time. The specific mechanism and target site of OA on Treg/Th17 were not clarified in this study.…”

Section: Discussionmentioning

confidence: 52%

Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation

et al. 2023

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Autoimmune hepatitis (AIH) is a kind of autoimmune disease mediated by T cells, and its incidence is gradually increasing in the world. Oroxylin A (OA) is one of the major bioactive flavonoids that has been reported to inhibit inflammatory. Here, an AIH model of mouse was induced by Concanavalin A (Con A). It found that serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were decreased in mice with the treatment of OA. Hematoxylin-eosin staining showed that the liver injury was attenuated by OA, and TUNEL staining indicated that the cells apoptosis of liver was weakened in mice with OA treatment. ELISA analysis of cytokines and chemokines suggested that OA reduced the expression of IL-6, IL-17A, CCL2, CXCL1 and CXCL10, but promoted the expression of IL-10 and TGF-β in mice. The mRNA levels of IL-17A in liver and spleen tissues were also significantly decreased, on the contrary, the mRNA levels of IL-10 in liver and spleen tissues were increased. The proportion of Treg/Th17 detected by flow cytometry revealed that OA promoted the differentiation of Treg and inhibited the differentiation of Th17 both in the liver and spleen. The results of this study demonstrated the inhibitory effects of OA on AIH-induced liver injury and the inflammatory response of AIH, and revealed that OA affected the balance of Treg/Th17 and shifted the balance toward Treg differentiation. It provided new potential drugs for the prevention of AIH.

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Section: Discussionmentioning

confidence: 52%

Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation

et al. 2023

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“…Supported by network pharmacology, Qingfei Paidu Decoction and its major herbs including Ephedra sinica, Bupleurum chinense, Pogostemon cablin, Cinnamomum cassia, and Scutellariae Radix displayed anti-oxidative, immuno-modulatory, and antiviral action by preventing a cytokine storm and regulating ACE2 receptor binding (9). Three active components (oroxylin A, hesperetin and scutellarin) of Qingfei Paidu Decoction significantly inhibited the release of IL-6, IL-1β, and CXCL-10 from THP-1 macrophages challenged with the SARS-CoV-2 S protein, which suggested that the anti-inflammatory effects of Qingfei Paidu Decoction might be attributed to the effects of these active components (10). Oroxylin A inhibits the entry of SARS-CoV-2 pseudovirus into target cells and prevents SARS-CoV-2 S protein-mediated cell-cell fusion by binding with the ACE2 receptor.…”

Section: Introductionmentioning

confidence: 94%

Herbal medicines exhibit a high affinity for ACE2 in treating COVID-19

Zhang

2023

BST

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as well as the release of new viral particles. Therefore, ACE2 should be a target to screen drugs inhibiting the replication and proliferation of SARS-COV-2.Recently, many herbal medicines have exhibited a high affinity for ACE2 in treating COVID-19 (3). These herbal medicines can target ACE2 to prevent SARS-CoV-2 from entering into host cells. The current work has summarized these herbal medicines including formulas, single herbs, and active ingredients, which have exhibited a high affinity for ACE2 in treating COVID-19 (Figure 1). This work may provide meaningful and useful information on further research to investigate the mechanisms of herbal medicines against SARS-CoV-2. Herbal formulasCOVID-19 is categorized as a pestilence according to traditional Chinese medicine (TCM) theory. Herbal medicines have been used to treat and prevent viral infections for thousands of years, and herbalists have

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“…Three components of QFPD, oroxylin A, hesperetin, and scutellarin, exhibited potent anti-viral activities against live SARS-CoV-2 and Human Coronavirus OC43 (HCoV-OC43) with IC 50 values ranging from 18.68 to 63.27 μM. Oroxylin A inhibited the entry of the SARS-CoV-2 pseudovirus into target cells and inhibited SARS-CoV-2 S-protein-mediated cell-cell fusion by binding to the ACE2 receptor [65] .…”

Section: Potential Mechanisms Through Which Tmtfs Affect Sars-cov-2 I...mentioning

confidence: 99%

“Three medicines and three formulas” in COVID-19: From bench to bedside

Lyu,

Xiao,

Wang

et al. 2023

Acupuncture and Herbal Medicine

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Chinese guideline has been proven effective in the fight against Coronavirus disease 2019 (COVID-19) during the epidemic spread globally. Traditional Chinese medicine (TCM) has been widely recognized for its effectiveness in alleviating symptoms, inhibiting disease deterioration, reducing mortality, and improving cure rate of COVID-19 patients. During the pandemic, “three medicines and three formulas” stood out from hundreds of registered clinical studies and became the highly recommended TCM for COVID-19 treatment. The “three medicines and three formulas” not only effectively relieve the clinical symptoms of fever, cough, fatigue, and phlegm, but also significantly shorten the time of nucleic acid negative conversion, improve lung computed tomography imaging feature and inflammation, ameliorate clinical biochemical indicators, and reduce sequelae. The potential pharmacological mechanisms of them are mainly relevant with the crosstalk of viral toxicity, endothelial damage, cytokine storm, immune response, and microthrombus. In brief, the clinical effects as well as the potential mechanisms of “three medicines and three formulas” on COVID-19 were systematically analyzed and summarized covering the whole stages of disease development, including virus invasion and replication, immune response and cytokine storm, and acute respiratory distress syndrome and multiple organ dysfunction syndrome. We hope that this review could provide theoretical basis and reference for in-depth understanding the positive role of “three medicines and three formulas” for COVID-19 treatment.

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Identification of phytochemicals in Qingfei Paidu decoction for the treatment of coronavirus disease 2019 by targeting the virus-host interactome

Cited by 8 publications

References 77 publications

Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation

Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation

Herbal medicines exhibit a high affinity for ACE2 in treating COVID-19

“Three medicines and three formulas” in COVID-19: From bench to bedside

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